Development of microfluidic devices for biomedical and clinical application

Webster, Abigail; Greenman, John; Haswell, Stephen J.

doi:10.1002/jctb.2482

Cited by 51 publications

(37 citation statements)

References 66 publications

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“…Mimicking the action of capillaries in microchannels and the use of components such as polydimethyl siloxane (PDMS) that are permeable to diffusion of oxygen are essential for cell survival in long-term 3D cell cultures necessary in toxicology studies. Microfluidic platforms integrate a plethora of other substrates to allow flexibility in use such as glass, paper, natural ECM compounds and agarose (Xia et al, 1998; Jang et al, 2008; Derda et al, 2009; Webster et al, 2011). Importantly, synthetic and natural substrates provide different stiffness degrees that contribute to determining the response to microenvironmental stimuli, notably via an impact on the epigenome (Qin et al, 2010; Johnstone et al, 2010; Du et al, 2016).…”

Section: Importance Of Tissue Architecture For Carcinogenesismentioning

confidence: 99%

Architecture in 3D cell culture: An essential feature for in vitro toxicology

Lelièvre

Kwok

Chittiboyina

2017

Toxicology in Vitro

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Three-dimensional cell culture has the potential to revolutionize toxicology studies by allowing human-based reproduction of essential elements of organs. Beyond the study of toxicants on the most susceptible organs such as liver, kidney, skin, lung, gastrointestinal tract, testis, heart and brain, carcinogenesis research will also greatly benefit from 3D cell culture models representing any normal tissue. No tissue function can be suitably reproduced without the appropriate tissue architecture whether mimicking acini, ducts or tubes, sheets of cells or more complex cellular organizations like hepatic cords. In this review, we illustrate the fundamental characteristics of polarity that is an essential architectural feature of organs for which different 3D cell culture models are available for toxicology studies in vitro. The value of tissue polarity for the development of more accurate carcinogenesis studies is also exemplified, and the concept of using extracellular gradients of gaseous or chemical substances produced with microfluidics in 3D cell culture is discussed. Indeed such gradients-on-a-chip might bring unprecedented information to better determine permissible exposure levels. Finally, the impact of tissue architecture, established via cell-matrix interactions, on the cell nucleus is emphasized in light of the importance in toxicology of morphological and epigenetic alterations of this organelle.

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Section: Importance Of Tissue Architecture For Carcinogenesismentioning

confidence: 99%

Architecture in 3D cell culture: An essential feature for in vitro toxicology

Lelièvre

Kwok

Chittiboyina

2017

Toxicology in Vitro

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“…Este trabalho não busca fazer uma revisão da literatura científica sobre este assunto, uma vez que várias revisões têm sido publicadas recentemente. [1][2][3][4][5][6][7][8][9][10][11] O seu objetivo é apresentar o que são estes micro reatores e quais são as características que o potencializam como ferramenta de inovação numa ampliação de escala.…”

Section: Introductionunclassified

Microreactors: New Opportunities in Chemical Synthesis

Machado¹,

Pandoli²,

Miranda³

et al. 2014

Revista Virtual De Química

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Abstract:The implementation of chemical reactions in continuous mode under microfluidic conditions by the use of microreactors is a very attractive alternative to the traditional batch and semi-batch mode currently used for chemical synthesis. The fine control of the stoichiometry, kinetics and thermal exchange of the reaction, as well as the easy scale up, low physical space demand for installation/operation and the easy implementation of in-line analysis techniques for in process controls approach the laboratory development to a reproducible, robust, safe, cost-competitive and environmentally sustainable chemical process. ResumoA execução de reações químicas em modo contínuo de operação sob condições microfluídicas em micro reatores é uma alternativa muito atraente às condições em batelada e semi batelada tradicionalmente empregadas para a síntese química. O fino controle da estequiometria, cinética e troca térmica da reação em execução, bem como a facilidade na ampliação de escala de produção, baixa demanda por espaço físico para sua instalação/operação e viabilidade na implementação de técnicas de análise em linha para os controles em processo, aproximam o desenvolvimento laboratorial da implementação de um processo químico reprodutível, robusto, seguro, de custo competitivo e ambientalmente sustentável.Palavras-chave: Micro reatores; processos químicos; química em fluxo.

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“…[14][15][16][17] Aside from cost-and time-efficiency of microfluidic experiments, the appeal of this microscale technology comes from the ability to quickly generate precisely controlled, stable, and spatially varying mixtures of chemical reagents and biological samples. [18][19][20] Thus, microfluidic devices like the platform we present in this paper can offer precise control over the type and concentration of chemical cues in the cellular microenvironment. Such microfluidics-based concentration gradient devices can generally be separated into passive (diffusive) and active (convection based) gradient generators (GG).…”

Section: Introductionmentioning

confidence: 99%

Polyester μ-assay chip for stem cell studies

et al. 2012

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The application of microfluidic technologies to stem cell research is of great interest to biologists and bioengineers. This is chiefly due to the intricate ability to control the cellular environment, the reduction of reagent volume, experimentation time and cost, and the high-throughput screening capabilities of microscale devices. Despite this importance, a simple-to-use microfluidic platform for studying the effects of growth factors on stem cell differentiation has not yet emerged. With this consideration, we have designed and characterized a microfluidic device that is easy to fabricate and operate, yet contains several functional elements. Our device is a simple polyester-based microfluidic chip capable of simultaneously screening multiple independent stem cell culture conditions. Generated by laser ablation and stacking of multiple layers of polyester film, this device integrates a 10 Â 10 microwell array for cell culture with a continuous perfusion system and a non-linear concentration gradient generator. We performed numerical calculations to predict the gradient formation and calculate the shear stress acting on the cells inside the device. The device operation was validated by culturing murine embryonic stem cells inside the microwells for 5 days. Furthermore, we showed the ability to maintain the pluripotency of stem cell aggregates in response to concentrations of leukemia inhibitory factor ranging from 0 to $1000 U/ml. Given its simplicity, fast manufacturing method, scalability, and the cell-compatible nature of the device, it may be a useful platform for long-term stem cell culture and studies.

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Development of microfluidic devices for biomedical and clinical application

Cited by 51 publications

References 66 publications

Architecture in 3D cell culture: An essential feature for in vitro toxicology

Architecture in 3D cell culture: An essential feature for in vitro toxicology

Microreactors: New Opportunities in Chemical Synthesis

Polyester μ-assay chip for stem cell studies

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