Development of a headspace gas chromatographic test for the quantification of 1- and 2-bromopropane in human urine

B’Hymer, Clayton; Cheever, Kenneth L.

doi:10.1016/j.jchromb.2004.10.045

Cited by 8 publications

(2 citation statements)

References 18 publications

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“…AcPrCys is an important metabolite of 1-BP excreted in urine (B’Hymer and Cheever 2005; Garner et al, 2006; Kawai et al, 2001). In this study, AcPrCys was not detected in the control groups in animal experiments or in epidemiological investigations, demonstrating the high specificity of this indicator.…”

Section: Discussionmentioning

confidence: 99%

Biomarkers of exposure and effect in the serum and urine of rats or workers exposed to 1-bromopropane

Zhang

Xiao

et al. 2022

Toxicol Ind Health

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Extensively used in several industries in China as a cleaning agent, 1-bromopropane (1-BP) has significant adverse effects on the central nervous system. However, neither its mechanism of action nor sensitive biomarkers related to it have been determined thus far. In this study, animal experiments and occupational surveys were performed to explore the typical exposure and effect biomarkers of neurotoxicity induced by 1-BP. Male Wistar rats were exposed to 0, 500, or 1000 ppm of 1-BP followed by pathological and biomarker analyses. An epidemiological survey was conducted on 71 workers each from 1-BP exposed and control groups. Serum and urine samples were collected for biomarker testing. cNSE represents neuron-specific enolase (NSE) in the cerebral cortex, where as sNSE represents NSE in the serum; similar terminology applies to S-100β, and cyclooxygenase-2 (COX-2). In rats exposed to 1000 ppm 1-BP, pathological changes were observed in Purkinje cells, lumbar gray matter, and tibiofibular nerve, while levels of cNSE, cS-100β, cCOX-2, sS-100β, and sCOX-2 were significantly elevated at different time checkpoints. In the 500 ppm group, cCOX-2, sNSE, and sCOX-2 levels were significantly elevated at different time checkpoints. 1-BP and N-acetyl-S-(n-propyl)-L-cysteine (AcPrCys) were detected in rat urine, and there was a correlation between the level of sNSE or sCOX-2 and AcPrCys in the 500 ppm group. In the occupational epidemiological study, a significant correlation between AcPrCys and exposure concentration was also detected. The findings of this study indicated that AcPrCys was a sensitive exposure biomarker of 1-BP in rats as well as occupational populations.

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Section: Discussionmentioning

confidence: 99%

Biomarkers of exposure and effect in the serum and urine of rats or workers exposed to 1-bromopropane

Zhang

Xiao

et al. 2022

Toxicol Ind Health

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“…The current study involves development of a procedure for an exposure biomarker for 1-BP. The general utility of analytical techniques for measuring urinary markers to evaluate 1-BP exposure has led to the development of tests which include the analysis of urinary bromine [27,28], 3-bromopropionic acid [29], 1-BP [30], or mercapturic acids as stable biomarkers [31][32][33][34]. The purpose of the work described here was to design a sensitive and specific method for the quantification of AcPrCys using HPLC ESI-MS Specific Ion Monitoring (SIM).…”

Section: Introductionmentioning

confidence: 99%

Development of an HPLC–MS procedure for the quantification of N-acetyl-S-(n-propyl)-l-cysteine, the major urinary metabolite of 1-bromopropane in human urine

Cheever

Marlow

B’Hymer

et al. 2009

Journal of Chromatography B

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1‐ und 2‐Brompropan – Bestimmung von 1‐ und 2‐Brompropan in Urin mittels dynamischer Headspace‐GC/MS [Biomonitoring Methods in German language, 2019]

Hartwig¹,

Arand²

2019

The MAK‐Collection for Occupational Health and Safety

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The working group “Analyses in Biological Materials” of the Permanent Senate Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area verified the presented biomonitoring method. The analytical method described herein permits the sensitive determination of both unmetabolised 1‐bromopropane and 2‐bromopropane in urine. Prior to the determination using GC‐MS in the Single‐Ion‐Mode (SIM), the analytes are extracted and enriched using Headspace Solid Phase Dynamic Extraction (SPDE). To this end, the urine samples are incubated at 50 °C and the analytes are extracted from the gas phase using SPDE. The headspace extract is then injected into the gas chomatograph for separation and mass spectrometric analysis. Calibration standards are prepared in urine and processed in the same way as the samples to be analysed. Deuterated benzene is used as the internal standard (IS).

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Development of a headspace gas chromatographic test for the quantification of 1- and 2-bromopropane in human urine

Cited by 8 publications

References 18 publications

Biomarkers of exposure and effect in the serum and urine of rats or workers exposed to 1-bromopropane

Biomarkers of exposure and effect in the serum and urine of rats or workers exposed to 1-bromopropane

Development of an HPLC–MS procedure for the quantification of N-acetyl-S-(n-propyl)-l-cysteine, the major urinary metabolite of 1-bromopropane in human urine

1‐ und 2‐Brompropan – Bestimmung von 1‐ und 2‐Brompropan in Urin mittels dynamischer Headspace‐GC/MS [Biomonitoring Methods in German language, 2019]

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