Aim:The goal of this study is to find out if Mito-TEMPO, an antioxidant that targets mitochondria, can improve the quality of frozen human sperm.Material-Method: Normospermic 25 human semen samples were frozen and stored in solutions including different concentrations (0, 5, 10 and 50 µM) of Mito-TEMPO. After thawing, they were evaluated in terms of sperm motility, viability, morphology, chromatin integrity and apoptosis.Result: Sperm morphology was not found to change after cryopreservation. Viability was found to be significantly preserved in groups that were added Mito-TEMPO (p<0.01). When the motility of the sperms after they had been frozen was compared, the group that added 50 µM Mito-TEMPO had the most motile sperms. (p<0.05), even though there was no statistically significant difference between the groups that added 1 µM Mito-TEMPO and the groups that did not add Mito-TEMPO. In addition, chromatin decondensation and apoptosis rate decreased significantly in groups that were added Mito-TEMPO (p<0.01).
Conclusion:Mitochondria targeted antioxidant Mito-TEMPO improves sperm quality that decreases after thawing.