Simultaneous Separation and Quantification of Linear Xylo- and Cello-Oligosaccharides Mixtures in Lignocellulosics Processing Products on High-Performance Anion-Exchange Chromatography Coupled with Pulsed Amperometric Detection

Xu, Yong; Fan, Li; Wang, Xing; Yong, Qiang; Yu, Shiyuan

doi:10.15376/biores.8.3.3247-3259

Cited by 47 publications

(19 citation statements)

References 29 publications

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“…The reaction mixture contained 0.1 M acetate buffer (pH 6.0) with 10 µL enzyme and 1 mgmL −1 of 1,4-β-linked CMC and oligosaccharides (CMC, cellopentaose, cellotetraose, or cellotriose). The reaction was carried out at 35 • C, and the samples were collected after 24 h and further characterized by HPAEC-PAD as previously described by Xu et al (2013).…”

Section: Hpaec-pad Analysismentioning

confidence: 99%

A Novel Cold-Adaptive Endo-1,4-β-Glucanase From Burkholderia pyrrocinia JK-SH007: Gene Expression and Characterization of the Enzyme and Mode of Action

Chen

Kameshwar

et al. 2020

Front. Microbiol.

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The efficient industrial conversion of plant-derived cellulose to simple sugars and other value-added chemicals requires various highly stable and reactive enzymes. Industrial processes especially synchronous saccharification and fermentation (SSF)based production of cellulosic bio-ethanol require enzymes that are active at lower temperatures. In this study, we have identified, characterized, and expressed the cold-adaptive endo-1,4-β-glucanase (BpEG) isolated from the Burkholderia pyrrocinia JK-SH007. The analysis of the predicted amino acid sequence indicated that BpEG belongs to GH family 8. The BpEG without the signal peptide was cloned into the expression vector pET32a and significantly expressed in Escherichia coli BL21 (DE3) competent cells. The SDS-PAGE and Western blot analysis of BpEG revealed that the recombinant BpEG was approximately 60 kDa. Purified recombinant BpEG exhibited hydrolytic activity against carboxymethyl cellulose (CMC) and phosphoric acid swollen cellulose (PASC), but not crystalline cellulose and xylan substrates. High performance, anion exchange, chromatography-pulsed amperometric detector (HPAEC-PAD) analysis of the enzymatic products obtained from depolymerization of 1,4-β-linked biopolymers of different lengths revealed an interesting cutting mechanism employed by endoglucanases. The recombinant BpEG exhibited 6.0 of optimum pH and 35 • C of optimum temperature, when cultured with CMC substrate. The BpEG enzyme exhibited stable activity between pH 5.0 and 9.0 at 35 • C. Interestingly, BpEG retained about 42% of its enzymatic activity at 10 • C compared to its optimal temperature. This new cold-adaptive cellulase could potentially achieve synchronous saccharification and fermentation (SSF) making BpEG a promising candidate in the fields of biofuel, biorefining, food and pharmaceutical industries.

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Section: Hpaec-pad Analysismentioning

confidence: 99%

A Novel Cold-Adaptive Endo-1,4-β-Glucanase From Burkholderia pyrrocinia JK-SH007: Gene Expression and Characterization of the Enzyme and Mode of Action

Chen

Kameshwar

et al. 2020

Front. Microbiol.

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“…Carbon sources for bacteria included the oligosaccharides to be tested: purified HMOS from pooled human milk [29], GMOS produced from Konjac powder, GOS from transgalactosylation of lactose (Clasado, UK), XOS from corncob [30], CBS, glucose, xylose, and mannose from SigmaAldrich Chemical Co. (St. Louis, MO) and 2′-FL from Glycosyn, LLC. (Waltham, MA, USA).…”

Section: Fermentation Substratesmentioning

confidence: 99%

Relative fermentation of oligosaccharides from human milk and plants by gut microbes

