Developmental expression of immobilin in the rat epididymis

Hermo, Louis; Barin, K.; Oko, Richard

doi:10.1002/ar.1092400109

Cited by 9 publications

(9 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The possibility that circulating androgens regulate the expression of SGP-2 in the distal initial segment and caput can probably be excluded because Cyr and Robaire (1992) demonstrated in adult rats that neither orchidectomy nor testosterone replacement had any effect on SGP-2 mRNA concentrations in these regions. Although a similar shift in expression to the proximal part of the epididymis is observed for SGP-2 and immobilin (Hermo et al, 1994) early in postnatal development, our ligation results indicate that the factors regulating the production of these two proteins at least in the caput epididymidis differ. Ligation represses immobilin expression in the caput epididymidis (Hermo et al, 1994) but has no effect on SGP-2 expression.…”

Section: Discussionsupporting

confidence: 56%

“…Although a similar shift in expression to the proximal part of the epididymis is observed for SGP-2 and immobilin (Hermo et al, 1994) early in postnatal development, our ligation results indicate that the factors regulating the production of these two proteins at least in the caput epididymidis differ. Ligation represses immobilin expression in the caput epididymidis (Hermo et al, 1994) but has no effect on SGP-2 expression. Cyr and Robaire (1992) showed that orchidectomy in adult rats resulted in a substantive increase in SGP-2 mRNA concentrations in the corpus and cauda epididymidis and that testosterone replacement caused a dose dependent decrease in SGP-2 mRNA levels.…”

Section: Discussionsupporting

confidence: 56%

“…The regional shift of SGP-2 synthesis and secretion to the proximal region of the epididymis that occurs relatively early in postnatal development is reminiscent of the results obtained with immobilin (Hermo et al, 1994). Also similar is the developmental loss in SGP-2 synthesis in the proximal initial segment by day 39.…”

Section: Discussionmentioning

confidence: 63%

“…Also different is the moderate to intense expression of SGP-2 by principal cells in the distal cauda by day 49 and in the corpus and proximal cauda by day 56. Immobilin is never expressed in any of these regions after day 28 (Hermo et al, 1994).…”

Section: Discussionmentioning

confidence: 95%

“…This difference suggests that luminal factors originating from the testis are normally inhibitory for SGP-2 expression in this region and may accumulate by day 39 to have this effect. Although the nature of these inhibitory factors are unknown it is possible that they have an inhibitory effect on other secretory proteins of this region as well because under a similar experimental protocol immobilin, which is also normally repressed in this region by day 39, continues to be expressed a t high levels in efferent duct ligated rats (Hermo et al, 1994). Second, the moderate to intense levels of SGP-2 that normally appear in the corpus and proximal cauda regions of the epididymis on day 56 are repressed in ligated rats.…”

Section: Discussionmentioning

confidence: 99%

See 4 more Smart Citations

Developmental expression of sulfated glycoprotein‐2 in the epididymis of the rat

1994

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 56%

Section: Discussionsupporting

confidence: 56%

Section: Discussionmentioning

confidence: 63%

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Developmental expression of sulfated glycoprotein‐2 in the epididymis of the rat

1994

Self Cite

View full text Add to dashboard Cite

show abstract

Expression and regulation of LRP-2/megalin in epithelial cells lining the efferent ducts and epididymis during postnatal development

et al. 1999

View full text Add to dashboard Cite

Low density lipoprotein receptor‐related protein‐2/megalin (LRP‐2) is a receptor belonging to the low density lipoprotein receptor family that mediates endocytosis and lysosomal degradation of a variety of ligands including apolipoprotein J (Apo J)/clusterin/SGP‐2. LRP‐2 has been shown to be expressed regionally in the adult rat epididymis. In this study, we describe the pattern of expression of LRP‐2 in the efferent ducts and epididymis during postnatal development of the rat and examine the role of testicular luminally derived substances on its expression. The expression of LRP‐2 was analyzed immunocytochemically in tissues of normal animals ranging in age from postnatal day 7–90 and in 15‐day‐old efferent‐duct‐ligated animals sacrificed at later ages. In the efferent ducts, LRP‐2 expression, appearing as a dense band on the apical surface of the nonciliated epithelial cells, was noted as early as day 7, well before the entry of sperm, Sertoli‐cell‐derived secretory products, and high levels of androgens. Efferent duct ligation studies further revealed that expression under this condition was comparable to controls at all later ages examined, suggesting that the factor regulating its expression was not a luminally derived testicular substance. In normal untreated animals, LRP‐2 expression was not apparent at any of the ages examined in the proximal initial segment of the epididymis. By comparison, the distal initial segment, although having no LRP‐2 expression from 7–15 days, showed expression in principal cells by day 21 which intensified at days 29 and 39. However, by day 49 and at later ages (56 and 90), LRP‐2 immunoreactivity over principal cells became spotty or with weak or moderate reactivity in some cells and none in others. LRP‐2 expression in the intermediate zone, proximal caput, corpus, and cauda regions also appeared in principal cells by day 21, intensified at days 29 and 39 and persisted as such at all later ages examined, correlating with high levels of androgens shown to occur by day 39. Although LRP‐2 expression in the distal caput region was evident in principal cells at days 21 and 29, it became spotty with weak, moderate, or absent reactivity over principal cells at all later ages. These data suggest that LRP‐2 expression is under the influence of both stimulatory and region‐specific inhibitory factors. Analysis of 15‐day‐old efferent‐duct‐ligated animals at all later ages examined revealed that there was no change in LRP‐2 expression along the entire epididymis, suggesting that both the stimulatory and inhibitory factors are not luminally derived testicular substances. The observed pattern of LRP‐2 expression in all regions of the epididymis, except the distal caput region, was similar to that described for Apo J internalization by principal cells during postnatal development, showing a correlation between LRP‐2 expression and its ligand, Apo J. In summary, LRP‐2 expression in the epididymis undergoes region‐specific changes during postnatal development and appears to be influence...

show abstract

Developmental expression of the glutathione S‐transferase Yo subunit in the rat testis and epididymis using light microscope immunocytochemistry

1994

View full text Add to dashboard Cite

show abstract

Developmental expression of immobilin in the rat epididymis

Cited by 9 publications

References 27 publications

Developmental expression of sulfated glycoprotein‐2 in the epididymis of the rat

Developmental expression of sulfated glycoprotein‐2 in the epididymis of the rat

Expression and regulation of LRP-2/megalin in epithelial cells lining the efferent ducts and epididymis during postnatal development

Developmental expression of the glutathione S‐transferase Yo subunit in the rat testis and epididymis using light microscope immunocytochemistry

Contact Info

Product

Resources

About