DEVELOPMENT OF ELEMENTS OF TECHNOLOGY FOR PLANTING MATERIAL OF LETTUCE (&lt;i&gt;LACTUCA SATIVA L.&lt;/i&gt;) ON VIRUS-FREE BASIS USING METHODS OF BIOTECHNOLOGY

Романова, О. В.; Солдатенко, А. В.; Чичварина, О. А.; Ахраменко, В. А.; Павлова, О. В.; Романов, В. С.

doi:10.18619/2072-9146-2019-2-22-26

Cited by 3 publications

(1 citation statement)

References 22 publications

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“…В настоящее время разработаны технологии получения удвоенных гаплоидов через культуру микроспор in vitro для семейства капустные [22], включая капусту белокочанную [23,24], брокколи [25], репу [26], капусту пурпурную [27], горчицу сарептскую, индау посевной [28], редис европейский [29,30]. Разработан базовый протокол культуры микроспор in vitro для сортов салата (Lactuca sativa L.) [31]. Технология получения удвоенных гаплоидов моркови столовой разработана, как в культуре пыльников, в культуре неопыленных семяпочек [32] так и в культуре микроспор in vitro [33].…”

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The patriarch of Russian vegetable growing has a centenary

et al. 2020

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The article is dedicated to the 100th anniversary of the head institution of the Federal Scientific Vegetable Center - the oldest and leading breeding organization for vegetable crops, better known in the scientific and socio-economic spheres of the Russian Federation by its historical name - VNIISSOK. The center's activity dates back to the creation in 1920 of the Gribovskaya Vegetable Breeding Experimental Station, where, under the leadership of Professor Sergei Ivanovich Zhegalov, a theoretical and practical basis for the development of domestic breeding was laid. In 1970, the experimental station was transformed into the AllUnion Research Institute of Selection and Seed Production of Vegetable Crops, and in 1992 into the All-Russian Research Institute of Selection and Seed Production of Vegetable Crops. Over the years, breeding methods have been developed and improved, including modern biotechnological methods, using molecular marking and interspecies hybridization. The results obtained during this period reflect the traditions of scientific schools and approaches laid down by scientists-breeders, seed growers, vegetable growers, flower growers, both in the field of basic research, and in the creation of varieties and hybrids of vegetable crops. The organizational and structural reform of breeding institutions carried out in 2017 contributed to the formation of the Federal scientific vegetable center (FSBSI FSVC), which includes VNIISSOK as the parent organization and 8 branches located in various ecological and geographical zones. At a new stage of development, the FSBSI FSVC continues its glorious traditions. Scientists have obtained world-class results in basic research and competitive selection achievements in priority applied research. The scientific results and structural changes that have occurred over the hundred-year period of the breeding establishment are the foundation for the further development of the vegetable science in the country.

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The patriarch of Russian vegetable growing has a centenary

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REGENERATION SYSTEM OF LETTUCE (<i>LACTUCA SATIVA</i> L.) THROUGH <i>IN VITRO</i> CULTURE

2019

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The article is devoted to the problem of obtaining regenerative plant varieties of lettuce (Lactuca sativa L.) Bouquet, Emerald, Chameleon (FSBSI Federal Scientific Vegetable Center). Salad culture is insensitive to in vitro conditions and requires the development of alternative regeneration conditions. Seeds were surface sterilized in 96% ethanol (30 s), then 15 minutes in 50% aqueous solution of "Whiteness" with the addition of Twin-20 (1 drop per 100 ml), washed three times in sterile distilled water. Then lettuce seeds were placed in Petri dishes on Gamborg B5 medium (2% sucrose, 3,0 g/l fitogeli). After 10 days, the obtained seedlings were cut, the leaf blades were cut into squares 1 cm in size and transferred to medium MS B5 (2% sucrose) with three variants of hormone concentrations: 0.1 mg/l NAA and 1 mg/l BAP, 1 mg/l NAA and 1 mg/l BAP, 1 mg/l NAA and 0.1 mg/l BAP. The formed shoots were rooted on MS medium (2% sucrose, 7,0 g/l agar). Cultivation was carried out on racks with fluorescent lamps at 25°C and a photoperiod of 14 hours, illumination of 2,5 thousand Lux. According to the results of studies, the concentration of hormones (0,1 mg/l NAA, 1 mg/l BAP) was selected, which allowed to obtain up to 10 shoots from one leaf explant. Moreover, the largest number of shoots was noted on leaf explants located closer to the veins of the sheet. The obtained data allowed to improve the system of regeneration of varieties of emerald lettuce, Bouquet and Chameleon.

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Food independence and technological sovereignty of Russia in the vegetable growing sector

Pivovarov,

Soldatenko,

Pyshnaya

et al. 2024

Ovoŝi Rossii

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Relevance. The state of Russia's agro-industrial complex determines the country's national security, as it provides the population with quality agricultural products and raw materials, which affects state sovereignty. According to a number of analysts, in commercial vegetable growing, about 80 % of sown areas are annually sown with imported varieties and hybrids, and this is a vulnerability in ensuring the industry's independence and the country's competitiveness. Strengthening technological sovereignty in the field of vegetable production is one of the priorities of the state agrarian policy, as well as the main focus of scientific organisations and private companies.Results. Federal State Budgetary Scientific Institution Federal Scientific Vegetable Center (FSBSI FSVC) is a major scientific center of the industry, which can compete with foreign companies with its breeding achievements. The article shows the leading position in the breeding of vegetable and melon crops FSBSI FSVC, which actively conducts research on private genetics, biotechnology, contributing to the acceleration of the breeding process; varieties and hybrids are created that combine consistently high productivity, early maturity, resistance to biotic and abiotic stressors, high quality products with an optimal content of biologically active substances to obtain potential raw materials and products of functional action; development of resource-saving, environmentally safe, high-precision technologies for cultivation of vegetable and melon crops, taking into account species and varietal characteristics, as well as scientific bases and practical recommendations for production of original seeds taking into account zonal specifics.However, there are problems hindering the introduction of breeding achievements: the increased cost of registration of breeding achievements in the State Register of Selection Achievements of the Russian Federation of the Russian Federation, bureaucratic difficulties in their approbation, which arose for breeders after the adoption of the new law "On Seed Production". In order to solve the problems of seed production, the Ministries in charge of vegetable production have launched a sub-programme "Development of breeding and seed production of vegetable crops", which aims to increase the production of domestic competitive seeds and brings together the state, science and business.

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DEVELOPMENT OF ELEMENTS OF TECHNOLOGY FOR PLANTING MATERIAL OF LETTUCE (<i>LACTUCA SATIVA L.</i>) ON VIRUS-FREE BASIS USING METHODS OF BIOTECHNOLOGY

Cited by 3 publications

References 22 publications

The patriarch of Russian vegetable growing has a centenary

The patriarch of Russian vegetable growing has a centenary

REGENERATION SYSTEM OF LETTUCE (<i>LACTUCA SATIVA</i> L.) THROUGH <i>IN VITRO</i> CULTURE

Food independence and technological sovereignty of Russia in the vegetable growing sector

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