Development of a solid phase extraction procedure for HPLC–DAD determination of several angiotensin II receptor antagonists in human urine using mixture design

Ferreiròs, Nerea; Iriarte, Gorka; Alonso, Rosa M.; Jiménez, Roberto

doi:10.1016/j.talanta.2007.04.062

Cited by 36 publications

(21 citation statements)

References 28 publications

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“…Therefore, no addition of this second organic modifier was chosen and the percentage of TFA was fixed at 0.1% for each component of the mobile phase. Once the chromatographic separation had been optimised and the SPE procedure developed [27], validation of this new SPE -HPLC -DAD method for eprosartan, telmisartan, irbesartan, and valsartan in human urine samples was accomplished.…”

Section: Chromatographic Separation: Experimental Designmentioning

confidence: 99%

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Separation and quantitation of several angiotensin II receptor antagonist drugs in human urine by a SPE–HPLC–DAD method

Ferreiròs

Iriarte

Alonso³

et al. 2008

J of Separation Science

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In this work, an SPE-HPLC method coupled to photodiode array detection was validated in human urine matrix, in order to monitor four antihypertensive angiotensin II receptor antagonist drugs in patients under cardiovascular treatment. For that purpose, experimental design was used. Quantitation was accomplished by the internal standard method. The obtained LOQs were 95, 113, 125, and 85 ng/mL for eprosartan, telmisartan, irbesartan, and valsartan, respectively. The intraday and interday precision and accuracy at four concentration levels in the working range (LOQ-15 microg/mL) were always lower than 11% RSD and 8% relative error. The urine samples proved to be stable during 4 h at room temperature, after three thaw-freeze cycles, and for 2 months at -20 degrees C. No interferences from other endogenous compounds or co-administered drugs were found. The method has been successfully applied to monitor the renal elimination of eprosartan and valsartan during 24 h.

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Section: Chromatographic Separation: Experimental Designmentioning

confidence: 99%

“…The SPE procedure had been previously optimised in our laboratory by using mixture design [27]. Reagent grade TFA and sodium di-and monohydrogen phosphate were purchased from Merck (Darmstadt, Germany).…”

Section: Introductionmentioning

confidence: 99%

Separation and quantitation of several angiotensin II receptor antagonist drugs in human urine by a SPE–HPLC–DAD method

Ferreiròs

Iriarte

Alonso³

et al. 2008

J of Separation Science

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“…2 Medicines modifying reninangiotensin system activity include: drugs reducing renin release (β-adrenolytic drugs), drugs blocking conversion of angiotensin I to angiotensin II -ACE (angiotensin converting enzyme) inhibitors and angiotensin receptor antagonists (ARB -angiotensin II receptor blockers), also known as sartans. They are better in preventing first occurrence of atrial fibrillation than beta-blocker (atenolol) or calcium antagonist (amlodipine) therapy.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Sartans by High Performance Liquid Chromatography with Ultra Violet Detection

Maslarska¹,

Yankov²,

Obreshkova³

et al. 2017

IJPER

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Objective: A high performance liquid chromatographic method with ultra violet detection for simultaneous analysis of three sartans (Valsartan, Irbesartan and Telmisartan) has been developed for quality control. Method and Results: Isocratic elution on a LiChrospher C18 column (250 × 4 mm, particle size 5 µm) at the temperature 30ºC with a mobile phase consisting of 10mM phosphate buffer: acetonitrile (65:35 v/v) at a flow rate 1.0 ml/min has been done. The column eluent was monitored with a UV detector at 225 nm. This allowed a rapid detection and identification as well as quantitation of the eluting peaks. Method Validation: Calibration curves for all drugs were in the range of 5-40 µg/ml and the linear regression coefficients were more than 0.995. Recovery rates for the sartans were in the range 96.5% to 103.1%. The limits of detection were calculated between 0.04-0.06 µg/ ml. Also, the limits of quantification were 0.12-0.16 µg/ml. Within-day and between-day coefficient of variation for all sartans at all concentrations in the range of 0.83 -3.79% was calculated. Conclusion: The procedure can provide a simple, sensitive and fast method for the quality control of the three sartans in bulk and tablets.

