Devosia geojensis sp. nov., isolated from diesel-contaminated soil in Korea

Abstract

A Gram-negative, strictly aerobic, non-spore-forming bacterium, designated strain BD-c194 T , was isolated from diesel-contaminated soil in Geoje, Korea. The cells were short, motile rods with single polar flagella. Growth of strain BD-c194 T was observed between 15 and 45 6C (optimally at 30-35 6C) and between pH 6.0 and 9.5 (optimally at pH 7.5-9.0). The predominant fatty acids were 11-methyl C 18 : 1 v7c, C 16 : 0 , C 18 : 1 v7c, C 18 : 0 and an unknown fatty acid (equivalent chain-length 18.814); a large amount of phosphatidylglycerol and a small amount of diphosphatidylglycerol were present as polar lipids. The G+C content of the genomic DNA was 60.8 mol% and the major isoprenoid quinone was Q-10. A comparative 16S rRNA gene sequence analysis showed that strain BD-c194 T formed a well-defined phyletic lineage within the genus Devosia (with 100 % bootstrap support). The levels of 16S rRNA gene sequence similarity with respect to the type strains of other Devosia species ranged from 95.0 to 96.1 %. On the basis of chemotaxonomic data and molecular properties, strain BD-c194 T represents a novel species within the genus Devosia, for which the name Devosia geojensis sp. nov. is proposed. The type strain is BD-c194 T (5KCTC 22082 T 5DSM 19414 T). The genus Devosia (family Hyphomicrobiaceae, order Rhizobiales, class Alphaproteobacteria) was first described by Nakagawa et al. (1996) with the transfer of 'Pseudomonas riboflavina' (Foster, 1944) to Devosia riboflavina. At the time of writing, the genus comprises six species: Devosia riboflavina (Nakagawa et al., 1996), D. neptuniae (Rivas et al., 2003), D. limi (Vanparys et al., 2005), D. soli (Yoo et al., 2006), D. insulae (Yoon et al., 2007) and D. subaequoris (Lee, 2007). In the course of the screening of microorganisms from contaminated soil, we isolated a novel bacterium, designated strain BD-c194 T , belonging to the genus Devosia. Here we describe the polyphasic analysis used to determine its taxonomic position. Diesel-contaminated soil was collected from the diesel-contaminated soil surface of a gas (petrol) station on Geoje Island, Korea. The total petroleum hydrocarbon content of the contaminated soil was about 2000 mg kg 21. The soil sample was serially diluted with a 1 % (w/v) saline solution and spread on R2A agar (Difco). After 5 days incubation at 25 u C, strain BD-c194 T was selected from an agar plate and analysed. Although strain BD-c194 T was isolated from diesel-contaminated soil, it did not grow on minimal agar medium (Stanier et al., 1966) containing diesel as the sole carbon and energy source, showing that the strain is not a diesel degrader. The morphological, physiological and biochemical characteristics of strain BD-c194 T were examined using routine cultivation on R2A medium at 30 u C for 3 days (except where indicated otherwise). The type strains of some related taxa (see Table 1) were used as reference strains for the biochemical tests. The temperatures and pH for growth of strain BD-c194 T were investigated by growing the isolate on R2A...

Devosia honganensis sp. nov., isolated from the soil of a chemical factory

“…Minor amounts of unidentified glycolipids and unidentified polar lipids were also detected (Fig. The major fatty acids, polar lipids and DNA G?C content are in accordance with those of members of the genus Devosia (Nakagawa et al 1996;Rivas et al 2003;Vanparys et al 2005;Ryu et al 2008). The presence of phosphatidylglycerol as the major polar lipid is consistent with the lipid profile of D. geojensis BD-c194 T (Ryu et al, 2008).…”

“…Gram staining reaction was performed following the method as described by Beveridge et al (2007). All physiological and biochemical tests on strain NSL10 T were performed simultaneously with the closely related reference strains Devosia albogilva IPL15 T (Verma et al 2009) and Devosia geojensis BD-c194 T (Ryu et al 2008). Growth under anaerobic conditions was determined on LB medium using the GasPak Anaerobic Systems (BBL) according to the manufacturer's instructions.…”

Devosia nitraria sp. nov., a novel species isolated from the roots of Nitraria sibirica in China

“…The major fatty acid is C 16:0 (36.8%) with minor amounts of C 16:0 N alcohol (14.6%), 10-methyl C 17:0 (12.6%), C 18:1 ω 7 c (9.4%), C 18:0 (7.3%), C 19:0 cyclo ω8 c (5.5%), 11-Methyl C 18:1 ω7c (4.7%) and C 18:0 3-OH (2.5%) (Table 3). The cellular fatty acid profile was similar to the reference strains D. geojensis BD-c194 T (Ryu et al 2008) and D. pacifica NH131 T (Jia et al 2014) that have C 16:0 and C 18:1 ω 7 c as their main lipids, respectively. A large amount of unidentified glycolipid, diphosphatidylglycerol, phosphatidylglycerol and a small amount of unidentified polar lipids were present as polar lipids for strain 36-5-1 T (Fig.…”

Maritalea myrionectae gen. nov., sp. nov., isolated from a culture of the marine ciliate Myrionecta rubra

“…Selected characteristics that differentiate strain CL-SK30 T (Maritalea myrionectae gen. nov., sp. nov.) from related genera in the family Hyphomicrobiaceae Taxa: 1, strain CL-SK30 T (data from this study); 2, C. marinus CL-GR60 T (Hwang & Cho, 2008); 3, Devosia (for type strains: Nakagawa et al, 1996;Rivas et al, 2003;Yoo et al, 2006;Lee, 2007;Yoon et al, 2007;Ryu et al, 2008). +, Positive; 2, negative; V, variable; Q, ubiquinone.…”