Development of electrochemical DNA biosensors

Liu, Ailin; Wang, Kun; Weng, Shaohuang; Lei, Yun; Lin, Lin; Chen, Wei; Chen, Yuanzhong

doi:10.1016/j.trac.2012.03.008

Cited by 165 publications

(85 citation statements)

References 94 publications

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“…Table 3 lists the biosensors based on CB reported up to date. Electrochemical biosensors can be constructed exploring different biological events in according to the target analyte and biological recognition element, ranging from biocatalytic assays (enzymatic biosensors) [73] to affinity assays (genosensors and immunosensors) [74,75]. In analyzing Table 3, it is evident that CB has been mainly applied for enzymatic biosensors.…”

Section: Biosensors Based On Nanostructured Carbon Blackmentioning

confidence: 99%

Electrochemical Biosensors Based on Nanostructured Carbon Black: A Review

Silva

Moraes

Janegitz

et al. 2017

Journal of Nanomaterials

133

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Carbon black (CB) is a nanostructured material widely used in several industrial processes. This nanomaterial features a set of remarkable properties including high surface area, high thermal and electrical conductivity, and very low cost. Several studies have explored the applicability of CB in electrochemical fields. Recent data showed that modified electrodes based on CB present fast charge transfer and high electroactive surface area, comparable to carbon nanotubes and graphene. These characteristics make CB a promising candidate for the design of electrochemical sensors and biosensors. In this review, we highlight recent advances in the use of CB as a template for biosensing. As will be seen, we discuss the main biosensing strategies adopted for enzymatic catalysis for several target analytes, such as glucose, hydrogen peroxide, and environmental contaminants. Recent applications of CB on DNA-based biosensors are also described. Finally, future challenges and trends of CB use in bioanalytical chemistry are discussed.

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Section: Biosensors Based On Nanostructured Carbon Blackmentioning

confidence: 99%

Electrochemical Biosensors Based on Nanostructured Carbon Black: A Review

Silva

Moraes

Janegitz

et al. 2017

Journal of Nanomaterials

133

View full text Add to dashboard Cite

show abstract

“…12 Electrochemical methods gained much attention among other methods due to their high sensitivity, low cost instrumentation, fast response, simplicity and portability in detection. 13 The sensitivity of electrochemical DNA sensors highly depends on the signal amplification; thus, suitable sensing materials must be carefully applied. 14 Unique properties of nanomaterials have contributed to unlimited options of sensing materials.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Polylactide-Stabilized Gold Nanoparticles and Its Application in the Fabrication of Electrochemical DNA Biosensors

Nordin¹,

Yusof²,

Abdullah³

et al. 2016

Journal of the Brazilian Chemical Society

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In this work, two different approaches to gold nanoparticles (AuNPs) have been explored for the modification of screen-printed electrode based on AuNPs and AuNPs stabilized with polylactic acid (PLA). The modified substrate has been characterized using UV-Vis spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), field emission scanning electron microscopy (FESEM) and cyclic voltammetry. Both synthesized AuNPs were studied in terms of stability, sensitivity and reproducibility to enhance the sensing capability of modified electrodes. The PLA-stabilized AuNPs form strong structured nanoparticles and stabilize in aqueous solution. A larger active surface area (0.41 cm 2 ) and lower charge transfer resistance (R ct ) value were reported in the modification of sensing material with PLA-stabilized AuNPs, which resulted in enhancement of sensitivity. Therefore, AuNPs in PLA can be used as a potential alternative modifier for sensing chemicals and biomolecules in electrochemical sensors.

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“…Hvastkovs et al summarized the recent advances in electrochemical DNA hybridization detection [27]. Liu et al reviewed the development of electrochemical DNA biosensors and the future prospects [28]. Electrochemical DNA sensors have been used in food safety monitoring and the detection of pathogen DNA.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical DNA sensor for Staphylococcus aureus nuc gene sequence with zirconia and graphene modified electrode

Sun

Wang

et al. 2015

J Solid State Electrochem

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In this paper, zirconia (ZrO 2 ) and graphene (GR) nanocomposite was electrodeposited on the surface of carbon ionic liquid electrode (CILE), which was used to construct an electrochemical DNA biosensor. GR was electroreduced from graphene oxide by potentiostatic method, and ZrO 2 nanoparticle was further electrodeposited on GR/CILE by cycling voltammetric scan in a ZrOCl 2 solution. The presence of GR on the electrode surface can provide a highly conductive interface with large surface area for the loading of ZrO 2 nanoparticles. Single-stranded DNA (ssDNA) probe sequences with phosphate group at the 5′ end could be easily immobilized on the surface of ZrO 2 /GR/CILE due to the strong affinity between ZrO 2 and phosphate groups. The ssDNA/ZrO 2 /GR/CILE was applied to hybridize with the target ssDNA sequence, and methylene blue (MB) was used as the electrochemical indicator. Due to the different binding models of MB with double-stranded DNA and ssDNA on the electrode surface, electrochemical response of MB was decreased after the hybridization reaction. Under the optimal conditions, the reduction peak current of MB was proportional to the concentration of Staphylococcus aureus nuc gene sequence in the range from 1.0×10 −13 to 1.0×10 −6 mol L −1 with the detection limit of 3.23 × 10 −14 mol L −1 (3σ). The electrochemical DNA sensor exhibited good selectivity to various mismatched ssDNA sequences, and the polymerase chain reaction amplification products of S. aureus nuc gene sequence were further detected with satisfactory results. Therefore, this electrochemical DNA sensor with ZrO 2 nanoparticles and GR nanosheet modified electrode could be used for the detection of specific ssDNA sequence in real biological samples.

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Development of electrochemical DNA biosensors

Cited by 165 publications

References 94 publications

Electrochemical Biosensors Based on Nanostructured Carbon Black: A Review

Electrochemical Biosensors Based on Nanostructured Carbon Black: A Review

Characterization of Polylactide-Stabilized Gold Nanoparticles and Its Application in the Fabrication of Electrochemical DNA Biosensors

Electrochemical DNA sensor for Staphylococcus aureus nuc gene sequence with zirconia and graphene modified electrode

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