Developmental competence of Dromedary camel (<i>Camelus dromedarius</i>) oocytes selected using brilliant cresyl blue staining

Fathi, Mohamed; Ashry, Mohamed; Salama, Asmaa; Badr, M. A.

doi:10.1017/s0967199417000387

Cited by 12 publications

(6 citation statements)

References 38 publications

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“…Of interest, the increase of the follicular diameter has been linked to the expansion of cells in the cumulus and to the in vitro maturation of bovine oocytes (KAUFFOLD et al, 2005). In our study, the cleavage rate followed a comparable pattern as the MII rate, and similar results were reported in other study (ALM et al, 2005;FATHI et al, 2017). Continuing along this line, we noted increased cleavage rates in the BCB+ oocytes of the large group, as compared to the small and BCB-free groups.…”

Section: Discussionsupporting

confidence: 90%

In vitro embryos production of oocytes selected based on follicular size and by brilliant cresyl blue staining

Barros

Adona

Guemra

et al. 2019

SCA

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We evaluated the competence of bovine oocytes via morphological selection associated with follicular diameter and by brilliant cresyl blue (BCB) staining, in order to improve oocyte selection and the efficiency of in vitro embryo production. Follicles with small (2 < 4 mm), large (4–8 mm) or mixed (2–8 mm) diameters were aspirated from slaughterhouse ovaries. Next, the morphologically categorized oocytes were stained and evaluated with 26 ?M of BCB (90 min), followed by an assessment of in vitro maturation (MII) and blastocyst production. Oocytes from the large (61.1 ± 3.9%) and mixed (52.9 ± 4.6%) follicular groups showed comparable (p > 0,05) BCB+ rates, but both differed (p < 0.05) as compared to the small-diameter group (41.0 ± 4.5%). Between the BCB+ and BCB – oocytes, there was no difference (p > 0,05) in MII rate in the mixed (respectively 86.7 ± 3.4 and 75.8 ± 2.2), large (respectively 89.4 ± 2.6 and 79.7 ± 2.3) and small (respectively 76.7 ± 2.9 and 67.2 ± 2.5) diameter groups. Comparing BCB (+ and -) oocytes, we observed (p > 0,05) no change in the percentage of embryos in the large group (respectively 67.3 ± 3.3 and 53.5 ± 5.8) and in the small group (respectively 43.8 ± 5.3 and 33.9 ± 2.2), but there was a difference (p < 0.05) in the mixed group (respectively 57.8 ± 3.9 and 39.6 ± 7.5). Furthermore, when we compared between follicular groups, the BCB+/large-diameter group (61.1 ± 3.9%) had a significant higher percentage of embryos (p < 0.05) than the small-diameter BCB+ (43.8 ± 5.3) groups. To conclude, selection procedures that use follicle size and/or BCB staining may be used as an additional tool to improve the in vitro production of embryos.

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Section: Discussionsupporting

confidence: 90%

In vitro embryos production of oocytes selected based on follicular size and by brilliant cresyl blue staining

Barros

Adona

Guemra

et al. 2019

SCA

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“…It is not surprising that the STAT3 transcript is the one of our top candidate genes highly expressed in competent oocytes. As we have shown in another study performed in our laboratory, this gene was also overexpressed in developmentally competent oocytes when selected based on BCB staining in bovine (Torner et al, 2008), and equine species (Mohammadi-Sangcheshmeh et al, 2011) and a study done in camel by an other group (Fathi et al, 2017). Activation of STAT3 induces cumulus expansion and oocyte maturation, while inhibition markedly prevented the cumulus expansion process (Tscherner et al, 2018).…”

Section: Association Of Odc1 and Stat3 Polymorphisms With Bull Fertilmentioning

confidence: 59%

Transcriptome profile and association study revealedSTAT3gene as a potential quality marker of bovine gametes

Ghanem

Salilew‐Wondim

Hoelker

et al. 2020

Zygote

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SummaryThe present study was aimed to investigate differences in molecular signatures in oocytes derived from Holstein-Friesian heifers with different genetic merit for fertility, euthanized during day 0 or day 12 of the estrous cycle. Moreover, association between single nucleotide polymorphisms (SNPs) of ODC1 and STAT3 genes and bull fertility traits was investigated. The gene expression patterns were analyzed using cDNA array and validated with quantitative real-time polymerase chain reaction (PCR). The result revealed that several genes have shown not only to be regulated by fertility merit but also by the day of oocyte recovery during the estrous cycle. The STAT3 gene was found to be upregulated in oocytes recovered from animals with high fertility merit at both day 0 and day 12. Some other genes like PTTG1, ODC1 and TUBA1C were downregulated at day 0 and upregulated at day 12 in high, compared with low, fertility merit recovered oocytes. In contrast, the transcript abundance of TPM3 was upregulated at day 0 and downregulated at day 12 in high, compared with low, fertility merit recovered oocytes. In addition, ODC1 and STAT3 were found to be associated (P < 0.05) with sperm quality traits as well as flow cytometry parameters. Therefore, the expression of several candidate genes including ODC1 and STAT3 was related to the genetic merit of the cow. In addition polymorphisms in these two genes were found to be associated with bull semen quality.

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“…In cattle [20,21], sheep [41], buffalo [42], and dromedary [43], BCB+ oocytes have yielded better results in terms of blastocysts produced after IVF compared to morphologically selected oocytes. Our findings confirmed the benefit of BCB classification of oocytes in addition to morphological appearance.…”

Section: Discussionmentioning

confidence: 99%

Selection of Immature Cat Oocytes with Brilliant Cresyl Blue Stain Improves In Vitro Embryo Production during Non-Breeding Season

Piras

Ariu

Zedda

et al. 2020

Animals

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In domestic cats, the maturation, fertilization, and development potential in vitro decreases during the non-breeding season. This study aims at evaluating the efficacy of Brilliant Cresyl Blue (BCB) staining in selecting developmentally competent oocytes to be used in in vitro embryo production (IVEP) programs in order to overcome the season variability in blastocyst yield. Cumulus-oocytes complexes (COCs) collected from antral follicles of domestic cat ovaries during the anestrus phase (July to November) were selected by BCB staining and classified as BCB+ (colored cytoplasm) and BCB− (colorless cytoplasm). COCs not exposed to BCB staining were used as control. Before and after in vitro maturation mitochondrial activity and reactive oxygen species (ROS) were measured. Following in vitro fertilization, blastocyst rate, hatching rate, and blastocyst cell numbers were recorded. The results show that BCB staining did not alter the mitochondrial function and ROS production in cat oocytes. BCB+ oocytes presented a higher (p < 0.05) blastocyst rate, hatching rate, and blastocyst cell number than BCB− and control oocytes. In conclusion, BCB staining does not affect the bioenergetic/oxidative status of the oocyte while being a useful tool for selecting good quality oocytes to increase IVEP in domestic cats during non-breeding season.

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Developmental competence of Dromedary camel (Camelus dromedarius) oocytes selected using brilliant cresyl blue staining

Cited by 12 publications

References 38 publications

In vitro embryos production of oocytes selected based on follicular size and by brilliant cresyl blue staining

In vitro embryos production of oocytes selected based on follicular size and by brilliant cresyl blue staining

Transcriptome profile and association study revealedSTAT3gene as a potential quality marker of bovine gametes

Selection of Immature Cat Oocytes with Brilliant Cresyl Blue Stain Improves In Vitro Embryo Production during Non-Breeding Season

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