Development of an HPLC–MS/MS method for the selective determination of paracetamol metabolites in mouse urine

Hewavitharana, Amitha K.; Lee, S.; Dawson, Paul A.; Markovich, Daniel; Shaw, P. Nicholas

doi:10.1016/j.ab.2007.11.011

Cited by 46 publications

(28 citation statements)

References 15 publications

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“…The total clearance (CL) was found to be 1.2 ± 0.2 L h -1 kg -1 with an elimination half-life of 2.6 ± 0.4 h. The clearance found in this study is 4 times more than that reported by Parthasarathy et al [20]. The lower clearance reported by the previous authors may be due to the lack of specificity associated with the UV detection which resulted in higher plasma concentrations and an under estimation of apparent clearance [17,30].…”

Section: Application To Pharmacokinetic Studycontrasting

confidence: 73%

“…This may be explained by saturation of plasma protein binding (dose in the present study is 3 times higher than the previous study), in addition to distribution into highly perfused tissues as a result of the lipophilic nature of mitragynine [28,29]. The other reason could be an over estimation of drug plasma concentration due to the lack of UV detection specificity compared to MS [30]. The total clearance (CL) was found to be 1.2 ± 0.2 L h -1 kg -1 with an elimination half-life of 2.6 ± 0.4 h. The clearance found in this study is 4 times more than that reported by Parthasarathy et al [20].…”

Section: Application To Pharmacokinetic Studycontrasting

confidence: 48%

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Simple, Sensitive, High-Throughput Method for the Quantification of Mitragynine in Rat Plasma Using UPLC-MS and Its Application to an Intravenous Pharmacokinetic Study

et al. 2011

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A simple, sensitive and rapid ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) method was developed and validated for the quantification of mitragynine in rat plasma using amitriptyline hydrochloride as an internal standard. Sample preparation involved a one-step liquid-liquid extraction using methyl t-butyl ether. Mitragynine was separated on an Acquity UPLC TM BEH HILIC column using isocratic elution with a mobile phase of 10 mM ammonium formate buffer containing 0.1% formic acid:acetonitrile (15:85, v/v). At a flow rate of 0.2 mL min -1 , the retention time of mitragynine was found to be 1.3 min. Ionization was performed in the positive ion electrospray mode. The selected mass-tocharge (m/z) ratio transition of mitragynine ion [M ? H] ? used in the selected ion recording (SIR) was 399.1. The calibration curve was found to be linear over a concentration range of 1-5,000 ng mL -1 (r = 0.999) with a lower limit of quantification (LLOQ) of 1 ng mL -1 . Intra-and inter-day assay variations were found to be less than 15%. The extraction recoveries ranged from 85-93% at the three concentrations (2, 400 and 4,000 ng mL -1 ) in rat plasma.This method was successfully used to quantify mitragynine in rat plasma following intravenous administration of the compound.

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Section: Application To Pharmacokinetic Studycontrasting

confidence: 73%

Section: Application To Pharmacokinetic Studycontrasting

confidence: 48%

Simple, Sensitive, High-Throughput Method for the Quantification of Mitragynine in Rat Plasma Using UPLC-MS and Its Application to an Intravenous Pharmacokinetic Study

et al. 2011

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“…Specially interesting is the metabolism of PAR in young infants and neonates, when the volume of biological sample taken to analyze is limited. Therefore the simple HPLC microanalytical techniques were then applied [7]. The other method reported is capillary electrophoresis with ultraviolet and mass spectrometric detection that was used for the determination of PAR with PG and PS in urine and serum samples [8].…”

Section: Introductionmentioning

confidence: 99%

The Preliminary Studies of Electrochemical Behavior of Paracetamol and Its Metabolites on Glassy Carbon Electrode by Voltammetric Methods

Baranowska

Koper

2009

Electroanalysis

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The new, rapid and sensitive method for the determination of paracetamol (PAR) and its glucuronide (PG) and sulfate (PS) metabolites is proposed. The electrochemical properties of the compounds were examined by cyclic voltammetry (CV) on glassy carbon electrode (GCE). All measurements were carried out in Britton -Robinson buffers (BR) with different pH values over the pH range 1.81 -7.24. The preliminary research indicated that PAR could be determined simultaneously with one of its metabolites. The linearity of calibration curves was obtained for concentrations between 1.65 Â 10 À5 and 1.65 Â 10 À4 M for PAR, 1.53 Â 10 À5 and 1.53 Â 10 À4 M for PG and 2.17 Â 10 À5 and 1.52 Â 10 À4 M for PS.

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“…Several analytical techniques have been employed for the determination of PAR, such as thin layer chromatography [6], high performance liquid chromatography [7], liquid chromatography tandem mass spectroscopy [8,9], capillary electrophoresis [10], electrochemical analysis [4,11], chemiluminescence [12], spectrophotometry [13], Raman spectrometry and Fourier transform infrared spectrometry [14]. However, these methods have several drawbacks such as environmentally unfriendly solvents, requirement for sample pretreatment, expensive instrumentation and in some cases long analysis time that makes them unsuitable for routine analysis [4,15].…”

Section: Introductionmentioning

confidence: 99%

Determination of Paracetamol Based on its Quenching Effect on the Photoluminescence of CdTe Fluorescence Probes

Ding

Cheng

et al. 2011

J Fluoresc

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L-Cysteine capped CdTe nanoparticles (NPs) were synthesized in aqueous medium, and their application as fluorescence probes in the determination of paracetamol was studied. The L-cysteine capped CdTe NPs were characterized by transmission electron microscopy, X-ray diffraction spectrometry, spectrofluorometry, ultraviolet-visible and Fourier transform infrared spectrometry. Based on the distinct fluorescence quenching of CdTe fluorescence probes in the presence of paracetamol, a simple, rapid and specific method for paracetamol determination was presented. Under optimum conditions, the relative fluorescence intensity of CdTe NPs was linearly proportional to paracetamol concentration from 1.0 × 10(-8) mol/L to 1.6 × 10(-7) mol/L with a detection limit of 4.2 × 10(-9) mol/L. The proposed method was applied to detect paracetamol in commercial tablets with satisfactory results.

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Development of an HPLC–MS/MS method for the selective determination of paracetamol metabolites in mouse urine

Cited by 46 publications

References 15 publications

Simple, Sensitive, High-Throughput Method for the Quantification of Mitragynine in Rat Plasma Using UPLC-MS and Its Application to an Intravenous Pharmacokinetic Study

Simple, Sensitive, High-Throughput Method for the Quantification of Mitragynine in Rat Plasma Using UPLC-MS and Its Application to an Intravenous Pharmacokinetic Study

The Preliminary Studies of Electrochemical Behavior of Paracetamol and Its Metabolites on Glassy Carbon Electrode by Voltammetric Methods

Determination of Paracetamol Based on its Quenching Effect on the Photoluminescence of CdTe Fluorescence Probes

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