Development of up-converting phosphor technology-based lateral flow assay for quantitative detection of serum PIVKA-II: Inception of a near-patient PIVKA-II detection tool

Huang, Yi; Zhang, Songgao; Zheng, Qingzhu; Li, Yadong; Yu, Lili; Wu, Qingzhong; Zheng, Jiaying; Wu, Yanan; Qiu, Funan; Gao, Qi; Zhang, Jiancheng

doi:10.1016/j.cca.2018.11.020

Cited by 11 publications

(6 citation statements)

References 35 publications

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“…As aforementioned, LFAs are used in a variety of applications with samples that present extremely different composition and characteristics (Table 1). 27 For example, clinical samples can be whole blood, 28,29 plasma, 30,31 serum, [32][33][34] sweat, 35,36 urine, 37,38 stool, 39,40 saliva, 41,42 cerebrospinal fluid 43,44 and nasal swabs, 45,46 while food matrices could be juices, 47,48 cereals, 49,50 meat, 51,52 vegetables, 53 and environmental (mostly water and soil) samples. [54][55][56][57] Although the sample pad provides a means to control the properties of the sample solution (see the following section), some complex matrixes may require pre-treatment before an aliquot can be added into the LFA strip.…”

Section: Types Of Samples and Target Analytesmentioning

confidence: 99%

Tutorial: design and fabrication of nanoparticle-based lateral-flow immunoassays

et al. 2020

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Lateral flow assays (LFA) are quick, simple and cheap assays to analyse a variety of samples at the point of care or in the field, making them one of the most widespread biosensors currently available. They have been successfully employed for the detection of a myriad of different targets (ranging from atoms up to whole cells) in all type of samples (including water, blood, foodstuff and environmental samples). Their operation relies on the capillary flow of the sample within a series of sequential pads with different functionalities aiming to generate a signal indicating the absence/presence (and, in some cases, the concentration) of the analyte of interest. In order to have a user-friendly operation, their development requires the optimization of multiple, interconnected parameters that may overwhelm new developers. In this Tutorial we provide the readers with: 1) the basic knowledge to understand the principles governing an LFA and to take informed decisions during lateral flow strip design and fabrication, 2) a roadmap for optimal LFA development independent of the specific application, 3) a step by step example protocol for the assembly and operation of an LF strip for the detection of Human Immunoglobulin G and 4) an extensive troubleshooting section addressing the most frequent issues in designing, assembling and using LFAs.

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Section: Types Of Samples and Target Analytesmentioning

confidence: 99%

Tutorial: design and fabrication of nanoparticle-based lateral-flow immunoassays

et al. 2020

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“…In another study, an LFTS for monitoring chloramphenicol in milk was reported to have a quantitative detection limit 0.3 ng/mL by using a portable photometer . A number of LFTS based on up-converting phosphors have already been reported as providing greater sensitivity. − For example, an LFTS employing up-converting phosphors was developed for quantitative detection of serum PIVKA-II, using an up-converting phosphor immunoassay analyzer. The limit of detection for the assay was reported to be 2.66 ng/mL with a linear range 4.8–20,000 ng/mL .…”

Section: Lateral Flow Test Detection Methodsmentioning

confidence: 99%

“…A number of LFTS based on up-converting phosphors have already been reported as providing greater sensitivity. − For example, an LFTS employing up-converting phosphors was developed for quantitative detection of serum PIVKA-II, using an up-converting phosphor immunoassay analyzer. The limit of detection for the assay was reported to be 2.66 ng/mL with a linear range 4.8–20,000 ng/mL . In addition, a highly sensitive and accurate LFTS for investigating two highly addictive drugs, morphine and methamphetamine, was reported to have a limit of quantification of 5 and 10 ng/mL, respectively.…”

Section: Lateral Flow Test Detection Methodsmentioning

confidence: 99%

Nanopaper Biosensors at Point of Care

et al. 2022

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In this review, we provide an overview of the nanopaper biosensors used for point of care (POC) diagnostics in various fields such as biomedical, environmental, food safety, and agriculture. The lateral flow assays (LFAs) comprising nanoparticles have drawn the interest of researchers due to their high sensitivity, low cost, lower time consumption, lack of equipment, and easy handling characteristics. These assays have become an integral part of the health sector all over the world over a short time period and are extensively being engaged in diagnosis of viral infections in regions low on resources. A large number of innovative approaches have been introduced in making these test-strips or nanopaper biosensors as quickly as possible, because of their ease of operation, cheaper cost, and quick results, even with the simplest setups or in pathological laboratories, etc. Keeping all this in mind, we have reviewed the nanoparticles based lateral flow test strips (LFTS), their composition, working methods, reaction mechanisms, detection methods, and applications in different fields. We also provide an overview of paper-based microfluidic analytical devices (μPADs) and our perspective about the future trends which may facilitate understanding their applications.

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“…This process makes the particles suitable for biological applications. Using ethyl orthosilicate (TEOS), the surface of the UCP particles can be silicified through a series of chemical reactions, resulting in a large number of surface-active groups ( Huang Y. et al, 2019 ). Through these free active groups, UCP particles can be covalently combined with antigens, antibodies, nucleic acids, biotin, and other bioactive molecules to make them biologically active.…”

Section: Methodsmentioning

confidence: 99%

Rapid Detection of Cysticercus cellulosae by an Up-Converting Phosphor Technology-Based Lateral-Flow Assay

Zhang

Cui

et al. 2021

Front. Cell. Infect. Microbiol.

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Cysticercosis is a neglected tropical disease caused by the larvae of Taenia solium in pigs and humans. The current diagnosis of porcine cysticercosis is difficult, and traditional pathological tests cannot meet the needs of detection. This study established a UPT-LF assay for the detection of Cysticercus cellulosae. UCP particles were bound to two antigens, TSOL18 and GP50; samples were captured, and the signal from the UCP particles was converted into a detectable signal for analysis using a biosensor. Compared to ELISA, UPT-LF has higher sensitivity and specificity, with a sensitivity of 93.59% and 97.44%, respectively, in the case of TSOL18 and GP50 antigens and a specificity of 100% for both. Given its rapidness, small volume, high sensitivity and specificity, and good stability and reproducibility, this method could be used in the diagnosis of cysticercosis.

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Development of up-converting phosphor technology-based lateral flow assay for quantitative detection of serum PIVKA-II: Inception of a near-patient PIVKA-II detection tool

Cited by 11 publications

References 35 publications

Tutorial: design and fabrication of nanoparticle-based lateral-flow immunoassays

Tutorial: design and fabrication of nanoparticle-based lateral-flow immunoassays

Nanopaper Biosensors at Point of Care

Rapid Detection of Cysticercus cellulosae by an Up-Converting Phosphor Technology-Based Lateral-Flow Assay

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