Development of specific and degenerate primers for CesA genes encoding cellulose synthase in flax (Linum usitatissimum L.)

Grushetskaya, Z. E.; Лемеш, В. А.; Хотылева, Л. В.

doi:10.3103/s0095452710040018

Cited by 4 publications

(3 citation statements)

References 14 publications

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“…In the flax genome, 16 predicted LusCESAs were identified ( Table 3 ). Previously only partial sequences of some flax CESAs were published [43] . All 16 flax CESAs could be placed in discrete clades with Arabidopsis and Populus CESA homologs ( Figure 5 ).…”

Section: Discussionmentioning

confidence: 99%

Chitinase-Like (CTL) and Cellulose Synthase (CESA) Gene Expression in Gelatinous-Type Cellulosic Walls of Flax (Linum usitatissimum L.) Bast Fibers

2014

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Plant chitinases (EC 3.2.1.14) and chitinase-like (CTL) proteins have diverse functions including cell wall biosynthesis and disease resistance. We analyzed the expression of 34 chitinase and chitinase-like genes of flax (collectively referred to as LusCTLs), belonging to glycoside hydrolase family 19 (GH19). Analysis of the transcript expression patterns of LusCTLs in the stem and other tissues identified three transcripts (LusCTL19, LusCTL20, LusCTL21) that were highly enriched in developing bast fibers, which form cellulose-rich gelatinous-type cell walls. The same three genes had low relative expression in tissues with primary cell walls and in xylem, which forms a xylan type of secondary cell wall. Phylogenetic analysis of the LusCTLs identified a flax-specific sub-group that was not represented in any of other genomes queried. To provide further context for the gene expression analysis, we also conducted phylogenetic and expression analysis of the cellulose synthase (CESA) family genes of flax, and found that expression of secondary wall-type LusCESAs (LusCESA4, LusCESA7 and LusCESA8) was correlated with the expression of two LusCTLs (LusCTL1, LusCTL2) that were the most highly enriched in xylem. The expression of LusCTL19, LusCTL20, and LusCTL21 was not correlated with that of any CESA subgroup. These results defined a distinct type of CTLs that may have novel functions specific to the development of the gelatinous (G-type) cellulosic walls.

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Section: Discussionmentioning

confidence: 99%

Chitinase-Like (CTL) and Cellulose Synthase (CESA) Gene Expression in Gelatinous-Type Cellulosic Walls of Flax (Linum usitatissimum L.) Bast Fibers

2014

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“…Fragments of the conserved domain B of the CesA -6 subunit (GenBank: KF011584.1) were obtained from cDNA of L. usitatissimum by PCR with primers F_À6_302: 5 ′ -TTATTGCTGTCCAGAGAGAG-3 ′ and R_A6_302: 5′-AGAACCATATACTGGCAAGA-3′ developed previously ( Grushetskaya et al, 2010 ). These DNA fragments were cloned using pGEM-T Easy Vector System (Promega, Madison, WI, United States) in competent Escherichia coli cells of DHS a strain and isolated by the Plasmid DNA Isolation kit (Evrogen, Moscow, Russian Federation) according to the manufacturer’s protocols.…”

Section: Methodsmentioning

confidence: 99%

“…The CesA genes can be identified by the P-CR sequence which is highly conserved in all plant CesA s ( Doblin et al, 2002 ; Kumar and Turner, 2015 ; Kaur et al, 2016 ). Based on comparative studies of the nucleotide sequences of six flax CesA EST subunits (GenBank: EF409998–EF410000, EF214742–EF214744), the primers amplifying the 301 bp cDNA fragment of CesA -6 subunit (GenBank: KF011584.1) were designed ( Grushetskaya et al, 2010 ). The alignment of the nucleotide sequence of the amplified CesA -6 fragment with the known sequences of the CesA genes of Arabidopsis, Populus , and Eucalyptus showed that the obtained KF011584.1 fragment comprised the region of the conserved P-CR2 domain of the CesA genes ( Richmond and Somerville, 2000 ; Liang and Joshi, 2004 ; Ranik and Myburg, 2006 ).…”

Section: Introductionmentioning

confidence: 99%

Integration of Physical, Genetic, and Cytogenetic Mapping Data for Cellulose Synthase (CesA) Genes in Flax (Linum usitatissimum L.)

et al. 2017

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Flax, Linum usitatissimum L., is a valuable multi-purpose plant, and currently, its genome is being extensively investigated. Nevertheless, mapping of genes in flax genome is still remaining a challenging task. The cellulose synthase (CesA) multigene family involving in the process of cellulose synthesis is especially important for metabolism of this fiber crop. For the first time, fluorescent in situ hybridization (FISH)-based chromosomal localization of the CesA conserved fragment (KF011584.1), 5S, and 26S rRNA genes was performed in landrace, oilseed, and fiber varieties of L. usitatissimum. Intraspecific polymorphism in chromosomal distribution of KF011584.1 and 5S DNA loci was revealed, and the generalized chromosome ideogram was constructed. Using BLAST analysis, available data on physical/genetic mapping and also whole-genome sequencing of flax, localization of KF011584.1, 45S, and 5S rRNA sequences on genomic scaffolds, and their anchoring to the genetic map were conducted. The alignment of the results of FISH and BLAST analyses indicated that KF011584.1 fragment revealed on chromosome 3 could be anchored to linkage group (LG) 11. The common LG for 45S and 5S rDNA was not found probably due to the polymorphic localization of 5S rDNA on chromosome 1. Our findings indicate the complexity of integration of physical, genetic, and cytogenetic mapping data for multicopy gene families in plants. Nevertheless, the obtained results can be useful for future progress in constructing of integrated physical/genetic/cytological maps in L. usitatissimum which are essential for flax breeding.

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Cellulose synthase genes that control the fiber formation of flax (Linum usitatissimum L.)

et al. 2014

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Development of specific and degenerate primers for CesA genes encoding cellulose synthase in flax (Linum usitatissimum L.)

Cited by 4 publications

References 14 publications

Chitinase-Like (CTL) and Cellulose Synthase (CESA) Gene Expression in Gelatinous-Type Cellulosic Walls of Flax (Linum usitatissimum L.) Bast Fibers

Chitinase-Like (CTL) and Cellulose Synthase (CESA) Gene Expression in Gelatinous-Type Cellulosic Walls of Flax (Linum usitatissimum L.) Bast Fibers

Integration of Physical, Genetic, and Cytogenetic Mapping Data for Cellulose Synthase (CesA) Genes in Flax (Linum usitatissimum L.)

Cellulose synthase genes that control the fiber formation of flax (Linum usitatissimum L.)

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