Development of Immunonutrient from Pumpkin (Cucurbita Moschata Duchense Ex. Lamk.) Seed

Abstract

Pumpkin seeds has long been used as a source of nutrition. Based on its content, it is possible that part of this plant used as immunonutrient. The purpose of this research is to study the immunomodulatory activity of pumpkin seeds. It effects on the nonspecific immune response was determined through carbon clearance test, organ indexes (liver, spleen and thymic gland) and mice peritoneal exudate activity (PEA). The effects on specific immune response were determined through total antibody titre, delayed type of hypersensitivity (DTH) reaction and number of lymphocytes. On non specific immune response test, pumpkin seed at a low dose (PLD-3.8g/kg bw) and at a high dose (PHD-7.6g/kg bw) showed immunostimulanting effect with phagocytic index of 1.219 and 1.347, respectively. PHD increased PEA activity (p<0.01) in lysing microbes. On specific immune response test, PLD and PHD showed immunostimulation effect indicated through 50% haemaglutination activity (HA titre) i.e., 1:512 and 1:2048, respectively compared to than that of control (1:256) for secondary antibody titre. Through cellular immunity test, 48 hours after challenged, PHD showed significant (p<0.05) increased in footpad thickness. After 7 days administration, both doses of pumpkin increased lymphocytes number significantly (p<0.05). Based on the data it can be concluded that, pumpkin seeds have immunomodulatory effects, and can therefore be used as immunonutrient

The immunostimulant activity of Tibb an-Nabawi natural products: a literature review

“…Meningkatkan aktivitas splenosit dan makrofag Meningkatkan indeks fagositik, titer antibodi, dan jumlah sel limfosit (Kim et al, 2016); (Iwo et al, 2014) Proses studi literatur pertama diawali dengan pencarian informasi tentang berbagai bahan alam yang diketahui masuk kategori thibbun Nabawi, dengan ditunjang oleh Al-Quran dan atau Hadist.…”

“…Beta-karoten juga diketahui memiliki aktivitas peningkat sistem imun dengan meningkatkan aktivitas sel T. Selain ditunjukkan oleh daging buahnya, aktivitas imunostimulan juga ditunjukkan oleh bijinya. Pemberian serbuk biji labu terhadap hewan uji terbukti mampu meningkatkan respon imun non spesifik yang ditandai dengan peningkatan indeks fagositosis dan juga respon imun spesifik yang ditandai dengan peningkatan produksi antibodi dan sel limfosit (Iwo et al, 2014). Hasil tersebut menunjukkan bahwa labu kuning dan bijinya mampu mendukung respon imun selular dengan peningkatan aktivitas fagositik yang salah satunya diperantarai oleh makrofag juga mendukung respon imun selular dengan peningkatan produksi antibodi.…”

The optimization of ultrasonic method for the extraction of Pumpkin seed (Cucurbita maxima)

“…Pemanfaatan kandungan senyawa bioaktif biji labu kuning untuk pengobatan telah banyak dilaporkan, diantaranya sebagai antioksidan [1, 3,5], memperbaiki sistem urinaria pada manusia yang mengalami overactive bladder [8], memperbaiki postparsial glycemic [9], imunomudulator [10], menghambat sitotoksisitas dan genotosisitas [11], memicu pertumbuhan dan enzim proteolitik [12], menghambat sel kanker [13], antidiabetes [14].…”

Immunostimulant Activity of Marchantia paleacea Bertol. Herb Liverwort Ethanol Extract in BALB/c Mice

“…At present, many synthetic compounds and herbal products are used as immunomodulators in modulating nonspecific and specific immune responses. The use of medicinal plants with immunostimulant effects in patients reported less toxicity and side effects, thus attracting researchers to focus on exploring medicinal plants and their derivative compounds from plants that can improve certain immune responses (Iwo, et al, 2014;Kumolosasi et al, 2018).…”