Dextran sieving test for characterization of virus filtration membranes

Bakhshayeshi, Meisam; Kanani, Dharmesh M.; Mehta, Amit; Reis, Robert van; Kuriyel, Ralf; Jackson, Nigel B.; Zydney, Andrew L.

doi:10.1016/j.memsci.2011.05.067

Cited by 26 publications

(12 citation statements)

References 21 publications

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“…The slightly asymmetric structure of the Ultipor VF Grade DV20 membrane, with a more open pore size in the reservoir zone and a tighter pore size near the filter exit, has been observed previously. In contrast to scanning electron microscopy cross‐sections showing relative homogeneity, dextran retention tests performed with the membrane oriented with the shiny‐side up yielded contrasting results to tests with the shiny‐side down . Further demonstration of this asymmetry was provided by filtration of the 20 nm gold colloid nanoparticles .…”

Section: Results and Analysismentioning

confidence: 89%

“…In contrast to scanning electron microscopy cross-sections showing relative homogeneity, dextran retention tests performed with the membrane oriented with the shiny-side up yielded contrasting results to tests with the shiny-side down. 20 Further demonstration of this asymmetry was provided by filtration of the 20 nm gold colloid nanoparticles. 18 When the DV20 membrane was challenged in the normal orientation (shiny-side down), all of the gold particles were retained within the membrane, with no measureable increase in the concentration of gold particles in the bulk solution in the stirred cell.…”

Section: Internal Polarization Modelmentioning

confidence: 99%

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Internal virus polarization model for virus retention by the Ultipor^®VF Grade DV20 membrane

Jackson

Bakhshayeshi

Zydney

et al. 2014

Biotechnology Progress

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Several recent studies have reported a decline in virus retention during virus challenge filtration experiments, although the mechanism(s) governing this phenomenon for different filters remains uncertain. Experiments were performed to evaluate the retention of PP7 and PR772 bacteriophage through Ultipor VF Grade DV20 virus filters during constant pressure filtration. While the larger PR772 phage was fully retained under all conditions, a 2-log decline in retention of the small PP7 phage was observed at high throughputs, even under conditions where there was no decline in filtrate flux. In addition, prefouling the membrane with an immunoglobulin G solution had no effect on phage retention. An internal polarization model was developed to describe the decline in phage retention arising from the accumulation of phage in the upper (reservoir) layer within the filter which increases the challenge to the lower (rejection) layer. Independent support for this internal polarization phenomenon was provided by confocal microscopy of fluorescently labeled phage within the membrane. The model was in good agreement with phage retention data over a wide range of phage titers, confirming that virus retention is throughput dependent and supporting current recommendations for virus retention validation studies. These results provide important insights into the factors governing virus retention by membrane filters and their dependence on the underlying structure of the virus filter membrane.

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Section: Results and Analysismentioning

confidence: 89%

Section: Internal Polarization Modelmentioning

confidence: 99%

Internal virus polarization model for virus retention by the Ultipor^®VF Grade DV20 membrane

Jackson

Bakhshayeshi

Zydney

et al. 2014

Biotechnology Progress

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“…In the internal polarization model, the phage that enter the membrane are assumed to either be immediately captured within a highly constricted pore or they begin to accumulate in a “reservoir zone” in the upper region of the membrane. The phage are retained in this upper reservoir zone by the tighter (smaller diameter) pores as one moves deeper within the DV20 (Bakhshayeshi et al, ). The increase in phage transmission during the filtration arises from the increase in phage concentration within the reservoir zone, causing the retentive layer to be continuously challenged by a higher concentration of phage.…”

Section: Results and Analysismentioning

confidence: 99%

Effects of a pressure release on virus retention with the Ultipor DV20 membrane

Woods

Zydney

2013

Biotech & Bioengineering

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Recent data have shown that a temporary release in the transmembrane pressure can cause a significant increase in virus transmission through a number of commercial virus filters. The objective of this work was to study the effect of a pressure release on retention of the bacteriophage ΦX174 by the Ultipor DV20 membrane, with phage capture within the membrane visualized by confocal microscopy. Phage challenge tests showed a significant transient increase in phage transmission (typically by an order of magnitude) immediately after a pressure release. This transient increase was not due to any damage to the filter or to the presence of a back-flow (from permeate to feed). Confocal images demonstrated that the pressure release caused the migration of previously captured bacteriophage further into the depth of the filter. Data were analyzed using a modified internal polarization model, with the results providing important insights into the factors controlling virus retention during virus filtration.

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“…As soluções de dextranas são muito utilizadas para determinar índices de retenção e distribuição do tamanho de poros, pois não interagem com a maioria das membranas, possuem pesos moleculares conhecidos e são de baixo custo (Bakhshayeshi et al, 2011). Foram medidos os fluxos em diferentes pressões para cada solução de dextrana e poli(vinilpirrolidona), a Figura 4 ilustra os valores obtidos e sua representação gráfica.…”

Section: Ensaios De Retençãounclassified

Caracterização Morfológica E Parâmetros Funcionais De Membrana Cerâmica Multitubular

2017

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Dextran sieving test for characterization of virus filtration membranes

Cited by 26 publications

References 21 publications

Internal virus polarization model for virus retention by the Ultipor^®VF Grade DV20 membrane

Internal virus polarization model for virus retention by the Ultipor^®VF Grade DV20 membrane

Effects of a pressure release on virus retention with the Ultipor DV20 membrane

Caracterização Morfológica E Parâmetros Funcionais De Membrana Cerâmica Multitubular

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Dextran sieving test for characterization of virus filtration membranes

Cited by 26 publications

References 21 publications

Internal virus polarization model for virus retention by the Ultipor®VF Grade DV20 membrane

Internal virus polarization model for virus retention by the Ultipor®VF Grade DV20 membrane

Effects of a pressure release on virus retention with the Ultipor DV20 membrane

Caracterização Morfológica E Parâmetros Funcionais De Membrana Cerâmica Multitubular

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Internal virus polarization model for virus retention by the Ultipor^®VF Grade DV20 membrane

Internal virus polarization model for virus retention by the Ultipor^®VF Grade DV20 membrane