Separation and quantitation of several angiotensin II receptor antagonist drugs in human urine by a SPE–HPLC–DAD method

Ferreiròs, Nerea; Iriarte, Gorka; Alonso, Rosa M.; Jiménez, Roberto; Ortiz, E.

doi:10.1002/jssc.200700442

Cited by 29 publications

(18 citation statements)

References 29 publications

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“…In conclusion, by evaluating some key points of this on-line SPE-MS/MS method in comparison with previous methods developed for the quantitation of ARA-II in biological matrices and more particularly valsartan and candesartan [5,[7][8][9][11][12][13][14], we found that with the very low volume of biological sample used, it is among the most sensitive described methods. Moreover, to our knowledge it presents also the shortest analysis run time including chiefly the sample clean-up.…”

Section: System Ruggednessmentioning

confidence: 97%

“…Single analytes in biological matrices were extracted by liquid-liquid extraction [5], solid phase extraction (SPE) [6][7][8][9][10] or protein precipitation [11][12][13]. Extracts were assayed by liquid chromatography with ultra-violet absorbance detection (LC-UV) [6,7], fluorescence detection (LC-Fluo) [5,8,13] or mass spectrometry with electrospray ionization (ESI-MS) [9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

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Direct analysis of valsartan or candesartan in human plasma and urines by on-line solid phase extraction coupled to electrospray tandem mass spectrometry

Levi

Wuerzner

Ezan

et al. 2009

Journal of Chromatography B

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Section: System Ruggednessmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Direct analysis of valsartan or candesartan in human plasma and urines by on-line solid phase extraction coupled to electrospray tandem mass spectrometry

Levi

Wuerzner

Ezan

et al. 2009

Journal of Chromatography B

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“…Li et al [6] quantified HCTZ and another ARB, valsartan, by use of a tandem mass spectrometric method; their quantification limits were 1 and 4 ng mL -1 for HCTZ and valsartan, respectively. IRBE LOQ described in the literature are 0.78, 3, 15, and 125 ng mL -1 , respectively, for an LC-MS-MS method [8], fluorescence detection [15], solid-phase microextraction with HPLC-DAD [29], and an SPE-HPLC-DAD method for urine [30].…”

Section: Methods Validationmentioning

confidence: 99%

HPLC–DAD Analysis of Hydrochlorothiazide and Irbesartan in Hypertensive Patients on Fixed-Dose Combination Therapy

et al. 2011

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Hydrochlorothiazide (HCTZ) and the angiotensin II type 1 receptor antagonist (ARB) irbesartan (IRBE) are well-known antihypertensive drugs, frequently administered as a low-dose combination in a single pill. In this work, a simple, sensitive, and selective high-performance liquid chromatographic (HPLC) method with diodearray detection was developed for simultaneous determination of HCTZ and IRBE levels in the plasma of hypertensive patients given a fixed combination of 12.5 mg HCTZ and 300 mg IRBE. Compounds were extracted from acidified plasma samples with 3 mL ethyl acetate, and eluted at 6 and 19 min from a C 4 column by elution with an acetonitrile-phosphate buffer (pH 3.6) mobile-phase gradient at a flow rate of 1 mL min -1 . The assay was linear over the ranges 2.5-500 and 20-4,000 ng mL -1 for HCTZ and IRBE, respectively. Overall intra-assay and inter-assay variation were within acceptance limits. Limits of quantification were 2.5 and 20 ng mL -1 for HCTZ and IRBE, respectively. Plasma samples remained stable for 12 h at room temperature, through three thaw-freeze cycles, and for 2 and 7 months at -20°C. In hypertensive patients, residual concentrations were 22.3 ± 6.0 and 241.8 ± 39.0 ng mL -1 for HCTZ and IRBE, respectively. There was no interference from other co-administered drugs. Despite the different physicochemical properties of HCTZ and IRBE, our method enables accurate measurement of both drugs for assessment of compliance by patients treated by fixed-dose combination therapy with HCTZ-IRBE.

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“…Based on the recent literature, most of the publications deal with the determination of the following compounds in the ARB group -Valsartan, both for pharmaceutical formulations [3][4][5][6][7] and biological material: plasma 5,8 and urine. 9 It is normally determined by HPLC method with C18 column and UV-VIS detector 3,6 , mass spectrometry (MS) detector 5 or fluorescence detector. 4 It is also determined spectrophotometrically 9 and with TLC.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Sartans by High Performance Liquid Chromatography with Ultra Violet Detection

Maslarska¹,

Yankov²,

Obreshkova³

et al. 2017

IJPER

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Objective: A high performance liquid chromatographic method with ultra violet detection for simultaneous analysis of three sartans (Valsartan, Irbesartan and Telmisartan) has been developed for quality control. Method and Results: Isocratic elution on a LiChrospher C18 column (250 × 4 mm, particle size 5 µm) at the temperature 30ºC with a mobile phase consisting of 10mM phosphate buffer: acetonitrile (65:35 v/v) at a flow rate 1.0 ml/min has been done. The column eluent was monitored with a UV detector at 225 nm. This allowed a rapid detection and identification as well as quantitation of the eluting peaks. Method Validation: Calibration curves for all drugs were in the range of 5-40 µg/ml and the linear regression coefficients were more than 0.995. Recovery rates for the sartans were in the range 96.5% to 103.1%. The limits of detection were calculated between 0.04-0.06 µg/ ml. Also, the limits of quantification were 0.12-0.16 µg/ml. Within-day and between-day coefficient of variation for all sartans at all concentrations in the range of 0.83 -3.79% was calculated. Conclusion: The procedure can provide a simple, sensitive and fast method for the quality control of the three sartans in bulk and tablets.

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Separation and quantitation of several angiotensin II receptor antagonist drugs in human urine by a SPE–HPLC–DAD method

Cited by 29 publications

References 29 publications

Direct analysis of valsartan or candesartan in human plasma and urines by on-line solid phase extraction coupled to electrospray tandem mass spectrometry

Direct analysis of valsartan or candesartan in human plasma and urines by on-line solid phase extraction coupled to electrospray tandem mass spectrometry

HPLC–DAD Analysis of Hydrochlorothiazide and Irbesartan in Hypertensive Patients on Fixed-Dose Combination Therapy

Simultaneous Determination of Sartans by High Performance Liquid Chromatography with Ultra Violet Detection

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