Development of High-Performance Liquid Chromatography Method for the Simultaneous Analysis of Amlodipine and Valsartan in Combined Dosage Form and in Vitro Dissolution Studied

Yuryeva, Olga; Kondratova, Yuliya; Logoyda, Liliya

doi:10.22159/ajpcr.2018.v11i5.24443

Cited by 12 publications

(12 citation statements)

References 6 publications

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“…One ml of sample was withdrawn and replaced with fresh dissolution medium at the time intervals of 5, 15, 30, 45 min. The concentrations of enalapril in samples were determined by the proposed HPLC method [28].…”

Section: In Vitro Dissolution Studiesmentioning

confidence: 99%

Efficient Validated Method of HPLC to Determine Enalapril in Combinated Dosage Form Containing Enalapril and Bisoprolol and in Vitro Dissolution Studies

Logoyda¹

2019

Int J App Pharm

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Objective: A rapid and reproducible HPLC method has been developed for the determination of enalapril in experimental combined dosage forms containing bisoprolol and enalapril and for drug dissolution studies. Methods: The separation was done using a column Hi Qsil C18 (4.6х250 mm, 5 μm particle size) and a mobile phase of methanol: phosphate buffer solution (65:35, v/v), flow-rate of 1.0 ml/min. The injection volume was 300 μL and the ultraviolet detector was set at 225 nm. Results: The method was validated as per ICH guidelines. Under these conditions, enalapril was eluted at 5.33 min. Total run time was shorter than 6 min. A linear relationship between the concentration and the area of chromatographic peaks of enalapril in the range of 1.250 mg/ml–10.000 mg/ml (7.500 mg/ml at pH 1.2) was established. Requirements for linear dependency parameters are performed in this case throughout the range of application of the technique. Linearity studies were conducted in a wide range of concentrations (25-200% at pH 4.5 and 6.8, 25-150% at pH 1.2). In the medium with pH 1.2 release of enalapril from tablets in 5 min is 83.38 %, after 15 min-94.11%, after 30 min-97.17 %; in medium with pH 4.5 the release of enalapril from tablets in 5 min makes 47.13 %, after 15 min-88.34 %, after 30 min-95.86 %; in a medium with pH 6.8, the release of enalapril from tablets in 5 min is 71.04 %, and after 15 min-88.88 %, after 30 min-92.11 %. Conclusion: A simple and sensitive HPLC method was developed for the estimation of enalapril in tablets containing enalapril and bisoprolol. The proposed method was applied successfully for quality control assay of enalapril in experimental tablets and in vitro dissolution studies.

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Section: In Vitro Dissolution Studiesmentioning

confidence: 99%

Efficient Validated Method of HPLC to Determine Enalapril in Combinated Dosage Form Containing Enalapril and Bisoprolol and in Vitro Dissolution Studies

Logoyda¹

2019

Int J App Pharm

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“…For a successful study of this substance by these methods, usually, derivatization of active groups with one or more reagents is carried out. [3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21] However, as practice shows, in case of using tianeptine for the purpose of obtaining a narcotic effect, urine contains products of its metabolism or disintegration.…”

Section: Original Articlementioning

confidence: 99%

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Logoyda¹

2019

IJGP

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Introduction: Coaxil (tianeptine) is a new generation antidepressant with selective serotoninergic action. Recently, a significant increase in non-medical use of the atypical tricyclic antidepressant drug, tianeptine, has been observed in Ukraine. According to domestic narcologists and psychiatrists, there is information on tianeptine abuse by drug addicts. In literature, there is information that this medicine combines characteristics of opium, heroin, and cocaine in it. Receiving of tianeptine in high dosages (400-600 mg intravenously) leads to a state of euphoria and gives a narcotic effect that is several times stronger than heroin and cocaine. The aim of the Study: The aim of the research was to study of tianeptine metabolism products in the human body in urine by gas chromatography-mass spectrometry. Methods: The analysis has been carried out on a gas chromatograph Agilent 6890N with a massselective detector Agilent 5973 and an injector Agilent 7683 (USA) equipped with a chromatographic quartz capillary geyser of FactorFour Varian (USA). Gas carrier was helium. Results and Discussion: The analysis of extracts received as with acidic and with alkaline pH values has shown metabolites of tianeptine MC5 in them. Besides the main metabolite (MC5), there have also been desalkyldesaminotianeptine and desalkyldesaminonortianeptine in the extracts obtained at pH 3-4 and in the extracts obtained at pH 9-10-desalkyltianeptine and a metabolite, the structure of which has not been established at the moment. Based on the fact that the main metabolite of tianeptine MC5 is isolated from the urine both at acidic pH values (pH = 3-4) and alkaline (pH = 9-10), it is possible to use as acidic as alkaline extracts for the chromatography-mass spectrometry analysis. Conclusions: The possibility of using gas chromatography and mass spectrometry methods for metabolites has been shown. The main metabolite of tianeptine can be isolated from the urine at acidic and also at alkaline pH values of the medium.

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“…Dihydropyrroles may be employed as important synthetic intermediates for synthesis of pyrroles. Substituted dihydropyrroles are a vital class of five membered nitrogen-containing heterocycles as they are present in numerous bioactive compounds and pharmaceuticals, such as sibiromycin [28], anthramycin [29], serotonin reuptake inhibitor [30], and thienamycin [31]. Furthermore, dihydropyrroles can be used as versatile synthetic intermediates for the synthesis of natural products [32].…”

Section: Introductionmentioning

confidence: 99%

In Silico Approach Towards the Prediction of Drug-likeness, in Vitro Microbial Investigation and Formation of Dihydropyrrolone Conjugates

Alizadeh

Nazari

2020

Adv. J. Chem. A

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In this research study, we have synthesized a library of 2-substituted-1-(2-(5-((5-5-benzoyl-1H-benzo[d][1,2,3]triazole-1-yl)methyl)-2-thioxo-1,3,4-oxadiazol-3(2H)-yl) acetamido)-5-oxo-2,5-dihydro-1H-pyrrole-3carboxylic acids from 1H-benzo[d][1,2,3] triazole-5-yl)(phenyl)methanone. The synthesized compounds were characterized using 1 H NMR, 13 C NMR, C,H,N elemental analysis and mass spectroscopy studies. All the compounds were investigated for their in silico ADME prediction properties, in vitro antibacterial activity against four bacterial strains, antifungal activity against two fungal strains, and antimycobacterial activity against the H37Rv. All the compounds revealed good to moderate activity against the bacterial strain. Among all the compounds, 6b and 6f showed better antimycobacterial agents compared with that of the standard drug ciprofloxacin and pyrazinamide, whereas 6a, 6b, and 6e were found to be excellent antifungal and antibacterial agent compared standard drugs clotrimazole and ciprofloxacin. The results of the in-silico analysis depicted that the synthesized compounds had excellent drug-likeness properties.

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Development of High-Performance Liquid Chromatography Method for the Simultaneous Analysis of Amlodipine and Valsartan in Combined Dosage Form and in Vitro Dissolution Studied

Cited by 12 publications

References 6 publications

Efficient Validated Method of HPLC to Determine Enalapril in Combinated Dosage Form Containing Enalapril and Bisoprolol and in Vitro Dissolution Studies

Efficient Validated Method of HPLC to Determine Enalapril in Combinated Dosage Form Containing Enalapril and Bisoprolol and in Vitro Dissolution Studies

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In Silico Approach Towards the Prediction of Drug-likeness, in Vitro Microbial Investigation and Formation of Dihydropyrrolone Conjugates

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