Development of amylose-free (amf) monoploid potatoes as new basic material for mutation breeding in vitro

Hoogkamp, T.J.H.; Ende, R. G. T. Van Den; Jacobsen, E.; Visser, Richard G. F.

doi:10.1007/bf02357958

Cited by 6 publications

(3 citation statements)

References 19 publications

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“…For sequence analyses DNA was used from five monoploid potato genotypes: 7322 (H7322 or AM79.7322, originally from G. Wenzel, Institüt für Genetik, Grünbach, Germany, see: De Vries et al 1987 ; Hovenkamp-Hermelink et al 1988 ), M5 and M38 (851-5 and 851-38, Uijtewaal 1987 ), M47 and M133 (1022 M-47 and 1022 M-133, Hoogkamp et al 2000 ). DNA from 20 monoploid S. phureja and S. chacoense clones was obtained from Richard Veilleux (Blacksburg, Virginia, USA, see Lightbourn and Veilleux 2007 ).…”

Section: Methodsmentioning

confidence: 99%

Identification of alleles of carotenoid pathway genes important for zeaxanthin accumulation in potato tubers

Wolters¹,

Uitdewilligen²,

Kloosterman³

et al. 2010

Plant Mol Biol

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We have investigated the genetics and molecular biology of orange flesh colour in potato (Solanum tuberosum L.). To this end the natural diversity in three genes of the carotenoid pathway was assessed by SNP analyses. Association analysis was performed between SNP haplotypes and flesh colour phenotypes in diploid and tetraploid potato genotypes. We observed that among eleven beta-carotene hydroxylase 2 (Chy2) alleles only one dominant allele has a major effect, changing white into yellow flesh colour. In contrast, none of the lycopene epsilon cyclase (Lcye) alleles seemed to have a large effect on flesh colour. Analysis of zeaxanthin epoxidase (Zep) alleles showed that all (diploid) genotypes with orange tuber flesh were homozygous for one specific Zep allele. This Zep allele showed a reduced level of expression. The complete genomic sequence of the recessive Zep allele, including the promoter, was determined, and compared with the sequence of other Zep alleles. The most striking difference was the presence of a non-LTR retrotransposon sequence in intron 1 of the recessive Zep allele, which was absent in all other Zep alleles investigated. We hypothesise that the presence of this large sequence in intron 1 caused the lower expression level, resulting in reduced Zep activity and accumulation of zeaxanthin. Only genotypes combining presence of the dominant Chy2 allele with homozygosity for the recessive Zep allele produced orange-fleshed tubers that accumulated large amounts of zeaxanthin.Electronic supplementary materialThe online version of this article (doi:10.1007/s11103-010-9647-y) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Identification of alleles of carotenoid pathway genes important for zeaxanthin accumulation in potato tubers

Wolters¹,

Uitdewilligen²,

Kloosterman³

et al. 2010

Plant Mol Biol

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“…To aid haplotype identification five monoploid potato reference genotypes were used: 7322 (H7322, or AM79.7322 originally from G. Wenzel, Institut für Genetik, Grünbach, Germany), M47 and M133 (1022M-47 and 1022M-133) (Hoogkamp et al 2000 ) and M5 and M38 (851-5 and 851-38) (Uijtewaal 1987 ). Furthermore, DNA from nine diploid reference genotypes was used: C and E (US-W5337.3 and 77.2102.37) (Hanneman Jr and Peloquin 1967 ; Jacobsen 1980 ), 1024-2 and 1029-31 (87.1024/2 and 87.1029/31) (Jacobsen et al 1989 ), RH and SH (RH89-039-16 and SH82-93-488) (Rouppe Van Der Voort et al 1997 ; Van Os et al 2006 ), RH90 and RH88 (RH90-038-21 and RH88-025-50) (Park et al 2005 ), and G254 (Olsder and Hermsen 1976 ).…”

Section: Methodsmentioning

confidence: 99%

Allelic variation for alpha-Glucan Water Dikinase is associated with starch phosphate content in tetraploid potato

et al. 2022

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Key message Association analysis resulted in the identification of specific StGWD alleles causing either an increase or decrease in starch phosphate content which was verified in diploid and tetraploid potato mapping populations. Abstract Potatoes are grown for various purposes like French fries, table potatoes, crisps and for their starch. One of the most important aspects of potato starch is that it contains a high amount of phosphate ester groups which are considered to be important for providing improved functionalization after derivatization processes. Little is known about the variation in phosphate content as such in different potato varieties and thus we studied the genetic diversity for this trait. From other studies it was clear that the phosphate content is controlled by a quantitative trait locus (QTL) underlying the candidate gene α-Glucan Water Dikinase (StGWD) on chromosome 5. We performed direct amplicon sequencing of this gene by Sanger sequencing. Sequences of two StGWD amplicons from a global collection of 398 commercial cultivars and progenitor lines were used to identify 16 different haplotypes. By assigning tag SNPs to these haplotypes, each of the four alleles present in a cultivar could be deduced and linked to a phosphate content. A high value for intra-individual heterozygosity was observed (Ho = 0.765). The average number of different haplotypes per individual (Ai) was 3.1. Pedigree analysis confirmed that the haplotypes are identical-by-descent (IBD) and offered insight in the breeding history of elite potato germplasm. Haplotypes originating from introgression of wild potato accessions carrying resistance genes could be traced. Furthermore, association analysis resulted in the identification of specific StGWD alleles causing either an increase or decrease in starch phosphate content varying from 12 nmol PO4/mg starch to 38 nmol PO4/mg starch. These allele effects were verified in diploid and tetraploid mapping populations and offer possibilities to breed and select for this trait.

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“…Starches without amylose make brown-red complex, as iodine binds only weakly to the short helical segments in amylopectin molecules. Spectrophotometric assays were developed based on this complexation 4 5 but most importantly, this technique provides an important HTP screening opportunity and permitted the identification of low amylose potato lines by screening of thousands of lines in a mutant collection 6 . This method is also valuable for identifying starches that resists amylolytic degradation, so called resistant starch or RS.…”

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confidence: 99%

High throughput screening of starch structures using carbohydrate microarrays

Tanackovic

Rydahl

Pedersen

et al. 2016

Sci Rep

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In this study we introduce the starch-recognising carbohydrate binding module family 20 (CBM20) from Aspergillus niger for screening biological variations in starch molecular structure using high throughput carbohydrate microarray technology. Defined linear, branched and phosphorylated maltooligosaccharides, pure starch samples including a variety of different structures with variations in the amylopectin branching pattern, amylose content and phosphate content, enzymatically modified starches and glycogen were included. Using this technique, different important structures, including amylose content and branching degrees could be differentiated in a high throughput fashion. The screening method was validated using transgenic barley grain analysed during development and subjected to germination. Typically, extreme branching or linearity were detected less than normal starch structures. The method offers the potential for rapidly analysing resistant and slowly digested dietary starches.

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Development of amylose-free (amf) monoploid potatoes as new basic material for mutation breeding in vitro

Cited by 6 publications

References 19 publications

Identification of alleles of carotenoid pathway genes important for zeaxanthin accumulation in potato tubers

Identification of alleles of carotenoid pathway genes important for zeaxanthin accumulation in potato tubers

Allelic variation for alpha-Glucan Water Dikinase is associated with starch phosphate content in tetraploid potato

High throughput screening of starch structures using carbohydrate microarrays

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