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“…The terminal elimination half-life of irbesartan averaged 11-15 h. [1][2][3] Many analytical methods were reported in the literature for determination of irbesartan in pharmaceutical formulations or in biological fluids. These include high performance liquid chromatography (HPLC), [4][5][6][7][8][9][10] high-performance thinlayer chromatography (HPTLC), 11 liquid chromatographymass spectrometry (LC-MS), 12 spectrophotometry, [13][14][15][16][17][18] capillary electrophoresis 19 and voltammetry. 20,21 Almost all the reported methods have some drawbacks, lack in some validation parameters and necessitate time-consuming liquidliquid extraction steps prior to the analysis.…”

mentioning

confidence: 99%

“…20,21 Almost all the reported methods have some drawbacks, lack in some validation parameters and necessitate time-consuming liquidliquid extraction steps prior to the analysis. On the other hand, in most of the reported HPLC methods for routine clinical analysis, a high-throughput analysis with expensive solid-phase extraction [8][9][10][11] is always not advantageous as such equipment and techniques are not available in most of the laboratories. Moreover, the two reported voltammetric methods 20,21 did not offer a sufficient sensitive quantification limit of irbesartan especially in pharmacokinetic studies.…”

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confidence: 99%

Voltammetry of irbesartan drug in pharmaceutical formulations and human blood: quantification and pharmacokinetic studies

El‐Desoky¹,

Ghoneim²,

Habazy³

2011

J. Braz. Chem. Soc.

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Voltamogramas cíclicos de 'irbesatan' em um eletrodo de gota pendente de mercúrio no tampão Britton-Robinson com valores de pH menores do que 4,5 exibiram um único pico catódico irreversível envolvendo dois elétrons, correspondendo à redução-saturação da ligação dupla C=N de sua unidade tetrazólia. Na faixa de pH 4,5-5,5, os voltamogramas exibiram dois picos catódicos irreversíveis (1º e 2º picos) com corrente de pico menor porém com alturas relativas iguais, as quais foram atribuídas à redução do grupo C=N das formas ácida e básica de 'irbesatan', respectivamente. Um método voltamétrico de onda quadrada com redissolução anódica adsortiva foi desenvolvido para a quantificação de ibsertan total em solução. O método desenvolvido foi aplicado com sucesso na quantificação de 'ibsertan' em formulações farmacêuticas e soro humano enriquecido sem a necessidade de pré-tratamento de amostras e/ou etapas lentas de extração antes da análise. Foram obtidas interferências insignificantes de seu ingrediente ativo "hidroclorotiazida", excipientes, íons metálicos comuns e drogas co-administradas. Limites de detecção de 9,0x10-10 e 2,1x10 -9 mol L -1 e limites de quantificação de 3,0x10 -9 e 7,0x10 -9 mol L -1 de 'irbesartan' total em padrão e em soro humano enriquecido foram alcançadas, respectivamente, pelo método voltamétrico desenvolvido. Além disto, parâmetros fármacocinéticos de 'irbesartan' em plasma de voluntários saudáveis obtidos após a administração oral de uma única dose de tabletes Aprovel® também foram estimados.Cyclic voltammograms of irbesartan at the hanging mercury dropping electrode in the BrittonRobinson buffer of pH values lower than 4.5 exhibited a single 2-electron irreversible cathodic peak corresponding to the reduction-saturation of the C=N double bond of its tetrazolyl moiety. Over the pH range 4.5-5.5, the voltammograms exhibited two irreversible cathodic peaks (1 st and 2 nd peaks) of lower peak current but of relatively equal heights which were attributed to reduction of the C=N group of the acidic and basic forms of irbesartan, respectively. A validated square-wave adsorptive cathodic stripping voltammetric method was developed for quantification of irbesartan in the bulk form. The developed stripping voltammetric method was successfully applied for quantitation of irbesartan in pharmaceutical formulations and spiked human serum, without the necessity for samples pretreatment and/or time-consuming extraction steps prior to the analysis. Insignificant interferences from its active ingredient "hydrochlorothiazide", excipients, common metal ions and co-administrated drugs were obtained during the analysis. Limits of detection of 9.0x10 -10 and 2.1x10 -9 mol L -1 and limits of quantitation of 3.0x10 -9 and 7.0x10 -9 mol L -1 irbesartan in the bulk form and in spiked human serum were achieved, respectively, by the developed stripping voltammetric method. Moreover, pharmacokinetic parameters of irbesartan in plasma of healthy volunteers following an oral administration of a single dose of Aprovel® tablet...

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Development of a solid phase extraction procedure for HPLC–DAD determination of several angiotensin II receptor antagonists in human urine using mixture design

Cited by 36 publications

References 28 publications

Separation and quantitation of several angiotensin II receptor antagonist drugs in human urine by a SPE–HPLC–DAD method

Separation and quantitation of several angiotensin II receptor antagonist drugs in human urine by a SPE–HPLC–DAD method

Simultaneous Determination of Sartans by High Performance Liquid Chromatography with Ultra Violet Detection

Voltammetry of irbesartan drug in pharmaceutical formulations and human blood: quantification and pharmacokinetic studies

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