Developmental expression of a DNA repair gene in Arabidopsis

Shi, Liang; Kent, Robin; Bence, Neil; Britt, Anne B.

doi:10.1016/s0921-8777(97)00023-2

Cited by 32 publications

(27 citation statements)

References 40 publications

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“…These responses vary with the quality and quantity of the damage induced, with the phase of the cell cycle (Jazayeri et al, 2006), and with cell type (Shi et al, 1997). Damage response can also be influenced by environmental inputs (Shor et al, 2013), the age of the organism (Goukassian et al, 2000; Gredilla et al, 2012; Garm et al, 2013), and even the time of day (Ramsey and Ellisen, 2011; Gaddameedhi et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

High atomic weight, high-energy radiation (HZE) induces transcriptional responses shared with conventional stresses in addition to a core â€œDSBâ€ response specific to clastogenic treatments

et al. 2014

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Plants exhibit a robust transcriptional response to gamma radiation which includes the induction of transcripts required for homologous recombination and the suppression of transcripts that promote cell cycle progression. Various DNA damaging agents induce different spectra of DNA damage as well as “collateral” damage to other cellular components and therefore are not expected to provoke identical responses by the cell. Here we study the effects of two different types of ionizing radiation (IR) treatment, HZE (1 GeV Fe26+ high mass, high charge, and high energy relativistic particles) and gamma photons, on the transcriptome of Arabidopsis thaliana seedlings. Both types of IR induce small clusters of radicals that can result in the formation of double strand breaks (DSBs), but HZE also produces linear arrays of extremely clustered damage. We performed these experiments across a range of time points (1.5–24 h after irradiation) in both wild-type plants and in mutants defective in the DSB-sensing protein kinase ATM. The two types of IR exhibit a shared double strand break-repair-related damage response, although they differ slightly in the timing, degree, and ATM-dependence of the response. The ATM-dependent, DNA metabolism-related transcripts of the “DSB response” were also induced by other DNA damaging agents, but were not induced by conventional stresses. Both Gamma and HZE irradiation induced, at 24 h post-irradiation, ATM-dependent transcripts associated with a variety of conventional stresses; these were overrepresented for pathogen response, rather than DNA metabolism. In contrast, only HZE-irradiated plants, at 1.5 h after irradiation, exhibited an additional and very extensive transcriptional response, shared with plants experiencing “extended night.” This response was not apparent in gamma-irradiated plants.

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Section: Introductionmentioning

confidence: 99%

High atomic weight, high-energy radiation (HZE) induces transcriptional responses shared with conventional stresses in addition to a core â€œDSBâ€ response specific to clastogenic treatments

et al. 2014

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“…This pattern of expression is consistent with a requirement for expression in rapidly dividing tissues. Furthermore, high levels of MAG has also been observed in growing leaves, which undergo a relatively low rate of cell division, suggesting that MAG is required not only for DNA replication, but also for cell growth (Shi et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

Base excision repair in sugarcane

et al. 2001

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DNA damage can be induced by a large number of physical and chemical agents from the environment as well as compounds produced by cellular metabolism. This type of damage can interfere with cellular processes such as replication and transcription, resulting in cell death and/or mutations. The low frequency of mutagenesis in cells is due to the presence of enzymatic pathways which repair damaged DNA. Several DNA repair genes (mainly from bacteria, yeasts and mammals) have been cloned and their products characterized. The high conservation, especially in eukaryotes, of the majority of genes related to DNA repair argues for their importance in the maintenance of life on earth. In plants, our understanding of DNA repair pathways is still very poor, the first plant repair genes having only been cloned in 1997 and the mechanisms of their products have not yet been characterized. The objective of our data mining work was to identify genes related to the base excision repair (BER) pathway, which are present in the database of the Sugarcane Expressed Sequence Tag (SUCEST) Project. This search was performed by tblastn program. We identified sugarcane clusters homologous to the majority of BER proteins used in the analysis and a high degree of conservation was observed. The best results were obtained with BER proteins from Arabidopsis thaliana. For some sugarcane BER genes, the presence of more than one form of mRNA is possible, as shown by the occurrence of more than one homologous EST cluster.

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“…Thus, during their dormancy and quiescence periods, there is little opportunity for DNA repair, in accordance with the low expression of BER genes (Shi et al, 1997). However, before germination, plant embryos undergo a period of intense DNA repair prior to the initiation of replication, as a guarantee for the successful growth of seedlings (Osborne, 1993).…”

Section: Base Excision Repair (Ber)mentioning

confidence: 99%

“…The knowledge of the regulation of DNA repair gene expression is essential to the understanding of the biological relevance of this process regarding the resistance to cytotoxic and mutagenic effects of environmental and endogenous DNA-damaging agents (Shi et al, 1997). The expression patterns of specific repair genes in plants have been analyzed by several authors (Shi et al, 1997;Desprez et al, 1998;Liu et al, 2000;Deveaux et al, 2000); however, further studies are required to disclose how such genes and proteins play their roles in different plant tissues.…”

Section: Introductionmentioning

confidence: 99%

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Distribution of DNA repair-related ESTs in sugarcane

et al. 2001

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DNA repair pathways are necessary to maintain the proper genomic stability and ensure the survival of the organism, protecting it against the damaging effects of endogenous and exogenous agents. In this work, we made an analysis of the expression patterns of DNA repair-related genes in sugarcane, by determining the EST (expressed sequence tags) distribution in the different cDNA libraries of the SUCEST transcriptome project. Three different pathways -photoreactivation, base excision repair and nucleotide excision repair -were investigated by employing known DNA repair proteins as probes to identify homologous ESTs in sugarcane, by means of computer similarity search. The results showed that DNA repair genes may have differential expressions in tissues, depending on the pathway studied. These in silico data provide important clues on the potential variation of gene expression, to be confirmed by direct biochemical analysis.

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Developmental expression of a DNA repair gene in Arabidopsis

Cited by 32 publications

References 40 publications

High atomic weight, high-energy radiation (HZE) induces transcriptional responses shared with conventional stresses in addition to a core â€œDSBâ€ response specific to clastogenic treatments

High atomic weight, high-energy radiation (HZE) induces transcriptional responses shared with conventional stresses in addition to a core â€œDSBâ€ response specific to clastogenic treatments

Base excision repair in sugarcane

Distribution of DNA repair-related ESTs in sugarcane

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Developmental expression of a DNA repair gene in Arabidopsis

Cited by 32 publications

References 40 publications

High atomic weight, high-energy radiation (HZE) induces transcriptional responses shared with conventional stresses in addition to a core â€œDSBâ€ response specific to clastogenic treatments

High atomic weight, high-energy radiation (HZE) induces transcriptional responses shared with conventional stresses in addition to a core â€œDSBâ€ response specific to clastogenic treatments

Base excision repair in sugarcane

Distribution of DNA repair-related ESTs in sugarcane

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High atomic weight, high-energy radiation (HZE) induces transcriptional responses shared with conventional stresses in addition to a core â€œDSBâ€ response specific to clastogenic treatments

High atomic weight, high-energy radiation (HZE) induces transcriptional responses shared with conventional stresses in addition to a core â€œDSBâ€ response specific to clastogenic treatments