Development of Injection Gas Heating System for Introducing Large Droplets to Inductively Coupled Plasma

Kaburaki, Yuki; Nomura, Akito; Ishihara, Yukiko; Iwai, Toshiaki; Miyahara, Hidekazu; Okino, Akitoshi

doi:10.2116/analsci.29.1147

Cited by 12 publications

(8 citation statements)

References 22 publications

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“…To irradiate living bodies with plasma, accurate temperature control is required to prevent thermal damage to the target. For example, to irradiate skin with plasma, the plasma gas temperature should be controlled such that it is below the denaturation temperature of proteins Tümmel et al, 2007 . The gas temperature of general atmospheric non-equilibrium plasma is lower than that of atmospheric equilibrium plasma, which is usually several thousand degrees Iwai et al, 2013;Kaburaki et al, 2013 . However, because plasma is generated from gas at room temperature by discharge, the gas temperature of the plasma bacteria after plasma irradiation were investigated.…”

Section: Introductionmentioning

confidence: 99%

Influence of Gas Temperature in Atmospheric Non-Equilibrium Plasma on Bactericidal Effect

et al. 2018

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In this study, the relationship between plasma gas temperature and the bactericidal effects on five of bacteria Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Enterococcus faecalis and Bacillus cereus spore in liquid was investigated using a temperature-controllable plasma source. We determined that the bactericidal ability improved as the plasma gas temperature increased. Specifically, the bactericidal ability on E. coli of 80-plasma was enhanced by as much as 6.3 times compared to that of 10-plasma. The relationship between plasma gas temperature and the amount of hydroxyl radical, singlet oxygen, hydrogen peroxide, and ozone introduced into the solution was investigated. Our results also showed that each reactive species production increased by 2.1, 9.0, 1.6, and 17 times, respectively, with 80-compared to 10plasma. The relationship between the bactericidal ability and amount of reactive species indicated that singlet oxygen and ozone introduced to the solution mostly influenced the bactericidal ability as the plasma gas temperature increased. We conclude that the plasma gas temperature is the crucial parameter for plasma sterilization.

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Section: Introductionmentioning

confidence: 99%

Influence of Gas Temperature in Atmospheric Non-Equilibrium Plasma on Bactericidal Effect

et al. 2018

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“…Non-thermal atmospheric-pressure plasmas have attracted attention in various fields such as the manufacturing and pharmaceutical industries and in environmental control [ 7 – 8 ]. Previously, we reported that a multi-gas plasma jet was able to cause surface hydrophilization of polyimide films [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Direct protein introduction into plant cells using a multi-gas plasma jet

et al. 2017

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Protein introduction into cells is more difficult in plants than in mammalian cells, although it was reported that protein introduction was successful in shoot apical meristem and leaves only together with a cell-penetrating peptide. In this study, we tried to introduce superfolder green fluorescent protein (sGFP)-fused to adenylate cyclase as a reporter protein without a cell-penetrating peptide into the cells of tobacco leaves by treatment with atmospheric non-thermal plasmas. For this purpose, CO2 or N2 plasma was generated using a multi-gas plasma jet. Confocal microscopy indicated that sGFP signals were observed inside of leaf cells after treatment with CO2 or N2 plasma without substantial damage. In addition, the amount of cyclic adenosine monophosphate (cAMP) formed by the catalytic enzyme adenylate cyclase, which requires cellular calmodulin for its activity, was significantly increased in leaves treated with CO2 or N2 plasma, also indicating the introduction of sGFP-fused adenylate cyclase into the cells. These results suggested that treatment with CO2 or N2 plasma could be a useful technique for protein introduction into plant tissues.

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“…13,14 In our research group, microdroplet injection was also developed to introduce single cells into an ICP. [15][16][17] In this system, the sample introduction efficiency equals 100%; in principle and the sample does not tend to diffuse in the plasma. The peak value of the signal increases because the sample is temporally and spatially compressed, producing a high signal-to-noise ratio.…”

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A pulse-synchronized microplasma atomic emission spectroscopy system for ultrasmall sample analysis

Iwai

Okumura

Kakegawa

et al. 2014

J. Anal. At. Spectrom.

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Regenerative medicine and environmental science require the analysis of ultrasmall samples such as cells and nanoparticles. Therefore, a microplasma atomic emission spectroscopy (AES) system was developed in this study. This system combined microdroplet injection, which can introduce a small droplet of a few tens of picoliters into a plasma excitation/ionization source, with a microhollow cathode plasma source exhibiting a volume of a few microliters. When a 14 pL droplet of 100 mg L À1 sodium solution was introduced into an 8 W DC microplasma, no emission was observed possibly because of an insufficient excitation of the sample at low plasma gas temperature. Therefore, a pulsed discharge, producing highintensity electric input power, was implemented to give a pulse-synchronized microplasma AES system.A 15 ms pulsed power reaching a maximum value of 100 kW was obtained using a laboratory-built highintensity pulsed power supply and was applied to generate the plasma. This system achieved an excitation temperature of 7000 K, exceeding that of the common inductively coupled plasma. Pulsed plasma generation and sample droplet introduction into the plasma were synchronized to provide a high-sensitivity AES analysis. To this end, the time interval before the end of the generation and the beginning of the pulsed plasma generation was adjusted using a delay circuit. The optimal delay amounted to 40 ms. A droplet comprising Na, Ca, Mg, and K at 100 mg L À1 was analyzed using this system and the limits of detection equaled 300, 50, 30, and 640 fg for these analytes, respectively.

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Development of Injection Gas Heating System for Introducing Large Droplets to Inductively Coupled Plasma

Cited by 12 publications

References 22 publications

Influence of Gas Temperature in Atmospheric Non-Equilibrium Plasma on Bactericidal Effect

Influence of Gas Temperature in Atmospheric Non-Equilibrium Plasma on Bactericidal Effect

Direct protein introduction into plant cells using a multi-gas plasma jet

A pulse-synchronized microplasma atomic emission spectroscopy system for ultrasmall sample analysis

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