Development of transgenic chickens expressing enhanced green fluorescent protein

Kwon, Mo Sun; Koo, Bon Chul; Choi, Bok Ruyl; Lee, Hoon Taek; Kim, Young Hye; Ryu, Wang Shick; Shim, Hosup; Kim, Jin‐Hoi; Kim, Nam Hyung; Kim, Teoan

doi:10.1016/j.bbrc.2004.05.197

Cited by 37 publications

(22 citation statements)

References 30 publications

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“…Oncogenic viruses have shown promise (Ishii et al, 2004), including avian leucosis virus (ALV) and reticuloendotheliosis virus (REV) (Salter et al, 1986;Salter et al, 1987;Bosselman et al, 1989a;Bosselman et al, 1989b), and Moloney murine leukemia virus (MoMLV) (Mizuarai et al, 2001). A newly reported MoMLVtransduced chick, utilizing the vesicular stomatitis virus G glycoprotein (VSV-G) vector pseudotyping system, expresses GFP under control of the rous sarcoma virus (RSV) promoter (Kwon et al, 2004). G0 birds produced using this system are chimeric, but G1 analysis has yet to be reported.…”

Section: Introductionmentioning

confidence: 99%

Ubiquitous GFP expression in transgenic chickens using a lentiviral vector

Chapman

Lawson

MacArthur³

et al. 2005

Development

124

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We report the first ubiquitous green fluorescent protein expression in chicks using a lentiviral vector approach, with eGFP under the control of the phosphoglycerol kinase promoter. Several demonstrations of germline transmission in chicks have been reported previously, using markers that produce tissue-specific, but not ubiquitous, expression. Using embryos sired by a heterozygous male, we demonstrate germline transmission in the embryonic tissue that expresses eGFP uniformly, and that can be used in tissue transplants and processed by in situ hybridization and immunocytochemistry. Transgenic tissue is identifiable by both fluorescence microscopy and immunolabeling, resulting in a permanent marker identifying transgenic cells following processing of the tissue. Stable integration of the transgene has allowed breeding of homozygous males and females that will be used to produce transgenic embryos in 100% of eggs laid upon reaching sexual maturity. These results demonstrate that a transgenic approach in the chick model system is viable and useful even though a relatively long generation time is required. The transgenic chick model will benefit studies on embryonic development, as well as providing the pharmaceutical industry with an economical bioreactor.

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Section: Introductionmentioning

confidence: 99%

Ubiquitous GFP expression in transgenic chickens using a lentiviral vector

Chapman

Lawson

MacArthur³

et al. 2005

Development

124

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“…야생형 녹색형광 단백질(wtGFP)의 아미노산을 점 돌연변이 시켜 만들어진 EGFP (enhanced green fluorescent protein) 단백질은 야생형에 비해 높은 강도를 나타내는 녹색형광 단백질로 (Cinelli et al, 2000), 세포 및 분자생물학 분야에서 녹색형광 발현 형질전환체를 제작하거나 형 질전환체 선별을 위한 선발마커 등에 흔히 이용되고 있으며 (Arun et al, 2005, Joo andWang, 2008), 우리나라에 서도 GFP 발현 쥐와 닭 모델이 개발 되어 있다 (Sim et al, 2009, Kwon et al, 2004 (Lee and Kim, 2000). 최초의 형질전환누에가 개발된 이래 형질전환 누에에서 기능성 단백질을 생산하기 위한 연구가 활발히 진행되고 있으며, 이 중 인공 실크 생산을 위한 곤충으로 서의 이용가능성이 대두되고 있다 (Imamura et al, 2003, Prudhomme and Couble, 2002, Satoshi et al 2007, Tamura et al, 2000.…”

Section: Introductionunclassified

Single-dose oral toxicity study of genetically modified silkworm expressing EGFP protein in ICR mouse

Jang¹,

Kim²,

Park³

et al. 2016

Korean Journal of Agricultural Science

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Silk has had a reputation as a luxurious and sensuous fabric but it is not popular due to the expensive price and poor durability. To develop the silk materials that apply the various industries, the artificially synthesized gene can be introduced into the silkworm and expressed in the silk gland. Transgenic silkworms for the mass production of green fluorescent silks are generated using a fibroin H-chain expression system. For commercial use, safety assessment of the transgenic silkworms is essential. The purpose of this study was to examine the potential acute oral toxicity of EGFP protein expressed in genetically modified (GM) fluorescence silkworm and to obtain the approximative lethal dose in the male and female at 6-weeks ICR mice. EGFP protein was fed at a dose of 2,000 mg/kg body weight in five male or five female mice. Mortalities, clinical findings and body weight changes were monitored for 1, 3, 7, 14 days after dosing. At the end of 14 day observation period, all mice were sacrificed, and the postmortem necropsy were performed. The test group was not observed death case. Also the effect was not admitted by test substance administration in common symptoms, the body weight and postmortem. The results of single-dose oral toxicity test showed that approximative lethal dose of EGFP protein expressed in fluorescence silkworm was considered to exceed the 2,000 mg/kg body weight in both sexes.

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“…Importantly, the ex vivo chick system provides a model without an immune system in early development EL Smith et al Chick limb organ culture in the 21 st century making it attractive for tissue/cell xenotransplantation (Boulland et al, 2010;Goldstein, 2006;Wichterle et al, 2008). It is also possible to utilise transgenesis techniques in embryos, one particular example being the development of green fluorescent protein (GFP)-expressing transgenic chicks (Kwon et al, 2004;McGrew et al, 2004;Sang, 2004). As such, the avian model system has made significant scientific contributions, not just to the field of developmental biology, but also to immunology, genetics, virology, cancer, cell biology and tissue engineering, as reviewed by Stern (2005).…”

Section: Introductionmentioning

confidence: 99%

A new take on an old story: chick limb organ culture for skeletal niche development and regenerative medicine evaluation

Smith

Kanczler²,

Oreffo³

2013

eCM

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Scientific research and progress, particularly in the drug discovery and regenerative medicine fields, is typically dependent on suitable animal models to develop new and improved clinical therapies for injuries and diseases. In vivo model systems are frequently utilised, but these models are expensive, highly complex and pose a number of ethical considerations leading to the development and use of a number of alternative ex vivo model systems. The ex vivo embryonic chick long bone and limb bud models have been utilised in the scientific research field as a model to understand skeletal development for over eighty years. The rapid development of avian skeletal tissues, coupled with the ease of experimental manipulation, availability of genome sequence and the presence of multiple cell and tissue types has seen such model systems gain significant research interest in the last few years in the tissue engineering field. The models have been explored both as systems for understanding the developmental bone niche and as potential testing tools for tissue engineering strategies for bone repair and regeneration. This review details the evolution of the chick limb organ culture system and presents recent innovative developments and emerging techniques and technologies applied to these models that are aiding our understanding of skeletal developmental and regenerative medicine research and application.

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Development of transgenic chickens expressing enhanced green fluorescent protein

Cited by 37 publications

References 30 publications

Ubiquitous GFP expression in transgenic chickens using a lentiviral vector

Ubiquitous GFP expression in transgenic chickens using a lentiviral vector

Single-dose oral toxicity study of genetically modified silkworm expressing EGFP protein in ICR mouse

A new take on an old story: chick limb organ culture for skeletal niche development and regenerative medicine evaluation

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