Development of glycolipid biosurfactant for inducing apoptosis in HeLa cells

Ramalingam, Vaikundamoorthy; Varunkumar, Krishnamoorthy; Ravikumar, Vasulinga T.; Rajendran, R.

doi:10.1039/c6ra00805d

Cited by 17 publications

(7 citation statements)

References 35 publications

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“…Finally, the plates were observed under bright field inverted light microscopy (Nikon, Japan) at 40× magnification. The morphological changes of lung cancer cells were documented 53 .…”

Section: Methodsmentioning

confidence: 99%

Target delivery of doxorubicin tethered with PVP stabilized gold nanoparticles for effective treatment of lung cancer

Ramalingam

Varunkumar

Ravikumar

et al. 2018

Sci Rep

142

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Development of drug delivery system conjugated with doxorubicin (dox) on the surface of AuNPs with polyvinylpyrrolidone (Dox@PVP-AuNPs), we have demonstrated that human lung cancer cells can significantly overcome by the combination of highly effective cellular entry and responsive intracellular release of doxorubicin from Dox@PVP-AuNPs complex. Previously drug release from doxorubicin-conjugated AuNPs was confirmed by the recovered fluorescence of doxorubicin from quenching due to the nanosurface energy transfer between doxorubicinyl groups and AuNPs. Dox@PVP-AuNPs achieved enhanced inhibition of lung cancer cells growth than free Doxorubicin and PVP-AuNPs. The in vitro cytotoxic effect of PVP-AuNPs, free Dox and Dox@PVP-AuNPs inhibited the proliferation of human lung cancer cells with IC50 concentration. Compared with control cells, PVP-AuNPs and free Dox, Dox@PVP-AuNPs can increases ROS generation, sensitize mitochondrial membrane potential and induces both early and late apoptosis in lung cancer cells. Moreover, Dox@PVP-AuNPs highly upregulates the expression of tumor suppressor genes than free Dox and PVP-AuNPs and induces intrinsic apoptosis in lung cancer cells. From the results, Dox@PVP-AuNPs can be considered as an potential drug delivery system for effective treatment of human lung cancer.

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“…Finally, the plates were observed under bright field inverted light microscopy (Nikon, Japan) at 40× magnification. The morphological changes of lung cancer cells were documented 53 .…”

Section: Methodsmentioning

confidence: 99%

Target delivery of doxorubicin tethered with PVP stabilized gold nanoparticles for effective treatment of lung cancer

Ramalingam

Varunkumar

Ravikumar

et al. 2018

Sci Rep

142

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“…The cytotoxic activity of methanol extracts of R. apiculata was evaluated using MTT assay (Ramalingam et al 2016). Lung cancer cells (1 × 10 −4 cells/ml) were taken into 96-well culture plate and incubated for 48 h at 37 °C in a humidified atmosphere of 95% air and 5% CO 2 .…”

Section: Mtt Assaymentioning

confidence: 99%

Enhanced antimicrobial, antioxidant and anticancer activity of Rhizophora apiculata: An experimental report

Ramalingam

Rajendran

2018

3 Biotech

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The present study is designed to evaluate the antimicrobial, antioxidant and anticancer activities of . Initially, the phenolic and flavonoid content was quantified in solvent extracts, and gallic acid and rutin were used as a control, respectively. Further, antimicrobial and minimal inhibitory activities of different solvent extracts were assessed against human clinical pathogenic bacteria, and the results showed that butanol and methanol extract has potential antimicrobial activity. FTIR analysis of solvent extracts showed the presence of phenolic compounds at 3409-3430 cm that actively involved in various applications including antioxidant and anticancer activities. The in vitro antioxidant activity of solvent extracts showed excellent antioxidant potential, about 84% of DPPH free-radical scavenging, 76% of hydrogen peroxide, 82% of hydroxyl radical scavenging, and 80% of reducing power. Two-way ANOVA analysis showed that the highly significant effect of antioxidant activity depends on the concentration of extracts. The DNA protection efficiency of extracts against oxidative damage was confirmed by DNA nicking assay using bacterial DNA. The methanol extract effectively inhibited the growth and induces the apoptosis through ROS generation and sensitizes the mitochondrial membrane potential of A549 lung cancer cells. Taken together, the results showed that the solvent extracts of could be potential antioxidant and anticancer agents.

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“…Thus, every treatment condition was repeated in triplicate and IC 50 of EPI-GNPs encapsulated liposomes obtained were used for treatment for all further studies.2.10. Analysis of apoptotic cells by Acridine Orange/ Ethidium bromide (AO/EB) double stainingApoptosis of the cancer cells were investigated by fluorescence microscopy after staining with AO/EB[25]. Briefly, MCF-7 cancer cells were seeded on a cover slip in a 24-well plate (1×10 5 cells/well),2.11.…”

mentioning

confidence: 99%

Preparation of liposomes encapsulated Epirubicin-gold nanoparticles for Tumor specific delivery and release

Kunjiappan

Panneerselvam²,

Somasundaram

et al. 2018

Biomed. Phys. Eng. Express

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Liposome/metal nanoparticle conjugates are found to be a promising carrier tool for effectively delivering wide variety of drugs in a targeted site and without arguing toxic side effects of existing anticancer drugs. In this scenario, the present work was performed to choose Epirubicin-HCl (EPI) as model anticancer drug to formulate citrate stabilized EPI-gold nanoparticles (EPI-GNPs). Further, the biocompatible EPI-GNPs incorporated with lipid bilayer of liposomes formulated to exhibit effective drug delivery system. The biosynthesized EPI-GNPs and its incorporation in a lipid bilayer of liposomes was confirmed by UV-visible spectrophotometer, drug encapsulation, loading capacity, pH stability and drug releasing capacity were studied. Simultaneously, drug and carrier interaction, physical nature, stability, morphological and particle size characteristics were also studied by FTIR, XRD, SEM, TEM and Zeta size analyzer. The obtained characterization data confirmed the properties of EPI-GNPs encapsulated liposomes. The in vitro cytotoxic screening of EPI-GNPs encapsulated liposomes arrest the proliferation of MCF-7 breast cancer cell lines, at 0.1 μg/ml of liposomes displayed 52% of cell death at 24 h. The EPI-GNPs encapsulated liposomes induced time and dose dependent apoptosis compared with untreated control cancer cells.

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Development of glycolipid biosurfactant for inducing apoptosis in HeLa cells

Cited by 17 publications

References 35 publications

Target delivery of doxorubicin tethered with PVP stabilized gold nanoparticles for effective treatment of lung cancer

Target delivery of doxorubicin tethered with PVP stabilized gold nanoparticles for effective treatment of lung cancer

Enhanced antimicrobial, antioxidant and anticancer activity of Rhizophora apiculata: An experimental report

Preparation of liposomes encapsulated Epirubicin-gold nanoparticles for Tumor specific delivery and release

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