Development of novel agar media for isolating guaiacol producing Alicyclobacillus spp.

Crescimento e esporulação de Alicyclobacillus Acidoterrestris em meio de cultura e em suco de laranja industrializado

“…On the other hand, at a concentration of 100 mg/L the same was not observed. A result consistent with that found by Chang et al (2013) when they evaluated the growth of A.…”

“…Regarding the tests carried out to determine guaiacol through the reaction with the dye Cromoazurol S., it was not possible to observe a change in the coloring of the BAT culture medium when adding the dye, and it is not possible to identify a visual change resulting from the reaction of the dye with the guaiacol produced by the microorganism, as shown in Figure 2. Chang et al (2013) were able to identify the production of guaiacol by adding the dye Cromoazurol S. in the SK2 culture medium, however, the objective of the present study would be to evaluate the action of this dye in BAT medium, since it is a widely used medium.…”

“…In order to verify the qualitative formation of guaiacol, added media were prepared with the dye chromoazurol S. in the concentrations of 10 and 100 mg/L of BAT culture medium, values established according to the methodology used by Chang (2013). Tubes containing 3 mL of BAT medium were supplemented with 0.3 and 3 mg of the dye and added with bacterial suspensions of Alicyclobacillus acidoterrestris previously incubated at 45 °C for 24 hours and diluted to a concentration of 10 4 CFU/mL and then incubated at temperature and time determined by Chang (2013).…”

“…In order to verify the effect of vanillic acid on the growth of the microorganism, the culture medium (BAT) was enriched with vanillic acid in concentrations of 10 and 100 mg/L, values established according to the methodology used by Chang (2013). Tubes containing 3 mL of BAT medium were supplemented with 0.3 mg and 3 mg of vanillic acid and added with bacterial suspensions of Alicyclobacillus acidoterrestris previously incubated at 45 °C for 24…”

“…hours and diluted to a concentration of 10 4 CFU/mL and then incubated in the temperature and time determined by Chang (2013).…”