Development and Validation of RP-HPLC Method for the Estimation of Alogliptin in API And Tablet Formulation

Abstract

The analytical method was developed and validated for determination of Alogliptin in bulk and pharmaceutical dosage forms by High performance liquid chromatography. The separation was carried out on Zorbax SB-Aq (250 x4.6mm,5µ ID) column. The mobile phase consists of 0.1% TFA Water : ACN in the ratio 62:38 at flow rate 1ml/min with diode array detector wavelength at 290nm.The column temperature was adjusted at 30º ± 0.5°C with injection volume 10µl.The retention time of Alogliptin was 3.06min. The linearity of the calibration curve was linear over the concentration range 25-75µg/ml (r2=1). The validation was carried out as per ICH guidelines. The development of method was easy, rapid, linear, precise, accurate and consistent.

Comparative Extraction and Quantifi cation of Myricetin from Leaves of Madhuca longifolia Using RP-HPLC

“…Leaf powder was filtered in sieve shaker for 20 minutes (CIP Machineries, Ahmedabad, GJ, India) to choose a uniform particle size. The powder was put through sieves with varied mesh sizes to achieve this (12,24,45,85, and 120 mesh, Swastika Electric and Scientific Works, Ambala, HR, India). The leaf powder that made it through 120 mesh sieves was gathered and put to use in additional extraction tests.…”

“…It has several uses in a variety of sectors, including the separation of active molecules and the evaluation of their quality and quantity. [5][6][7][8][9][10][11][12][13] Myricetin (3, 3', 4', 5, 5', 7-Hexahydroxyflavone) is a member of the flavonoid class of polyphenolic compounds. It is also mentioned in Madhuca longifolia (mahua), a member of the Sapotaceae family and well-known as Honey tree and Butter-nut tree.…”