Di-(2-ethylhexyl)phthalate and Pyranon Derivated from Endophytic fungi &lt;i&gt;Penicillium&lt;/i&gt; sp the Leave of Kunyit Putih (&lt;i&gt;Curcuma zedoaria&lt;/i&gt;)

Muharni, Muharni; Fitrya, Fitrya; Ruliza, Milanti Okta; Susanti, Dwi Anjar; Elfita, Elfita

doi:10.22146/ijc.21241

Cited by 10 publications

(5 citation statements)

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“…DEHP is used as plasticizer in the plastics industry and is considered as a persistent environmental pollutant due to its extensive usage [ 241 ]. DEHP was isolated from endophytic microorganisms [ 242 , 243 ], but not from those belonging to the genus Diaporthe . Hence, this is the first report on the production of DEHP by species of the genus Diaporthe .…”

Section: Resultsmentioning

confidence: 99%

Diversity of cultivable fungal endophytes in Paullinia cupana (Mart.) Ducke and bioactivity of their secondary metabolites

et al. 2018

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Paullinia cupana is associated with a diverse community of pathogenic and endophytic microorganisms. We isolated and identified endophytic fungal communities from the roots and seeds of P. cupana genotypes susceptible and tolerant to anthracnose that grow in two sites of the Brazilian Amazonia forest. We assessed the antibacterial, antitumor and genotoxic activity in vitro of compounds isolated from the strains Trichoderma asperellum (1BDA) and Diaporthe phaseolorum (8S). In concert, we identified eight fungal species not previously reported as endophytes; some fungal species capable of inhibiting pathogen growth; and the production of antibiotics and compounds with bacteriostatic activity against Pseudomonas aeruginosa in both susceptible and multiresistant host strains. The plant genotype, geographic location and specially the organ influenced the composition of P. cupana endophytic fungal community. Together, our findings identify important functional roles of endophytic species found within the microbiome of P. cupana. This hypothesis requires experimental validation to propose management of this microbiome with the objective of promoting plant growth and protection.

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Section: Resultsmentioning

confidence: 99%

Diversity of cultivable fungal endophytes in Paullinia cupana (Mart.) Ducke and bioactivity of their secondary metabolites

et al. 2018

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“…Individual hyphal tips were transferred into fresh PDA and incubated at 30°C for seven days. The pure cultures were numbered, maintained in PDA slants, and kept at 4°C (Muharni et al 2014;).…”

Section: Isolation Of Endophytic Fungimentioning

confidence: 99%

Diversity of endophytic fungi in Syzygium aqueum

et al. 2021

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Abstract. Habisukan UH, Elfita, Widjajanti H, Setiawan A, Kurniawati AR. 2021. Diversity of endophytic fungi in Syzygium aqueum. Biodiversitas 22: 1129-1137. Exploring endophytic fungi may provide alternative, plant-based ethnomedicines. The discovery of endophytic fungi can produce many plant-derived drugs that give new horizons to the pharmaceutical industry for the availability and production of such medicines on a large scale. Extracts from Syzygium aqueum Alston (jambu air), a fruit-bearing plant native to Indonesia, have long been used in traditional medicine. Therefore, the purpose of this study was to identify, and investigate, the antimicrobial activity of the endophytic fungi found in S. aqueum. Results revealed that total16 fungi were isolated . Fungal endophytes were identified through morphological characterization and subsequently compared with key fungal identification books. The ethyl acetate extracts of the isolated endophytic fungi were screened for their antimicrobial activity through paper disc diffusion assay fungi isolated from S. aqueum were identified as: Aspergillus niger (Isolate code R21, R41, T72), Cylindrocarpon sp. (Isolate code R11, R31), Trichoderma aureoviridae (Isolate code T11), Trichoderma harzianum (Isolate code T21), Trichoderma sp. (Isolate code T31), Pestalotia sp. (Isolate code T41and T62), Beltrania sp. (Isolate code T52), Chaetomium sp. (Isolate code L11), Cochliobolus sp. (Isolate code L22), Penicillium sp. (Isolate code L32), Cylindrocladium sp. (Isolate code L42), and an unidentified Ascomycota (T51). The values of Shannon-Weiner and Simpson diversity indexes for the overall fungal community were H′=2.133 and 1-D=0.859 respectively. All isolates showed potential antimicrobial activity against Escherichia coli, Salmonella Typhi, and Staphylococcus aureus, but the lowest activity against Candida albicans. The results indicate that the bioactive compounds and secondary metabolites of these isolates should be further investigated for pharmaceutical applications.

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“…The separation process was carried out by moving single type of colony to new PDA medium in a streak plate and incubated at 37 C for 2 x 24 hours. The pure fungi colony then prepared for working culture and stock culture by growing it in skewed PDA medium (Muharni, Fitrya, Ruliza, Susanti, & Elfita, 2014;Barik, Tayung, Jagadev, & Dutta, 2010;Khan et al, 2010).…”

Section: Sterilization Of S Samarangense Leaves and Isolation Of Endmentioning

confidence: 99%

Antioxidant Activity of Syzygium samarangense L. and Their Endophytic Fungi

Budiono

Elfita

Muharni

et al. 2019

Molekul

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Leaves of jambu air (Syzygium samarangense L.) has been used by local residents as medicine for various diseases caused by free radical agents in human’s body. This study aims to find a new source of antioxidants from medicinal plants and their endophytic fungi Syzygium samarangense leaves was fractionated by maceration method using gradient solvent i.e. n-hexane, ethyl acetate, and methanol. The antioxidant activity of the leaf fractions was determined using 1,1diphenyl-2-picryl hydrazyl (DPPH) method. The chemical compound was isolated from active fraction by chromatographic techniques and their chemical structure was identified using spectroscopy techniques. The endophytic fungi was isolated from S. samarangense leaves and continued to cultivation in potato-dextrose broth (PDB) medium for four weeks. The medium was extracted using ethyl acetate and determined its antioxidant activity. Fungi’s isolate with highest activity was analyzed to identify its molecular. Ethyl acetate fraction of S. samarangense leaves showed highest antioxidant activity. Spectroscopy analysis result concluded the isolated compound is 5,7-dihydroxy-6,8-dimethyl flavanone. Four endophytic fungi had been isolated form S. samarangense leaves. Antioxidant activity test showed that ethyl acetate extract of endophytic fungi BJA-1 has the highest value. Molecular identification of BJA-1 shows high homology with Lasiodiplodia venezuelensis strain CBS 129753.

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Di-(2-ethylhexyl)phthalate and Pyranon Derivated from Endophytic fungi <i>Penicillium</i> sp the Leave of Kunyit Putih (<i>Curcuma zedoaria</i>)

Cited by 10 publications

References 0 publications

Diversity of cultivable fungal endophytes in Paullinia cupana (Mart.) Ducke and bioactivity of their secondary metabolites

Diversity of cultivable fungal endophytes in Paullinia cupana (Mart.) Ducke and bioactivity of their secondary metabolites

Diversity of endophytic fungi in Syzygium aqueum

Antioxidant Activity of Syzygium samarangense L. and Their Endophytic Fungi

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