Development of an Immunoassay for Monitoring the Levels of Ciprofloxacin Patient Samples

Abstract

It has been traditional to measure drug concentrations using high pressure liquid chromatography (HPLC). While this method is highly accurate, it is time consuming and requires the use of appropriate standards for identification of the compound. In addition, identification and quantification of drugs from patient samples requires significant manipulation to remove protein. In contrast, enzyme-linked immunoassays (EIA) are able to assay samples with a high degree of specificity, and are able to process multiple samples at a time. In addition, serum proteins do not interfere with sample quantification and samples may be tested without significant preparation. We describe the development of an EIA for the detection of ciprofloxacin in serum and dialysate samples. The immunoassay is specific for ciprofloxacin and is sensitive for picogram amounts of the antibiotic.

A single spectroscopic flow-through sensing device for determination of ciprofloxacin

“…Numerous methods have been reported for the determination of ciprofloxacin using techniques such as spectrophotometry (indirectly [1,2]; by means of a charge transfer complex formation [3]; by using its Cu(II) complex and derivative spectrophotometry [4], direct UV spectrophotometry [5] or derivative UV spectrophotometry [6]); high performance liquid chromatography with UV detection [7]; capillary electrophoresis [8][9][10] and immunoassay [11]. Automatic methods based on flow injection analysis (FIA) have also been proposed using various spectroscopic detection techniques: spectrophotometry [12], luminescence (spectrofluorimetry [13,14], phosphorimetry [15] and chemiluminescence [16]).…”

Determination of fluoroquinolone antibiotic levofloxacin in urine by fluorescence polarization immunoassay

“…They are used in the treatment of various infectious diseases; often it is important to maintain the therapeutic concentration of an active substance in blood to perform optimum treatment. There are two versions of such an approach: the determination of the active substance in serum [1] or plasma, or the control of its elimination with urine. Serum is a very complicated sample for analysis because of its complex composition and, as a conse quence, strong matrix effect.…”

Preconcentration and Determination of Ciprofloxacin with Solid-phase Microextraction and Silica-coated Magnetic Nanoparticles Modified with Salicylic Acid by UV-Vis Spectrophotometry

“…The HPTLC method has been developed for the determination and the purity control of CF in coated tablets [14,15]. Other different methods were reported such as spectrophotometry [16][17][18][19][20][21][22], fluorimetry [22][23][24], capillary electrophoresis [25][26][27][28][29][30][31][32] and immunoassay [33,34]. Both spectrophotometric [35][36][37][38] and chemiluminescent [39,40] detection were proposed as automatic spectroscopy procedures.…”