Diallyl-disulfide, an organosulfur compound of garlic, attenuates airway inflammation via activation of the Nrf-2/HO-1 pathway and NF-kappaB suppression

Shin, In‐Sik; Hong, Ju-Mi; Jeon, Chan‐Mi; Shin, Na‐Rae; Kwon, Ok‐Kyoung; Kim, Hui‐Seong; Kim, Jong-Choon; Oh, Sei‐Ryang; Ahn, Ki Hoon

doi:10.1016/j.fct.2013.09.012

Cited by 91 publications

(66 citation statements)

References 21 publications

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“…The protective effect of DADS on reducing ROS and MDA levels and enhancing Nrf2 nuclear translocation in current study are in agreement with two previous studies indicating that DADS effectively reduces ROS and NO production in murine macrophages exposed to LPS in vitro and ovalbumin‐induced asthmatic mice in vivo. These effects have been attributed to elevated Nrf2 nuclear translocation in both in vivo and in vitro [Shin et al, ].…”

Section: Discussionmentioning

confidence: 99%

“…DADS has various pharmacological properties, such as antioxidant, anti‐inflammatory, and anti‐apoptotic [Sheen et al, ; Lee et al, ]. Recent studies have shown that anti‐inflammatory effects of DADS is due to inhibition of nuclear factor‐κB (NF‐κB) pathway and subsequent reduction in expression of pro‐inflammatory cytokines including TNF‐α, IL‐1β as well as inducible nitric oxide synthase (iNOS) and cyclooxygenase‐2 (COX‐2) [Woo et al, ; Park et al, ; Shin et al, ; Schafer and Kaschula, ; Saud et al, ]. DADS has also shown to be able to reduce MMPs expression following stimulation of chondrosarcoma cells with IL‐1β [Williams et al, ].…”

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confidence: 99%

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Evaluating the Protective Effects and Mechanisms of Diallyl Disulfide on Interlukin‐1β‐Induced Oxidative Stress and Mitochondrial Apoptotic Signaling Pathways in Cultured Chondrocytes

Hosseinzadeh

Jafari

Kamarul

et al. 2017

J of Cellular Biochemistry

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The protective effects and mechanisms of DADS on IL-1β-mediated oxidative stress and mitochondrial apoptosis were investigated in C28I2 human chondrocytes. The effect of various concentrations of DADS (1, 5 10, 25, 50, and 100 μM) on C28I2 cell viability was evaluated in different times (2, 4, 8, 16, and 24 h) to obtain the non-cytotoxic concentrations of drug by MTT-assay. The protective effect of non-toxic concentrations of DADS on experimentally induced oxidative stress and apoptosis by IL-1β in C28I2 was evaluated. The effects of DADS on IL-1β-induced intracellular ROS production and lipid peroxidation were detected and the proteins expression of Nrf2, Bax, Bcl-2, caspase-3, total and phosphorylated JNK, and P38 MAPKs were analyzed by Western blotting. The mRNA expression of detoxifying phase II/antioxidant enzymes including heme oxygenase-1, NAD(P)H quinine oxidoreductase, glutathione S-transferase-P1, catalase, superoxide dismutase-1, glutathione peroxidase-1, -3, -4 were evaluated by reverse transcription-polymerase chain reaction. DADS in 1, 5, 10, and 25 μM concentrations had no cytotoxic effect after 24 h. Pretreatment with DADS remarkably increased Nrf2 nuclear translocation as well as the genes expression of detoxifying phase II/antioxidant enzymes and reduced IL-1β-induced elevation of ROS, lipid peroxidation, Bax/Bcl-2 ratio, caspase-3 activation, and JNK and P38 phosphorylation. DADS could considerably reduce IL-1β-induced oxidative stress and consequent mitochondrial apoptosis, as the major mechanisms of chondrocyte cell death in an experimental model of osteoarthritis. It may be considered as natural product in protecting OA-induced cartilage damage in clinical setting. J. Cell. Biochem. 118: 1879-1888, 2017. © 2017 Wiley Periodicals, Inc.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Evaluating the Protective Effects and Mechanisms of Diallyl Disulfide on Interlukin‐1β‐Induced Oxidative Stress and Mitochondrial Apoptotic Signaling Pathways in Cultured Chondrocytes

