Diagnosis of Mycoplasma pneumoniae pneumonia: sensitivities and specificities of serology with lipid antigen and isolation of the organism on soy peptone medium for identification of infections

Kenny, George E.; Kaiser, G G; Cooney, Marion K.; Foy, Hjordis M.

doi:10.1128/jcm.28.9.2087-2093.1990

Cited by 89 publications

(35 citation statements)

References 33 publications

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“…For scientific purposes, M. pneumoniae culture is mostly performed with cell‐free formulations such as Hayflick's, New York City, Soy Peptone or SP‐4 medium [42,43]. The last formulation, which had initially been developed for the isolation of Spiroplasma spp., is most effective.…”

Section: Culture Techniquesmentioning

confidence: 99%

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Laboratory diagnosis of Mycoplasma pneumoniae infection

Daxboeck

Krause

Wenisch

2003

Clinical Microbiology and Infection

263

231

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Diagnosis of Mycoplasma pneumoniae infection is challenging due to the fastidious nature of the pathogen, the considerable seroprevalence, and the possibility of transient asymptomatic carriage. During recent years, various new techniques have been adapted for the diagnosis of M. pneumoniae infection, notably in the field of molecular biology. Standard polymerase chain reaction (PCR) is currently the method of choice for direct pathogen detection, but several PCR-related methods provide enhanced sensitivity or more convenient handling procedures, and have been successfully applied for research purposes. Among these techniques are real-time PCR, nested PCR, reverse transcriptase PCR (RT-PCR) and multiplex PCR. Generally, amplification-based methods have replaced hybridization assays and direct antigen detection. Serology, which is the basic strategy for mycoplasma diagnosis in routine clinical practice, has been improved by the widespread availability of sensitive assays for separate detection of different antibody classes. For the diagnosis of mycoplasma pneumonia, serology and direct pathogen detection should be combined. Extrapulmonary diseases may be diagnosed by direct pathogen detection alone, but the value of this diagnostic approach is limited by the probably immunologically mediated pathogenesis of some manifestations. This review summarizes the current state of Mycoplasma pneumoniae diagnosis, with special reference to molecular techniques. The value of different methods for routine diagnosis and research purposes is discussed.

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Section: Culture Techniquesmentioning

confidence: 99%

“…Kenny et al. found sensitivities of 53% and 64% of serology and culture, respectively, when the other approach was regarded as the reference [42]. Similarly, it has been demonstrated that only 58% of patients with positive mycoplasma culture show a four‐fold or greater increase in CF antibody titer [68].…”

Section: Nucleic Acid Amplificationmentioning

confidence: 99%

Laboratory diagnosis of Mycoplasma pneumoniae infection

Daxboeck

Krause

Wenisch

2003

Clinical Microbiology and Infection

263

231

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“…Serologic testing is still primarily used by clinicians in order to establish the diagnosis of Mycoplasma infection; however, negative serologic testing has been seen in PCR confirmed CNS infections [32]. Routine serologic methods used on serum for detection of antibodies against M. pneumoniae include several methods such as complement fixation, immunofluorescence and enzyme immunoassays [83][84][85][86][87][88][89]. Complement fixation is the most widely accepted method [83].…”

Section: Serologymentioning

confidence: 99%

Mycoplasma pneumoniae‐associated myelitis: a comprehensive review

Tsiodras

Kelesidis²,

Kelesidis³

et al. 2006

Euro J of Neurology

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Myelitis is one of the most severe central nervous system complications seen in association with Mycoplasma pneumoniae infections and both acute transverse myelitis (ATM) as well as acute disseminated encephalomyelitis (ADEM) have been observed. We reviewed all available literature on cases of Mycoplasma spp. associated ATM as well as ADEM with dominant spinal cord pathology and classified those cases according to the strength of evidence implicating M. pneumoniae as the cause. A wide range of data on diagnosis, epidemiology, immunopathogenesis, clinical picture, laboratory diagnosis, neuroimaging and treatment for this rare entity is presented. The use of highly sensitive and specific molecular diagnostic techniques may assist in clearly elucidating the role of M. pneumoniae in ATM/ADEM syndromes in the near future. Immunomodulating therapies may have a role in treating such cases.

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“…En effet, plusieurs études ont retrouvé un manque de corrélation entre une PCR positive et la réponse immunitaire avec apparition d'anticorps. Bien que cette discordance apparente soit également connue pour la culture et la sérologie [157], elle demande réflexion. Plusieurs explications à cette discordance entre culture ou PCR positives et absence d'anticorps ont été avancées :…”

Section: Rôle De La Pcr Pour Le Diagnostic Des Infections à M Pneumounclassified

04 – Apport des explorations microbiologiques au diagnostic des infections des voies respiratoires basses

Leroy

2006

Médecine et Maladies Infectieuses

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The diagnosis of community-acquired pneumonia is usually based on clinical and radiological criteria. The identification of a causative organism is not required for the diagnosis. Although numerous microbiological techniques are available, their sensitivity and specificity are not high enough to guide first-line antimicrobial therapy. Consequently, this treatment remains most often empiric. If the causative organism is identified, the antimicrobial treatment is adapted. Sputum analysis may be proposed as a diagnostic tool for patients with an acute exacerbation of chronic obstructive pulmonary disease, in specific cases (prior antibiotherapy, hospitalization, failure of the empiric antimicrobial treatment).

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Diagnosis of Mycoplasma pneumoniae pneumonia: sensitivities and specificities of serology with lipid antigen and isolation of the organism on soy peptone medium for identification of infections

Cited by 89 publications

References 33 publications

Laboratory diagnosis of Mycoplasma pneumoniae infection

Laboratory diagnosis of Mycoplasma pneumoniae infection

Mycoplasma pneumoniae‐associated myelitis: a comprehensive review

04 – Apport des explorations microbiologiques au diagnostic des infections des voies respiratoires basses

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