Development and Validation of UV Spectrophotometric Method for Determination of Eletriptan HBr

Syed, Shoaeb Mohammad; Marathe, Rajendra; Mahaparale, P. R.

doi:10.35652/igjps.2020.10109

Cited by 2 publications

(2 citation statements)

References 3 publications

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“…The mobile phase was prepared by using a 70:30 ratio of phosphate buffer and acetonitrile. 700 mL of buffer and 300 mL of acetonitrile were mixed thoroughly [38]. While the dilutions of stock solutions were made ranging from 10 to 50 μg mL À1 by using the mobile phase.…”

Section: Preparation Of Mobile Phasementioning

confidence: 99%

Method development and validation for simultaneous determination of Eletriptan hydrobromide and Itopride hydrochloride from fast dissolving buccal films by using RP-HPLC

Siddique

Sarfraz

Zaman

et al. 2023

AChrom

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One of the most effective, rapid, and simple methods reversed-phase high-performance liquid chromatography (RP-HPLC) was used for simultaneous development and validation of Eletriptan hydrobromide (ELE HBR) and Itopride hydrochloride (ITP HCL) in combination. The method was validated based on the regulations of United States Pharmacopeia (USP) and International Conference on Harmonization (ICH) guidelines. Separation of both drugs was achieved within approximately 5 min by using a mobile phase made up of a 70:30 ratio of phosphate buffer and acetonitrile having a flow rate of 1 mL min−1. Furthermore, a comprehensive study was conducted on precision, accuracy, linearity, inter-day, intra-day studies, an assay of formulated films, and stability studies of combined prepared film. Co-efficient of correlation ranged between 0.9993, and 0.9965 for ELE HBR and ITP HCL respectively. The accuracy of the developed method was accurate as drug recoveries in both cases of ITP HCL, and ELE HBR falls between (99.87, 99.96, and 99.84%) to (99.81, 99.12, and 98.44%) respectively having a concentration range of solutions between 10, 30 and 50 μg mL−1 dilution. Films developed by using both drugs in combination were then validated for assay studies, and it was found that substantial results of 99.05%, and 99.87% were found in the case of ITP HCL and ELE HBR respectively. The stability of the solution and mobile phase showed the method's accuracy as the results were 97% for ITP HCL and 99% for ELE HBR. The proposed method developed for simultaneous determination of ITP HCL and ELE HBR was developed and validation and no interaction of any excipient were found.

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Section: Preparation Of Mobile Phasementioning

confidence: 99%

Method development and validation for simultaneous determination of Eletriptan hydrobromide and Itopride hydrochloride from fast dissolving buccal films by using RP-HPLC

Siddique

Sarfraz

Zaman

et al. 2023

AChrom

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show abstract

“…The samples for the study were prepared in a before mentioned procedure for separate percent levels. The resulting solutions were subjected to analysis for recovery studies in percent with the aid of calibration curve [12][13][14][15][16][17] . From the calibration data obtained it was found that the regression coefficient was less than 1 which is within the limits of Beer lamberts' law.…”

Section: Recovery Studymentioning

confidence: 99%

Development and Validation of UV Spectrophotometric Method for the Estimation of Fluconazole in the Marketed Dosage Formulations

Syed¹,

Rp²

2020

PBJ

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The aim of current study was to develop and validate UV Spectrophotometric method for Fluconazole. Ultra Violet Spectroscopy was carried out at 260nm and samples were prepared with a solution of phosphate buffer pH 7.4. The linearity demonstrated a correlation coefficient of 0.998. The method was subjected to validity for the parameters as per ICH guidelines. Correctness, exactness, LOD, LOQ, recovery study and range were determined and the parameters were determined by performing the repeated experiments and proper sampling of the solution. The objective of selection of UV spectroscopy was the simplicity, time and economy of the method furthermore the sensitivity was also high by UV spectroscopy as HPLC technique is costly, time consuming and number of factors may affect the determination. It was observed that, the proposed method was linear, correct, repeatable, error free, selective, specific and cost effective proving the dependability of the method. More over same solvent was used throughout the experimental work and it was found that the method was free from any type of interference from any excipients and method was simple, rapid, precise, accurate and sensitive and can be applied in routine analysis of Fluconazole in single and combined form.

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Development and Validation of UV Spectrophotometric Method for Determination of Eletriptan HBr

Cited by 2 publications

References 3 publications

Method development and validation for simultaneous determination of Eletriptan hydrobromide and Itopride hydrochloride from fast dissolving buccal films by using RP-HPLC

Method development and validation for simultaneous determination of Eletriptan hydrobromide and Itopride hydrochloride from fast dissolving buccal films by using RP-HPLC

Development and Validation of UV Spectrophotometric Method for the Estimation of Fluconazole in the Marketed Dosage Formulations

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