Development of a Fertility Restorer for inap CMS (Isatis indigotica) Brassica napus Through Genetic Introgression of One Alien Addition

Abstract

Novel Brassica napus cytoplasmic male sterility (CMS) with carpelloid stamens (inap CMS) was produced by intertribal somatic hybridization with Isatis indigotica (Chinese woad), but its RF (restorer of fertility) gene(s) existed in one particular woad chromosome that was carried by one fertile monosomic alien addition line (MAAL) of rapeseed. Herein, the selfed progenies of this MAAL were extensively selected and analyzed to screen the rapeseed-type plants (2n = 38) with good male fertility and to produce their doubled haploid (DH) lines by microspore culture. From the investigation of fertility restoration in the F1 hybrids with inap CMS, one DH line (RF 39) was identified to adequately restore male fertility and likely carried one dominant RF gene. Specifically, this restorer produced brown pollen grains, similar to the woad and the MAAL, suggesting that this trait is closely linked with the RF gene(s) and serves as one phenotypic marker for the restorer. This restorer contained 38 chromosomes of rapeseed and no intact chromosomes of woad, but some DNA fragments of woad origin were detected at low frequency. This restorer was much improved for pollen and seed fertility and for low glucosinolate content. The successful breeding of the restorer for inap CMS rendered this new pollination control system feasible for rapeseed hybrid production.

A complete set of monosomic alien addition lines developed from <i>Gossypium anomalum</i> in a <i>Gossypium hirsutum</i> background: genotypic and phenotypic characterization

“…For example, a disomic substitution line from Orychophragmus violaceus in Brassica napus was selected by crossing the MAALs with a B. napus nullisomics expressing novel or specific traits ( Ding et al 2013 ). Additionally, a fertility restorer for cytoplasmic male sterility in Brassica napus was developed from doubled haploid lines of one fertile MAAL of rapeseed by microspore culture ( Li et al 2019 ). At present, we are working on creating chromosome segment substitution lines from G. anomalum using MAALs.…”

CMS systems in rapeseed and their use in the breeding of domestic hybrids

“…Феномен ЦМС, выражающийся в неспособности растения продуцировать жизнеспособную пыльцу и обусловленный экспрессией аберрантных (химерных) генов, возникающих в результате перестроек ми то хондриального генома, описан у различных видов растений (Hanson, Bentolila, 2004;Ivanov, Dymshits, 2007). ЦМС возникает в популяциях спонтанно (гомоплазматическая или аутоплазматическая ЦМС) либо при отдаленной (межвидовой, межродовой) половой или соматической гибридизации (аллоплазматическая ЦМС) (Li et al, 2019;Sang et al, 2019). Признак мужской стерильности супрессируют ядерные гены восстановления фертильности (Rf).…”

“…Большинство митохондриальных генов у носителей этого типа ЦМС получены от I. indigotica, и лишь один оказался рекомбинантным (Kang et al, 2017). Получена линия рапса, несущая ген Rf, интрогрессированный от донора митохондриальных генов (Li et al, 2019). Суммарные сведения о генетическом контроле наиболее изученных у рапса типов ЦМС приведены в таблице 2.…”

Unraveling the Genetic Basis of Fertility Restoration for Cytoplasmic Male Sterile Line WNJ01A Originated From Brassica juncea in Brassica napus

“…Another CMS system, inap CMS, was obtained via backcross of the somatic hybrid of Isatis indigotica (Chinese woad) and B. napus, with B. napus as the recurrent parent (Kang et al, 2017). A restorer line carrying a dominant Rf gene was successfully developed for inap CMS (Li et al, 2019). Further research is required to isolate their restorer genes and use them in rapeseed breeding.…”

“…napus as the recurrent parent ( Kang et al, 2017 ). A restorer line carrying a dominant Rf gene was successfully developed for inap CMS ( Li et al, 2019 ). Further research is required to isolate their restorer genes and use them in rapeseed breeding.…”