Wang

Chen

et al. 2016

Eur Food Res Technol

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sparse. B. longum fermentation products inhibited C. jejuni and E. coli. Thus, HMOS most strongly promoted growth of the two mutualists, and both HMOS and GMOS were efficiently fermented by these mutualists into organic acids. This is consistent with a primary role of HMOS in guiding early colonization of the infant microbiota by mutualist symbionts, and of plant oligosaccharides, especially GMOS, in maintaining a favorable microbiota through adulthood.Keywords Human milk oligosaccharides · Plant oligosaccharides · Microbiota · Prebiotic · Organic acids Abbreviations HMOSHuman milk oligosaccharides GMOS Gluco-manno-oligosaccha-Abstract Gut microbiota is important to human health. Specific dietary glycans promote favorable microbiota growth and inhibit pathobionts. Dietary glycans most relevant to adults and weaned infants are derived from plants or lactose; human milk oligosaccharides (HMOS) are most relevant to breastfed infants. Their efficacy in supporting bacterial growth is compared to determine their potential roles in the initiation and maintenance of colonization. Bioactivities of gluco-manno-oligosaccharides (GMOS), galacto-oligosaccharides (GOS), xylo-oligosaccharides (XOS), cellobiose (CBS), HMOS, and the most prominent individual HMOS, 2′-fucosyllactose (2′-FL) were contrasted. Two representative gut microbiota mutualists, Bifidobacteria longum ATCC15697 and Lactobacillus acidophilus NRRL B-4495, and two non-mutualists, Campylobacter jejuni S107 and Escherichia coli K12, were used to assess the in vitro prebiotic potential of these oligosaccharides. All oligosaccharides afforded growth of B. longum and L. acidophilus, with HMOS supporting the most robust growth, while none of these oligosaccharides afforded meaningful growth of non-mutualists. B. longum efficiently converted HMOS, GMOS, GOS, and XOS into organic acid fermentation products, and, to a lesser degree, L. acidophilus metabolized HMOS, GMOS, and GOS. Fermentation of these glycans by C. jejuni and E. coli was

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“…The external standards included XOS with polymerization degrees of two to six. Two solutions of 100 mmol/L sodium hydroxide (NaOH) and 500 mmol/L sodium acetate (NaAc) solution were used as mobile phase at a flow rate of 0.3 mL/min . Each group of experiments was repeated twice.…”

Section: Methodsmentioning

confidence: 99%

Optimization of selective acidolysis pretreatment for the valorization of wheat straw by a combined chemical and enzymatic process

Guo

Huang

Zhang

et al. 2019

J of Chemical Tech & Biotech

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BACKGROUND: Advances in industrial biotechnology offer potential opportunities for economic utilization of abundantly available agricultural crop waste such as wheat straw, which is of great interest for advancement in xylo-oligosaccharides (XOS) and fermentable monosaccharides. In the present study, crude XOS and fermentable monosaccharides were sequentially produced by a combined chemical and enzymatic process from wheat straw.RESULTS: Maximum yield of XOS was 38.2% and the corresponding yield of glucose was 82.7% despite the wax barrier on the surface of wheat straw, while the acetic acid concentration was decreased from 20% to 5%. Pilot scale run produces 91.6 g XOS, 92.5 g xylose and 316.0 g glucose from 1000 g of wheat straw that means the utilization of 78.9% of xylan and 89.7% glucan constitutes.CONCLUSION: By removal of 65% xylan from wheat straw, enzymatic hydrolysis of cellulose was also improved efficiently although its polymer structure was almost kept inert during acetic acid pretreatment. ABBREVIATIONSAA acetic acid DC degraded chemicals HMF hydroxymethyl furfural SSTs stainless steel tubes XOS xylo-oligosaccharides Analytical method The sugars and degraded chemicals were detected by high-performance liquid chromatography (HPLC, Agilent 1260, J Chem Technol Biotechnol 2020; 95: 694-701Figure 3. (a) Enzymatic hydrolysis at various cellulase loading; (b) main sugar contents variations in 15 FPIU/g enzymatic hydrolysis; (c) changes of polysaccharide components in wheat straw before and after enzymatic hydrolysis and pretreatment. J Chem Technol Biotechnol 2020; 95: 694-701

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Simultaneous Separation and Quantification of Linear Xylo- and Cello-Oligosaccharides Mixtures in Lignocellulosics Processing Products on High-Performance Anion-Exchange Chromatography Coupled with Pulsed Amperometric Detection

Cited by 47 publications

References 29 publications

A Novel Cold-Adaptive Endo-1,4-β-Glucanase From Burkholderia pyrrocinia JK-SH007: Gene Expression and Characterization of the Enzyme and Mode of Action

A Novel Cold-Adaptive Endo-1,4-β-Glucanase From Burkholderia pyrrocinia JK-SH007: Gene Expression and Characterization of the Enzyme and Mode of Action

Relative fermentation of oligosaccharides from human milk and plants by gut microbes

Optimization of selective acidolysis pretreatment for the valorization of wheat straw by a combined chemical and enzymatic process

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