Dextrin characterization by high-performance anion-exchange chromatography–pulsed amperometric detection and size-exclusion chromatography–multi-angle light scattering–refractive index detection

Quantification of sugars in wheat flours with an HPAEC-PAD method

“…The most common mobile phases found in the literature for analysis of sugars by HPAEC-PAD have been NaOH/Water (Martínez et al, 2005), NaOH/NaAcO/Water (Allosio-Ouarnier et al, 2000;Ammeraal et al, 1991;Weston & Brocklebank, 1999) or NaOH/NaAcO (Ba et al, 2013;Gelders et al, 2003;Koch, Andersson, & Åman, 1998;Kong, Bertoft, Bao, & Corke, 2008;White et al, 2003) in gradient mode. Since mobile phases with a pH higher than 12 (which allows the deprotonation of studied sugars) are needed, sodium hydroxide have been always employed.…”

“…Most publications that analyse these sugars in different cereal matrices with HPAEC-PAD do not study isomaltose (Ammeraal, Delgado, Tenbarge, & Friedman, 1991;Gelders et al, 2003;White, Hudson, & Adamson, 2003), although this is important in human health due to congenital sucrase-isomaltase deficiency (Sander et al, 2005). Isomaltose is naturally present in manuka honey (Weston & Brocklebank, 1999) but also in starch hydrolysis (Ba et al, 2013).…”

Simultaneous Separation and Quantification of Linear Xylo- and Cello-Oligosaccharides Mixtures in Lignocellulosics Processing Products on High-Performance Anion-Exchange Chromatography Coupled with Pulsed Amperometric Detection

“…Various chromatographic devices combined with a mass spectrometry (MS) detector have been suggested. However, TLC and HPLC are suitable for mono-, bi-, and a few trisaccharides rather than for higher oligo-or polysaccharides (Gauch et al 1979;Raessler 2011), whereas HPAEC-PAD has been applied just for gluco-or fruco-oligosaccharides from starch, alginate, and inulin ( Van der Hoeven et al 1992;White et al 2003;Balance et al 2005;Corradini et al 2012). Despite the apparent priority of molecular structural analysis and qualitative identification of compounds, chromatography combined with MS detection has been somewhat inconvenient in terms of a permanent online application due to the extended experimental setup.…”

Ultrasonic wave spectroscopy study of sugar oligomers and polysaccharides in aqueous solutions: The hydration length concept

“…The result of the partial degradation is characterised by a wide molecular weight distribution ranging from the monomer (glucose) to degrees of polymerisation (DP) higher than 1000 as shown using size exclusion chromatography [1].…”