Diagnóstico da Leishmaniose Visceral Canina: grandes avanços tecnológicos e baixa aplicação prática

Faria, Angélica Rosa; Andrade, Hélida Monteiro de

doi:10.5123/s2176-62232012000200007

Cited by 33 publications

(47 citation statements)

References 81 publications

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“…IFA shows a sensitivity between 83% and 100% and a specificity of approximately 80%. IFA requires specialized, high-cost equipment and trained staff due to the subjective interpretation in the evaluation of the fluorescence intensity by microscopy (EDRISSIAN & DARABIAN, 1979;BARBOSA-DE-DEUS et al, 2002;PALTRINIERI et al, 2010;FARIA & ANDRADE, 2012).…”

Section: Discussionmentioning

confidence: 99%

Variables associated with the prevalence of anti-Leishmania spp. antibodies in dogs on the tri-border of Foz do Iguaçu, Paraná, Brazil

Dias

Soccol

Pasquali

et al. 2018

Rev. Bras. Parasitol. Vet.

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The aim of this study was to investigate the occurrence of anti-Leishmania spp. antibodies in dogs from localities in the city of Foz do Iguaçu, Paraná state, Brazil, on the border with Argentina and Paraguay. Blood samples dogs were collected to perform the following serologic tests: immunochromatographic DPP® rapid test, indirect immunoenzymatic assay (ELISA) and indirect immunofluorescence assay (IFA). In 2012, 285 dogs were analyzed on Argentina border, and in 2013, serum samples from 396 dogs on the border of Paraguay were collected. Using ELISA for screening and IFA for the confirmatory test, the results showed that the antibody prevalence was 1.8% (5/285) on the border of Argentina and 3.0% (12/396) on Paraguay border. When using the DPP® for screening and ELISA as a confirmatory analysis, we observed a seroreagent prevalence in dogs of 2.5% (7/285) on Argentina border and 5.1% (20/396) on Paraguay border. The non-public collection of domestic waste (p= 0.0004) was shown to be associated with leishmaniasis. This study shows the presence of leishmaniasis and suggest the emergence of canine visceral leishmaniasis in state of Paraná due to the confirmed occurrence of seroreactive dogs on Argentina and Paraguay border, which has environmental and geographical characteristics that favor the spread of the parasite.

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Section: Discussionmentioning

confidence: 99%

Variables associated with the prevalence of anti-Leishmania spp. antibodies in dogs on the tri-border of Foz do Iguaçu, Paraná, Brazil

Dias

Soccol

Pasquali

et al. 2018

Rev. Bras. Parasitol. Vet.

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“…Although extensively evaluated with good results in the Old World and Brazil, for the serodiagnosis of L. infantum in dogs, direct agglutination test (DAT) and fast agglutination screening test (FAST) are not used in Brazil 9 - 11 . The Brazilian Ministry of Health distributes a rapid dual-path platform test (TR DPP ® ) for screening, followed by enzyme linked immunosorbent assay (ELISA) for confirmation 12 . However, despite using three recombinant antigens (rK39, K26 and k9, fused together in a recombinant protein called k28) 13 , TR DPP ® sensitivity remains rather disappointing, particularly among sub-clinically infected animals 14 .…”

Section: Introductionmentioning

confidence: 99%

“…Despite their lower sensitivity, laboratory methods considered gold standards for VL confirmation are parasitological approaches 17 . Detection of parasites in tissues, organs or cultures establishes the conclusive diagnosis of L. infantum infection in dogs 12 . However, in addition to the possibility of false-negative results due to the lack of sensitivity, these direct methods are difficult to perform and are invasive.…”

Section: Introductionmentioning

confidence: 99%

The performance of serological tests for Leishmania infantum infection screening in dogs depends on the prevalence of the disease

Mendonça

Batista

Schallig

et al. 2017

Rev. Inst. Med. trop. S. Paulo

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Dogs are considered the main reservoir of Leishmania infantum. This protozoan causes visceral leishmaniasis (VL), an uncontrolled urban zoonosis in Brazil. Serological tests and polymerase chain reaction (PCR) on peripheral blood were performed to identify infected dogs in scenarios of higher and lower prevalence of the disease (Teresina and Vitória). One-hundred infected and 57 non-infected animals from Teresina and 100 non-infected animals from Vitória were studied. Animal selection was not dependent on previous serology. The sensitivity (Teresina) and specificity (Teresina and Vitória) were as follows: indirect antibody fluorescence (IFAT) cut-off of 1:40 (IFAT 1:40): 96%, 18%, and 76%; IFAT 1:80: 90%, 33%, and 93%; direct agglutination test (DAT): 96%, 33%, and 98%; fast agglutination screening test (FAST): 93%, 68%, and 100%; immunochromatographic assay with a recombinant rK39 antigen (rK39): 88%, 74%, and 98%; enzyme linked immunosorbent assay (ELISA): 91%, 79%, and 98%; rapid dual-path platform test (TR DPP®): 98%, 60%, and 98%; and blood PCR: 29%, 93%, and 97%, respectively. In the high transmission area, none of the tests adequately discriminated L. infantum-infected from non-infected dogs. However, in the high transmission city, the area under the receiver operating characteristic (ROC) curve of FAST, DAT, ICrK39, ELISA and TR DPP® was high.

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“…Clinical signs of the dogs that were PCR-negative for leishmaniasis may indicate Ehrlichia infection, because both the parasites show similar clinical signs 7 . Failed serological tests have been reported as false-positives due to cross-reactions with other pathologies, and due to chronic and old infections 7,8 . The Bio-Manguinhos ELISA kits use the promastigotes of Leishmania major, the species that causes cutaneous leishmaniasis, as the antigen, which generates false results.…”

mentioning

confidence: 99%

PCR sensitivity of peripheral blood of dogs co-infected with Leishmania spp. and Ehrlichia spp. in endemic area of Brazil

Silveira

Vieira

Batalini

et al. 2018

Rev. Soc. Bras. Med. Trop.

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Introduction: Peripheral blood of 400 dogs infected with Leishmania and Ehrlichia were analyzed using polymerase chain reaction (PCR), and clinical signs were characterized. Methods: PCR and parasitological tests were conducted. Results: PCR was positive for Leishmania in 84.75%, and parasitological tests showed that 63.25% and 31.75% were positive for Leishmania and Ehrlichia, respectively. All animals showed more than three clinical signs. PCR results were negative for Leishmania in 15.25% of the samples. Conclusions: Conventional PCR of peripheral blood can be used for diagnosing canine visceral leishmaniasis in combination with other techniques, especially in uncertain cases that need species identification.

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Diagnóstico da Leishmaniose Visceral Canina: grandes avanços tecnológicos e baixa aplicação prática

Cited by 33 publications

References 81 publications

Variables associated with the prevalence of anti-Leishmania spp. antibodies in dogs on the tri-border of Foz do Iguaçu, Paraná, Brazil

Variables associated with the prevalence of anti-Leishmania spp. antibodies in dogs on the tri-border of Foz do Iguaçu, Paraná, Brazil

The performance of serological tests for Leishmania infantum infection screening in dogs depends on the prevalence of the disease

PCR sensitivity of peripheral blood of dogs co-infected with Leishmania spp. and Ehrlichia spp. in endemic area of Brazil

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