Development of a universal conductive platform for anchoring photo- and electroactive proteins using organometallic terpyridine molecular wires

Jacquet, Margot; Izzo, Miriam; Osella, Silvio; Kozdra, Sylwia; Michałowski, Paweł Piotr; Gołowicz, Dariusz; Kazimierczuk, Krzysztof; Gorzkowski, Maciej T.; Lewera, Adam; Teodorczyk, M.; Trzaskowski, Bartosz; Jurczakowski, Rafał; Gryko, Daniel T.; Kargul, Joanna

doi:10.1039/d0nr08870f

Cited by 10 publications

(5 citation statements)

References 89 publications

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“…This has been done to compare the performance of ITO/NiO(OH) electrode with the bare ITO substrate using EIS technique. The calculated value of R s and R s + R ct were found to be 278.3 Ω and 889.7 Ω, respectively, which yielded a very high value of R ct as 611.4 Ω, for bare ITO and this has good agreement with previous reports. , The results clearly indicate that the bare ITO substrate exhibits more solution and charge transfer resistance toward DA under this test condition, making it more inert regarding charge transfer reactions to occur on its surface between the ITO electrode and DA. The resistive nature of blank ITO substrate was also reflected in low current density observed from CV profiles of it in the presence of only PBS and DA with PBS (Figure a).…”

Section: Resultssupporting

confidence: 88%

Electrochemically Grown Hole-Rich NiO(OH) Thin Films toward Hole-Mediated Very Fast and Selective Enzyme-Free Electrochemical Sensing of Dopamine under Simulated Environment

Mondal,

Khan,

Ahmed

et al. 2024

ACS Appl. Bio Mater.

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This work aimed to develop an enzyme-free semiconductor-assisted electrochemical technique for the selective detection of the neurotransmitter dopamine. In this case, electrochemically grown nickel oxyhydroxide [NiO(OH)] thin films were chosen to fabricate the sensing platform, i.e., the electrodes. Chronoamperometry was used to deposit the films on indium tin oxide (ITO) coated glass substrates. The films were thoroughly characterized to establish their structure, composition, phase purity, and electrochemical attributes. Electrochemical sensing characteristics were investigated by means of cyclic and differential pulse voltammetry, steady-state amperometry, and electrochemical impedance spectroscopy. The effects of several interfering agents like glucose, sodium chloride, methanol, hydrogen peroxide, and paracetamol were also studied on the detection attributes of dopamine. Significantly high value of sensitivity (11.87 μA μM −1 cm −2 ) was obtained for dopamine sensing that was associated with a limit of detection (LoD) of 0.22 μM of dopamine. However, the sensitivity (2.51 μA μM −1 cm −2 ) and LoD (1.20 μM) obtained for serotonin were inferior compared to those of dopamine. The performance of the electrode toward dopamine sensing was not compromised either in the presence of only serotonin or a series of other electroactive interfering agents, which makes the electrode very much dopamine selective. The dopamine response time was 200 ms, which is notably fast. Extensive studies on the effect of temperature, pH and scan rate on the detection of dopamine by the developed electrode material have also been carried out. The developed electrodes were also found to be notably stable for dopamine detection with a decay of only 6.6% in oxidation peak current density after the 50th cycle. Real-life application of the developed electrode material was checked with urine samples from adult male humans and yielded encouraging results.

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Section: Resultssupporting

confidence: 88%

Electrochemically Grown Hole-Rich NiO(OH) Thin Films toward Hole-Mediated Very Fast and Selective Enzyme-Free Electrochemical Sensing of Dopamine under Simulated Environment

Mondal,

Khan,

Ahmed

et al. 2024

ACS Appl. Bio Mater.