Hosseinzadeh

Jafari

Kamarul

et al. 2017

J of Cellular Biochemistry

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“…Diallyl disulfide and diallyl trisulfide (DATS) are organic sulfur compounds found in members of the Allium species (garlic, onions, chives, etc.) that act as H 2 S donors and have antioxidant, anti-inflammatory, and vasorelaxant properties (14,94,116,132). Slow release sulfide donors such as GYY4137 (88), SG1002 (9,78,118), AP39 (138), and S-propargyl-cysteine (58,149) (118).…”

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confidence: 99%

Emerging role of hydrogen sulfide-microRNA crosstalk in cardiovascular diseases

Hackfort

Mishra

2016

American Journal of Physiology-Heart and Circulatory Physiology

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“…In addition, binding of the activated NF-kB to a unique DNA sequence in the iNOS promoter is crucial for the regulation of the iNOS gene expression [21]. Although the influence of the three OSC on the down-regulation of LPS-induced NF-kB activation was not investigated in this study, many reports noted that DADS has been shown to effectively reduce the expression of proinflammatory proteins including iNOS, NF-kB, and matrix metalloproteinase (MMP-9), and it enhances the expression of antioxidant proteins including Nrf-2 (nuclear factor E2-related factor 2) and hemeoxygenase (HO-1) [28]. It was found that LPS-induced iNOS and COX-2 expression in RAW 264.7 macrophage cells was inhibited by the three OSC.…”

Section: Resultsmentioning

confidence: 99%

The Anti-Inflammatory and Vasodilating Effects of Three Selected Dietary Organic Sulfur Compounds from Allium Species

Chu

Shieh

et al. 2017

JFB

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The anti-inflammatory and vasodilating effects of three selected dietary organic sulfur compounds (OSC), including diallyl disulfide (DADS), dimethyl disulfide (DMDS), and propyl disulfide (PDS), from Allium species were investigated. In the anti-inflammatory activity assay, the three OSC demonstrated significant inhibition of nitric oxide (NO) and prostaglandin E2 (PGE2) production in LPS-induced RAW 264.7 cells. The expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX-2) in activated RAW 264.7 cells was inhibited by the three OSC, indicating that the three OSC prevented the LPS-induced inflammatory response in RAW 264.7 cells. For the vasodilative assay, the three OSC were ineffective in producing NO in SVEC4-10 cells, but they did enhance prostacyclin (PGI2) production. The expression of COX-2 in SVEC4-10 cells was activated by DADS and DMDS. Pretreatment of SVEC4-10 cells with the three OSC decreased ROS generation in H2O2-induced SVEC4-10 cells. In addition, the three OSC significantly inhibited angiotensin-I converting enzyme (ACE). The up-regulation of PGI2 production and COX-2 expression by DADS and DMDS and the reduction of ROS generation by DADS, DMDS, and PDS in SVEC4-10 cells contributed to the vasodilative effect of the three OSC. Collectively, these findings suggest that DADS, DMDS, and PDS are potential anti-inflammatory and vasodilative mediators.

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Diallyl-disulfide, an organosulfur compound of garlic, attenuates airway inflammation via activation of the Nrf-2/HO-1 pathway and NF-kappaB suppression

Cited by 91 publications

References 21 publications

Evaluating the Protective Effects and Mechanisms of Diallyl Disulfide on Interlukin‐1β‐Induced Oxidative Stress and Mitochondrial Apoptotic Signaling Pathways in Cultured Chondrocytes

Evaluating the Protective Effects and Mechanisms of Diallyl Disulfide on Interlukin‐1β‐Induced Oxidative Stress and Mitochondrial Apoptotic Signaling Pathways in Cultured Chondrocytes

Emerging role of hydrogen sulfide-microRNA crosstalk in cardiovascular diseases

The Anti-Inflammatory and Vasodilating Effects of Three Selected Dietary Organic Sulfur Compounds from Allium Species

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