Development of a system for S locus haplotyping based on the polymorphic SLL2 gene tightly linked to the locus determining self-incompatibility in radish (Raphanus sativus L.)

Kim, Daehyeon; Jung, Juyeon; Choi, Yeon-Ok; Kim, Sunggil

doi:10.1007/s10681-016-1681-7

Cited by 11 publications

(9 citation statements)

References 43 publications

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“…Additional S haplotypes have been identified in other Raphanus species (GenBank: DQ984139, EF056499, GQ121139, KP117076-KP110090). A new system was recently developed for haplotyping the S locus based on the polymorphic SLL2 gene tightly linked to the SI-determining locus in radish, resulting in the identification of 26 S haplotypes (Kim, Jung, Choi, & Kim, 2016). However, there is still a relative lack of S haplotypes in Chinese radish.…”

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confidence: 99%

Identification and classification of S haplotypes in radish (Raphanus sativus)

Wang

Zheng²,

Zhang

2018

Plant Breeding

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Radish (Raphanus sativus L.) is a typical cross‐pollinated crop that exhibits obvious heterosis. Self‐incompatibility is an important character for F1 hybrid breeding of radish. Knowledge of the S haplotypes of breeding lines is very important for breeders to avoid cross‐incompatibility of the parental lines. In the present study, the S haplotypes of 63 radish inbred lines, which were independently cultivated by our research group, were identified by PCR amplification, sequencing and BLAST analyses of the SRK and SLG genes. Finally, fifty‐four inbred lines were classified into 15 class I S haplotypes, including three new types, RsS‐38, RsS‐39 and RsS‐40. Additionally, three class II S haplotypes were identified in nine radish inbred lines. Partial SRK or SLG sequences were completed, such as RsS‐11 Lim (SRK‐S), RsS‐26 (SRK‐K), RsS‐5 Lim (SRK‐K and SRK‐S) and RsS‐9 (SRK‐K). The identified S haplotypes were verified with a cross‐pollination test, and RsS‐9 has weaker self‐incompatibility than other S haplotypes. These information will not only contribute to the production of hybrid seeds but also to the development of new self‐compatible inbred lines, which were advantageous of the production of maintain line and male line in CMS breeding system.

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confidence: 99%

Identification and classification of S haplotypes in radish (Raphanus sativus)

Wang

Zheng²,

Zhang

2018

Plant Breeding

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“…PCR amplification of an SRK-like sequence was also reported by Kim and Kim (2018) and Kim and Kim (2019). Kim and Kim (2019) reported that RsSRK-like8, which was identified from a radish draft genome as an SRK homolog by Kim et al (2016), was amplified by the class-II primer set as a non-S locus gene. Moreover, it was reported that RsKD1, which was identified from another radish draft genome as a gene showing high sequence identity in the kinase domain of the SRK, might complicate S genotyping because RsKD1 is a non-S locus gene and could be amplified with class-II SRK primers (Kim and Kim, 2018).…”

Section: Resultsmentioning

confidence: 70%

“…However, only two different S alleles, RsS 2 and RsS 10 , were detected in Eisai (Supplementary Table S3). This may have been due to the findings in previous studies that some SRK alleles in radish are difficult to detect because of nucleotide sequence diversity in the S locus genes (Okamoto et al, 2004;Kim et al, 2016;Haseyama et al, 2018).…”

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confidence: 99%

Spatiogenetic characterization of S receptor kinase (SRK) alleles in naturalized populations of Raphanus sativus L. var. raphanistroides on Yakushima island

et al. 2021

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“…S haplotypes can be classified into two groups, class I and class II, based on amino acid sequence similarities of SLG and SRK [ 16 ]. The amino acid sequences of the S domain of SRK or SLG alleles show a 72% similarity within the same group and a similarity of 70% or less between different groups [ 17 ]. Additionally, SLG and the S domain of SRK alleles within the same S haplotype generally show high sequence similarity, especially in the class II group [ 18 , 19 , 20 , 21 ].…”

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confidence: 99%

Development of S Haplotype-Specific Markers to Identify Genotypes of Self-Incompatibility in Radish (Raphanus sativus L.)

Heo,

Kim,

et al. 2024

Plants

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Radish (Raphanus sativus L.), a root vegetable belonging to the Brassicaceae family, is considered one of the representative crops displaying sporophytic self-incompatibility (SSI). The utilization of a self-incompatibility system in F1 breeding can improve the efficiency of cross-combinations, leading to a reduction in breeding time and aiding in the development of novel F1 varieties. The successful implementation of this system necessitates the rapid and accurate identification of S haplotypes in parental lines. In this study, we identified a total of nine S haplotypes among 22 elite radish lines through Sanger sequencing. Subsequently, we obtained sequences for showing a 95% similarity to nine S haplotypes, along with sequences identified by other researchers using BLAST. Following this, multiple sequence alignment (MSA) was conducted to identify SRK and SLG sequence similarities, as well as polymorphisms within the class I and II groups. Subsequently, S haplotype-specific marker sets were developed, targeting polymorphic regions of SRK and SLG alleles. These markers successfully amplified each of the nine S haplotypes. These markers will play a crucial role in the rapid and precise identification of parental S haplotypes in the radish F1 breeding process, proving instrumental in the radish F1 purity test.

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Development of a system for S locus haplotyping based on the polymorphic SLL2 gene tightly linked to the locus determining self-incompatibility in radish (Raphanus sativus L.)

Cited by 11 publications

References 43 publications

Identification and classification of S haplotypes in radish (Raphanus sativus)

Identification and classification of S haplotypes in radish (Raphanus sativus)

Spatiogenetic characterization of <i>S</i> receptor kinase (<i>SRK</i>) alleles in naturalized populations of <i>Raphanus sativus</i> L. var. <i>raphanistroides</i> on Yakushima island

Development of S Haplotype-Specific Markers to Identify Genotypes of Self-Incompatibility in Radish (Raphanus sativus L.)

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