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“…Second, the probability of electric short-circuiting of electrons transferred from PSI to electrolyte should be limited. The latter limitation can be overcome through nano-structuring of the PSI complexes within thin films, e.g., through the use of DNA origami [56], the orienting biotic conductive thin layers (made of, e.g., cytochrome c; [14,[57][58][59]) or organic molecular wires [60,61].…”

Section: Discussionmentioning

confidence: 99%

Competition between intra-protein charge recombination and electron transfer outside photosystem I complexes used for photovoltaic applications

Goyal

Szewczyk

Burdziński

et al. 2022

Photochem Photobiol Sci

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Photosystem I (PSI) complexes isolated from three different species were electrodeposited on FTO conducting glass, forming a photoactive multilayer of the photo-electrode, for investigation of intricate electron transfer (ET) properties in such green hybrid nanosystems. The internal quantum efficiency of photo-electrochemical cells (PEC) containing the PSI-based photo-electrodes did not exceed ~ 0.5%. To reveal the reason for such a low efficiency of photocurrent generation, the temporal evolution of the transient concentration of the photo-oxidized primary electron donor, P+, was studied in aqueous suspensions of the PSI complexes by time-resolved absorption spectroscopy. The results of these measurements provided the information on: (1) completeness of charge separation in PSI reaction centers (RCs), (2) dynamics of internal charge recombination, and (3) efficiency of electron transfer from PSI to the electrolyte, which is the reaction competing with the internal charge recombination in the PSI RC. The efficiency of the full charge separation in the PSI complexes used for functionalization of the electrodes was ~ 90%, indicating that incomplete charge separation was not the main reason for the small yield of photocurrents. For the PSI particles isolated from a green alga Chlamydomonas reinhardtii, the probability of ET outside PSI was ~ 30–40%, whereas for their counterparts isolated from a cyanobacterium Synechocystis sp. PCC 6803 and a red alga Cyanidioschyzon merolae, it represented a mere ~ 4%. We conclude from the transient absorption data for the PSI biocatalysts in solution that the observed small photocurrent efficiency of ~ 0.5% for all the PECs analyzed in this study is likely due to: (1) limited efficiency of ET outside PSI, particularly in the case of PECs based on PSI from Synechocystis and C. merolae, and (2) the electrolyte-mediated electric short-circuiting in PSI particles forming the photoactive layer, particularly in the case of the C. reinhardtii PEC. Graphical abstract

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“…Finally, to obtain more atomic-level information, we performed molecular dynamics (MD) studies, (for other computational studies of electrochemical experiments on proteins see [65] , [66] , [67] , [68] ). We prepared models of ACE2 and CD147 receptors on the functionalised sensor (see Methods) and performed 500 ns MD runs.…”

Section: Resultsmentioning

confidence: 99%

Enhanced susceptibility of SARS-CoV-2 spike RBD protein assay targeted by cellular receptors ACE2 and CD147: Multivariate data analysis of multisine impedimetric response

Brodowski¹,

Pierpaoli²,

Janik³

et al. 2022

Sensors and Actuators B: Chemical

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Development of a universal conductive platform for anchoring photo- and electroactive proteins using organometallic terpyridine molecular wires

Abstract: The construction of an efficient conductive interface between electrodes and electroactive proteins is a major challenge in the biosensor and bioelectrochemisty fields to achieve the desired nanodevice performance. Concomitantly, metallo-organic...

Cited by 10 publications

References 89 publications

Electrochemically Grown Hole-Rich NiO(OH) Thin Films toward Hole-Mediated Very Fast and Selective Enzyme-Free Electrochemical Sensing of Dopamine under Simulated Environment

Electrochemically Grown Hole-Rich NiO(OH) Thin Films toward Hole-Mediated Very Fast and Selective Enzyme-Free Electrochemical Sensing of Dopamine under Simulated Environment

Competition between intra-protein charge recombination and electron transfer outside photosystem I complexes used for photovoltaic applications

Enhanced susceptibility of SARS-CoV-2 spike RBD protein assay targeted by cellular receptors ACE2 and CD147: Multivariate data analysis of multisine impedimetric response

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