Diagnosis of dengue infection by detecting specific immunoglobulin M antibodies in saliva samples

Oliveira, Solange Artimos de; Rodrigues, C. R.; Camacho, L. A. B.; Miagostovich, M P; Araújo, ElianeS.M; Nogueira, R. M.

doi:10.1016/s0166-0934(98)00139-6

Cited by 21 publications

(13 citation statements)

References 17 publications

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“…The sensitivity of IgG detection was much higher (91.9%) than IgM, but again with a large difference between the study sites. Our findings fall within the range of sensitivity and specificity estimates published previously for detection of DENV-specific IgM and IgG from oral fluid samples,7–10,20 including both those that used a swab device and those where saliva was collected directly. We did not investigate DENV-specific IgA, but others have reported a high sensitivity and specificity for diagnosis of acute dengue using serum or DBS for IgA detection (though poor results using saliva)10 and a dengue IgA rapid test is commercially available (ASSURE; MP Biomedicals, Singapore).…”

Section: Discussionsupporting

confidence: 84%

An Evaluation of Dried Blood Spots and Oral Swabs as Alternative Specimens for the Diagnosis of Dengue and Screening for Past Dengue Virus Exposure

Anders¹,

Nguyet²,

Quyen³

et al. 2012

The American Society of Tropical Medicine and Hygiene

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Non-invasive specimens for dengue diagnosis may be preferable where venous blood is difficult to collect and/or process, such as community-based or remote settings or when sampling from young children. We evaluated the performance of oral swabs and dried blood spots (DBS), compared with plasma, in diagnosing acute dengue and screening for past dengue virus (DENV) exposure. DENV-specific immunoglobulin (Ig) M, IgG, and NS1 antigen were detected both in oral swabs and DBS from acute patients. Oral swabs were less sensitive (IgM: 68.7%, IgG: 91.9%, NS1: 64.7%), but retained good specificity (100%, 92.3%, 95.8%, respectively) compared with plasma. DBS displayed high sensitivity (IgM: 100%, IgG: 96%, NS1: 100%) and specificity (IgM: 75%, IgG: 93%). DENV RNA was amplified from DBS (sensitivity 95.6%) but not from oral swabs. DENV-IgG (indicative of past flavivirus exposure) were detected with moderate sensitivity (61.1%) but poor specificity (50%) in oral swabs from healthy volunteers. Dried blood spots allow sensitive and specific diagnosis of acute dengue by serological, molecular, and antigen detection methods. Oral swabs may be an adequate alternative where blood cannot be collected.

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Section: Discussionsupporting

confidence: 84%

An Evaluation of Dried Blood Spots and Oral Swabs as Alternative Specimens for the Diagnosis of Dengue and Screening for Past Dengue Virus Exposure

Anders¹,

Nguyet²,

Quyen³

et al. 2012

The American Society of Tropical Medicine and Hygiene

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“…The collection of saliva specimens is not invasive and therefore presents an attractive alternative for the diagnosis of DEN infection. The utility of this type of specimen has been reported for the diagnosis of a number of infectious diseases (6,7,9,20,21), including dengue (1,5). However, earlier studies of the detection of anti-DEN antibodies in saliva were performed on limited numbers of specimens; our study examines over three times more samples than in previous reports.…”

Section: Discussionmentioning

confidence: 99%

Diagnosis of Dengue Virus Infection by Detection of Specific Immunoglobulin M (IgM) and IgA Antibodies in Serum and Saliva

Balmaseda

Guzmán

Hammond

et al. 2003

Clin Vaccine Immunol

129

109

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To evaluate alternative approaches to the serological diagnosis of dengue virus (DEN) infection, the detection of DEN-specific immunoglobulin M (IgM) and IgA antibodies in serum and saliva specimens was assessed in 147 patients with symptoms of DEN infection seen at the Ministry of Health in Nicaragua. Seventy-two serum samples were determined to be positive for anti-DEN antibodies by IgM capture enzymelinked immunosorbent assay, the routine diagnostic procedure. Serum and saliva specimens were obtained from 50 healthy adults as additional controls. IgM was detected in the saliva of 65 of the 72 serum IgM-positive cases, 6 of the 75 serum IgM-negative cases, and none of the control group, resulting in a sensitivity of 90.3% and a specificity of 92.0% and demonstrating that salivary IgM is a useful diagnostic marker for DEN infection. Detection of IgA in serum may be another feasible alternative for the diagnosis of DEN infection, with serum IgA found in 68 (94.4%) of the IgM-positive cases. In contrast, detection of IgA in saliva was not found to be a useful tool for DEN diagnosis in the present study. Further studies of the kinetics of antibody detection in another set of 151 paired acute-and convalescent-phase serum samples showed that DEN-specific IgA antibodies were detected in more acute-phase samples than were IgM antibodies. Thus, we conclude that DEN-specific IgA in serum is a potential diagnostic target. Furthermore, given that saliva is a readily obtainable, noninvasive specimen, detection of DEN-specific salivary IgM should be considered a useful, cheaper diagnostic modality with similar sensitivity and specificity to IgM detection in serum.

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“…[4][5][6][7][8] A few studies have evaluated the use of anti-DENV IgA as a dengue diagnostic marker [9][10][11] ; others have assessed alternatives to serum as diagnostic specimens. 9,[11][12][13] However, no comprehensive studies of multiple immunoglobulin classes and different types of biological samples have been reported, especially in relation to measuring incidence of DENV infection. This study evaluates the utility of different immunological markers for diagnosis of acute illness and for measuring the incidence of DENV infection.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of immunological markers in serum, filter-paper blood spots, and saliva for dengue diagnosis and epidemiological studies

Balmaseda¹,

Saborío²,

Téllez³

et al. 2008

Journal of Clinical Virology

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Diagnosis of dengue infection by detecting specific immunoglobulin M antibodies in saliva samples

Cited by 21 publications

References 17 publications

An Evaluation of Dried Blood Spots and Oral Swabs as Alternative Specimens for the Diagnosis of Dengue and Screening for Past Dengue Virus Exposure

An Evaluation of Dried Blood Spots and Oral Swabs as Alternative Specimens for the Diagnosis of Dengue and Screening for Past Dengue Virus Exposure

Diagnosis of Dengue Virus Infection by Detection of Specific Immunoglobulin M (IgM) and IgA Antibodies in Serum and Saliva

Evaluation of immunological markers in serum, filter-paper blood spots, and saliva for dengue diagnosis and epidemiological studies

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