Development, Preparation, and Biomedical Applications of DNA-Based Hydrogels

Jian, Xueting; Luo, Yuning; Li, Fangjie; Tan, Junyan; Yin, Yuli; Liu, Yang

doi:10.3389/fbioe.2021.661409

Cited by 26 publications

(14 citation statements)

References 74 publications

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“…Hydrogels and water-soluble gel networks are currently being extensively studied for their use as biocompatible materials. Hydrogels formed with DNA are largely reported in the literature. , Usually, these hydrogels are formed by hybridization of DNA Y-shaped structures or DNA-grafted polymers with a complementary DNA sequence leading to a network. In the current hydrogels, we showed that short sequences with only six units are able to form hydrogels by supramolecular arrangement strongly stabilized by CT of the DAN and NDI pairs.…”

Section: Discussionmentioning

confidence: 99%

Supramolecular Recognition of Phosphodiester-Based Donor and Acceptor Oligomers Forming Gels in Water

et al. 2023

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Inspired by automated DNA synthesis, electron-rich dialkoxynaphthalene (DAN) donor and electron-deficient naphthalene-tetracarboxylic diimide (NDI) acceptor phosphodiester-linked homohexamers were synthesized by the phosphoramidite method. Two types of hexamers were prepared, one with only one phosphodiester between the aromatics (i.e., DAN or NDI) and a second with two phosphodiesters around a propanediol between the aromatics, leading to the latter more flexible and more hydrophilic hexamers. The folding properties of these homohexamers alone or mixed together, in water only, were studied by UV–visible absorption spectroscopy and atomic force microscopy (AFM). AFM imaging revealed that a 1:1 mixture of hexaDAN and hexaNDI formed fibers by charge transfer donor–acceptor recognition leading to a hydrogel after drying. The organization of the resulting structures is strongly dependent on the nature of the complementary partner, leading to the formation of mono- or multilayer hydrogel networks with different compactness.

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Section: Discussionmentioning

confidence: 99%

Supramolecular Recognition of Phosphodiester-Based Donor and Acceptor Oligomers Forming Gels in Water

et al. 2023

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“…Hydrogel, a 3D polymer composite system consisting of water and cross-linked polymers, can imbibe large quantities of water or biological fluids ( Li et al, 2020b ; Jian et al, 2021 ). With its excellent stability, malleability, biocompatibility and sustained release, hydrogel is an ideal drug carrier for SCI treatment.…”

Section: Empirical Sci Treatment Involving Biomaterial-based Tlr4-nf-...mentioning

confidence: 99%

Strategies for Biomaterial-Based Spinal Cord Injury Repair via the TLR4-NF-κB Signaling Pathway

Shen

Cheng

et al. 2022

Front. Bioeng. Biotechnol.

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The repair and motor functional recovery after spinal cord injury (SCI) has remained a clinical challenge. Injury-induced gliosis and inflammation lead to a physical barrier and an extremely inhibitory microenvironment, which in turn hinders the recovery of SCI. TLR4-NF-κB is a classic implant-related innate immunomodulation signaling pathway and part of numerous biomaterial-based treatment strategies for SCI. Numerous experimental studies have demonstrated that the regulation of TLR4-NF-κB signaling pathway plays an important role in the alleviation of inflammatory responses, the modulation of autophagy, apoptosis and ferroptosis, and the enhancement of anti-oxidative effect post-SCI. An increasing number of novel biomaterials have been fabricated as scaffolds and carriers, loaded with phytochemicals and drugs, to inhibit the progression of SCI through regulation of TLR4-NF-κB. This review summarizes the empirical strategies for the recovery after SCI through individual or composite biomaterials that mediate the TLR4-NF-κB signaling pathway.

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“…According to the type of hydrogel cross-linking and components, DNA-based hydrogels can be divided into pure DNA-based hydrogels and hybrid DNA-based hydrogels. Pure DNA-based hydrogels are constructed solely using DNA molecules, which are assembled through Watson–Crick or non-Watson–Crick interactions, as well as enzymatic ligation or polymerization. , The latter is formed by connecting the DNA molecules that are grafted onto hydrophilic polymer chains, which function as interaction nodes. , In this review, we focus on the development of recent works on DNA-based hydrogels in bioanalysis and cancer therapeutics applications. Additionally, in order to promote the further development of DNA-based hydrogels, particularly in the field of biosensing, we also provide some insights into the current challenges and future prospects of these materials (Figure ).…”

Section: Introductionmentioning

confidence: 99%

Intelligent DNA-Based Hydrogels for Bioanalysis and Therapeutics

Zhu,

Shen,

Chen

et al. 2023

ACS Appl. Polym. Mater.

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DNA is a type of biomacromolecule that can be modified and designed through enzymatic or chemical techniques, and it serves as both a biological reactive entity and a polymer. In recent years, with the rapid development of chemistry, material science, and nanotechnology, researchers began to look at the DNA molecule from another perspective. The exceptional ability of DNA-based materials for selective recognition and structural design has attracted much attention. With the development of nanotechnology, there is an urgent need for smart hydrogel materials with excellent biocompatibility. Among many nanomaterials, DNA is considered to be an excellent smart material, which can spontaneously self-assemble into a precise and ordered structure under optimal conditions. Chemical bonding between DNA molecules or physical entanglement between DNA strands can be prepared from DNA-based smart hydrogels. Compared with traditional hydrogels, DNA-based hydrogels have the characteristics of functional DNA molecular programming and high-precision assembly, multifunctionality, and excellent biocompatibility and retain the excellent mechanical properties and physical properties of hydrogels. It has attracted increasing research interest in various fields, especially in biosensing and biomedical applications. In this review, we highlight the advances in DNA-based hydrogels for bioanalysis and therapeutics. We first summarize the construction methods and basic properties of DNA-based pure and hybrid hydrogels and discuss their research progress and biological applications. Subsequently, the practical applications of various types of DNA-based hydrogels in the fields of bioanalysis and drug delivery are described through some selected examples. Finally, the remaining challenges and prospects of DNA-based hydrogels are discussed in depth.

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Development, Preparation, and Biomedical Applications of DNA-Based Hydrogels

Cited by 26 publications

References 74 publications

Supramolecular Recognition of Phosphodiester-Based Donor and Acceptor Oligomers Forming Gels in Water

Supramolecular Recognition of Phosphodiester-Based Donor and Acceptor Oligomers Forming Gels in Water

Strategies for Biomaterial-Based Spinal Cord Injury Repair via the TLR4-NF-κB Signaling Pathway

Intelligent DNA-Based Hydrogels for Bioanalysis and Therapeutics

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