Development and validation of spectrophotometric methods for determination of ceftazidime in pharmaceutical dosage forms

Abstract

Ceftazidime is (Z)-(7R)-7-[2-(2-aminothiazol-4-yl)-2-(1-carboxy-1-methoxyimino)acetamido]-3-(1-pyridiniomethyl)-3-cephem-4-carboxylate pentahydrate (CFZM) (1, 2). It is a third generation cephalosporin antibiotic characterized by a broad antibacterial spectrum and resistant to beta-lactamase producing organisms in addition to its antimicrobial activity against Streptococci, Staphylococci, Pneumococci, etc. Several analytical procedures are available in the literature for the analysis of ceftazidime, via high performance liquid chromatography (4-7), charge transfer complexation (8), and spectrophotometric methods (9-14). Most of the spectrophotometric methods reported earlier suffer from disadvantages like a narrow linearity range, requiring heating or extraction, long time for the reaction to complete, use of non-aqueous systems, low stability of the colored prod- Two spectrophotometric methods for the determination of ceftazidime (CFZM) in either pure form or in its pharmaceutical formulations are described. The first method is based on the reaction of 3-methylbenzothiazolin-2-one hydrazone (MBTH) with ceftazidime in the presence of ferric chloride in acidic medium. The resulting blue complex absorbs at l max 628 nm. The second method describes the reaction between the diazotized drug and N-(1--naphthyl)ethylenediamine dihydrochloride (NEDA) to yield a purple colored product with l max at 567 nm. The reaction conditions were optimized to obtain maximum color intensity. The absorbance was found to increase linearly with increasing the concentration of CFZM; the systems obeyed the Beer's law in the range 2-10 and 10-50 µg mL -1 for MBTH and NEDA methods, resp. LOD, LOQ and correlation coefficient values were 0.15, 0.79 and 0.50, 2.61. No interference was observed from common excipients present in pharmaceutical formulations. The proposed methods are simple, sensitive, accurate and suitable for quality control applications.

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“…Various UV spectrophotometric methods are reported for the analysis of Ceftazidime alone in presence of other drugs [38][39][40][41][42] .…”

Development and Validation of Uv Spectrophotometric Method for Determination of Ceftazidime in Bulk and Pharmaceutical Formulation

“…Literature survey revealed that few analytical methods are available for the individual estimation of ceftazidime in bulk drug and dosage formulations by HPLC in different pharmacopeia [3][4] . Few methods were reported for individual estimation of ceftazidime by voltammetry [5][6] , electrophoresis 7 , HPLC [8][9][10][11] and some method in visible region by UV-Vis Spectrophotometer [12][13][14][15][16][17][18] . But single estimation of this drug with mixture of methanol and distilled water as solvent has not been reported in bulk and in pharmaceutical formulation.…”

Development and Validation of High Performance Liquid Chromatographic Method for the Simultaneous Determination of Ceftazidime and Sulbactam in Spiked Plasma and Combined Dosage form-Zydotam

“…A literature survey revealed that several methods have been used for estimation of ceftazidime alone which includes Spectrophotometry [5][6][7][8][9] , High Performance Liquid Chromatography (HPLC) [10][11][12] and in combination with pyridine [13] Vancomycin [14] and cefepime [15] by HPLC. Sulbactam was assayed successfully by Spectrophotometry [16] , Capillary Isotachophoresis [17] , HPLC [18][19][20][21] and Gas Chromatography-Mass Spectrometry (GC-MS) [22] .…”