Developmental Changes in the Spatial Expression of Genes Involved in Myosin Function in Dictyostelium

Maeda, Mineko; Kuwayama, Hidekazu; Yokoyama, Minato; Nishio, Keiko; Morio, Takahiro; Urushihara, Hideko; Katoh, Masahiko; Tanaka, Yoshimasa; Saito, Tamao; Ochiai, Hiroshi; Takemoto, Kazuhiro; Yasukawa, Hiroo; Takeuchi, Ikuo

doi:10.1006/dbio.2000.9736

Cited by 21 publications

(20 citation statements)

References 29 publications

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“…The patterns of gene expression seen on microarrays are not significantly different for strains Ax2 and Ax4 (27). Our previous in situ studies were carried out with strain Ax2 (17,26), and we have continued to use this strain. After fixation, samples from each stage were mixed and hybridized in a single tube as described previously (8,17,26).…”

Section: Methodsmentioning

confidence: 99%

“…We have previously used this technique to characterize expression patterns of cytoskeletal and cyclic AMP signaling genes in prestalk cell subtypes (17,26). Using a combination of genome-wide discovery and in situ verification, we have now identified 3 genes enriched in PstA cells, 30 genes enriched in PstO cells, 10 genes enriched in PstAB cells, and 38 genes enriched in both PstA and -O cells.…”

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confidence: 99%

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Changing Patterns of Gene Expression in Dictyostelium Prestalk Cell Subtypes Recognized by In Situ Hybridization with Genes from Microarray Analyses

et al. 2003

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We used microarrays carrying most of the genes that are developmentally regulated in Dictyostelium to discover those that are preferentially expressed in prestalk cells. Prestalk cells are localized at the front of slugs and play crucial roles in morphogenesis and slug migration. Using whole-mount in situ hybridization, we were able to verify 104 prestalk genes. Three of these were found to be expressed only in cells at the very front of slugs, the PstA cell type. Another 10 genes were found to be expressed in the small number of cells that form a central core at the anterior, the PstAB cell type. The rest of the prestalk-specific genes are expressed in PstO cells, which are found immediately posterior to PstA cells but anterior to 80% of the slug that consists of prespore cells. Half of these are also expressed in PstA cells. At later stages of development, the patterns of expression of a considerable number of these prestalk genes changes significantly, allowing us to further subdivide them. Some are expressed at much higher levels during culmination, while others are repressed. These results demonstrate the extremely dynamic nature of cell-type-specific expression in Dictyostelium and further define the changing physiology of the cell types. One of the signals that affect gene expression in PstO cells is the hexaphenone DIF-1. We found that expression of about half of the PstO-specific genes were affected in a mutant that is unable to synthesize DIF-1, while the rest appeared to be DIF independent. These results indicate that differentiation of some aspects of PstO cells can occur in the absence of DIF-1.

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Section: Methodsmentioning

confidence: 99%

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Changing Patterns of Gene Expression in Dictyostelium Prestalk Cell Subtypes Recognized by In Situ Hybridization with Genes from Microarray Analyses

et al. 2003

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“…The thick filaments provide the cells with the force required to move through the mass of other cells to the cAMP signal coming from the centre of the aggregate (Maeda et al, 2000;Maeda et al, 2003;Verkerke-van Wijk et al, 2001) (Fig. 2B).…”

Section: Coordination Of Collective Movement During Dictyostelium Aggmentioning

confidence: 99%

Collective cell migration in development

Weijer

2009

Journal of Cell Science

290

230

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Collective cell migration is a key process during the development of most organisms. It can involve either the migration of closely packed mesenchymal cells that make dynamic contacts with frequently changing neighbour cells, or the migration of epithelial sheets that typically display more stable cell-cell interactions and less frequent changes in neighbours. These collective movements can be controlled by short- or long-range dynamic gradients of extracellular signalling molecules, depending on the number of cells involved and their distance of migration. These gradients are sensed by some or all of the migrating cells and translated into directed migration, which in many settings is further modulated by cell-contact-mediated attractive or repulsive interactions that result in contact-following or contact-inhibition of locomotion, respectively. Studies of collective migration of groups of epithelial cells during development indicate that, in some cases, only leader cells sense and migrate up an external signal gradient, and that adjacent cells follow through strong cell-cell contacts. In this Commentary, I review studies of collective cell migration of differently sized cell populations during the development of several model organisms, and discuss our current understanding of the molecular mechanisms that coordinate this migration.

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“…Whole-mount in situ hybridisation analyses were performed as described previously (Escalante and Loomis, 1995;Maeda et al, 2000;2003). In this study, Ax2 and Dd-STATa-null cells in an Ax2 background were allowed to develop on filter paper (FILTER PA-PER 4A, ADVANTEC, Tokyo, Japan) placed on nonnutrient agar at 22ºC.…”

Section: In Situ Hybridisationmentioning

confidence: 99%

Identification of new modes of Dd-STATa regulation of gene expression in Dictyostelium by in situ hybridisation

Shimada¹,

Maeda²,

Urushihara³

et al. 2004

Int. J. Dev. Biol.

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We identified 13 genes which are candidates for direct induction by Dd-STATa. In the parental strain, most of these genes are expressed in the cone shaped mass of pstAB cells which is located within the prestalk region. These cDNAs show little or no expression in the Dd-STATa-null strain. This contrasts markedly with the paradigmatic ecmB gene which is expressed in pstAB cells in parental cells, but which is expressed throughout the prestalk zone in the Dd-STATa-null strain. We also identified several genes which are normally expressed in pstA cells, or throughout the prestalk region, but whose expression is markedly down-regulated in the null mutant. Again, this contrasts with markers derived from the paradigmatic, ecmA gene which are expressed normally in the DdSTATa-null strain. The identification of these novel genes provides valuable tools to investigate the role of Dd-STATa.

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Developmental Changes in the Spatial Expression of Genes Involved in Myosin Function in Dictyostelium

Cited by 21 publications

References 29 publications

Changing Patterns of Gene Expression in Dictyostelium Prestalk Cell Subtypes Recognized by In Situ Hybridization with Genes from Microarray Analyses

Changing Patterns of Gene Expression in Dictyostelium Prestalk Cell Subtypes Recognized by In Situ Hybridization with Genes from Microarray Analyses

Collective cell migration in development

Identification of new modes of Dd-STATa regulation of gene expression in Dictyostelium by in situ hybridisation

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