Development of a chemiluminescent enzyme immunoassay for the determination of dexamethasone in milk

Abstract

An indirect competitive chemiluminescent enzyme-linked immunosorbent assay (CL-ELISA) for the determination of dexamethasone (DEX) was developed using soybean peroxidase (SbP) as an enzyme label. A mixture of 3-(10 0 -phenothiazinyl)-propane-1-sulfonate (SPTZ) and 4-morpholinopyridine (MORPH) was used as an enhancer of SbP-induced chemiluminescence. Varying the concentrations of the capture antigen (DEX-ovalbumin) and specific anti-DEX antibody, the conditions of the assay were optimized. The values of IC 10 , IC 50 and working range (IC 20 -IC 80 ) of the CL-ELISA of DEX were 0.02, 0.9, 0.08-9.3 ng mL À1 , respectively. It was shown that a pretreatment of cow milk samples by centrifugation and 25% methanol prevented the matrix effect of whole milk. The coefficient of variation (CV) and recovery values from the spiked milk samples estimated by the developed CL-ELISA were in the range of 2.2 to 9.9% and 82 to 142%, respectively.

Electrocatalytic oxidation and determination of dexamethasone at an Fe3O4/PANI–CuII microsphere modified carbon ionic liquid electrode

“…It is regularly employed as an anti-inammatory and immunosuppressive agent for the treatment of conditions such as inammation, allergy, and autoimmune conditions. 1,2 This medication has been added to the list of banned medications because of its misuse as a doping agent in sports such as cycling and horse racing to improve performance. 3 Therefore, development of new, sensitive, selective and simple analytical approaches and techniques for determining dexamethasone in biological uids such as plasma and urine is essential aer administration for efficient and safe use.…”

Development of a Simple Colorimetric Determination of Dexamethasone

“…DXP is a corticosteroid 2 utilized for many years as immunosuppression and anti-inf lammatory for the treatment of sensitivity, aggravation, intrinsic adrenal hyperplasia, and immune system conditions. 3 An inflammatory study at cultural circumstances exhibited that treatment with dexamethasone reduces the secretion of interleukin IL-8. 4 DXP and other derivatives of the glucocorticoid hydrocortisone family have been prohibited as per their abusing as doping drugs in sports to boost performance.…”

<p>Simultaneous Detection of VEGF and CEA by Time-Resolved Chemiluminescence Enzyme-Linked Aptamer Assay</p>

“…Chemiluminescent enzyme-linked immunoassay (CLEIA) has been widely used in analytical chemistry and food safety owing to its wide dynamic range, low background interference, high sensitivity, and specificity. [28][29][30] The traditional CLEIA is used to mark the catalyst or luminescent agent on the antigen or antibody. After a series of immune reactions, the content of the target is finally detected by measuring chemiluminescence (CL) signal.…”