Die Klärungsfähigkeit des Blutplasmas und Veränderungen des Plasmacholesterinspiegels nach intravenöser Heparininjektion bei Diabetikern und Atherosklerotikern

Stork, A; Kucerová, L

doi:10.1055/s-0028-1116896

Cited by 8 publications

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“…been intensively studied by many investigators as related to atherosclerosis. Some (12)(13)(14)(15)(16)(17) observed lower LPL activity in patients with atherosclerosis, but others (8,(18)(19)(20)(21)(22) found no difference in LPL activity between normal and atherosclerotic subjects. Our results showed that the peak values were the same in both groups.…”

Section: Resultsmentioning

confidence: 99%

Kinetic Studies on Metabolism of Lipoprotein Lipase*

Yoshitoshi¹,

Naito²,

Okaniwa³

et al. 1963

J. Clin. Invest.

106

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Since Hahn demonstrated the clearing of alimentary lipemia by the intravenous injection of heparin, many excellent studies have been done on lipoprotein lipase (LPL). In these studies, however, LPL activities were compared in normal and pathological conditions at a certain time after heparin injection. If the level of LPL activity in the circulating blood changes continuously after heparin injection, under the influences of production and inactivation of LPL, it may be much more useful, under certain circumnstances, to measure the rate of change as well as the amount of such activity.One of the authors reported previously (1) the method of estimating the release or production rate and inactivation rate of LPL after heparin injection. This report will present the data on the metabolism of LPL activity in blood after the intravenous injection of heparin in normal subjects and patients with liver diseases, obesity, and coronary sclerosis, and in some animals. EXPERIMENTAL METHODSPreparation of materials. Human subjects and experimental animals were fasted overnight before the experiment. Experimental animals (dogs and rabbits) were anesthetized with pentobarbital sodium (Nembutal) just before use.Heparin injection. The doses of heparin injected were 0.1 mg per kg of body weight throughout the experiment. The injection was done rapidly into the antecubital vein in men and dogs and into the ear vein in rabbits.Withdrawals of blood. At certain intervals after the injection, blood was withdrawn from the antecubital vein in human subj ects, from the femoral vein in dogs, and by heart puncture in rabbits. The blood specimen was immediately added to 1/9 vol of Y/10 M sodium oxalate solution kept in an ice-water bath.Assay of LPL activity. The test plasma weas obtained by centrifuging the blood for 5 minutes at 4,000 rpm in the cold. The substrate consisted of 0.1 vol of 2.5%o * Supported in part by a grant-in-aid for research from the Ministry of Education and a grant-in-aid for atherosclerosis study from the Ministry of \Velfare. Immediately after the mixing of 1 ml of test plasma with 1.5 ml of substrate, 1.0 ml of the mixture was pipetted into Dole's extraction mixture, and the remainder was incubated at 390 C for 30 minutes. The LPL activity was expressed by the difference of FFA concentrations (microequivalents per liter) in the mixture before and after the incubation. FFA was measured by Dole's method (2). The details of our method were discussed in the previous paper (1). RESULTS AND DISCUSSIONLPL activity curve in normnal human beings, dogs, and rabbits. Eight healthy men aged 25 to 35 years, 4 dogs, and 3 rabbits were used. Figures 1 and 2 show the changes of LPL activity as a function of time in human subjects and dogs, respectively. Similar changes of LPL activity were observed also in rabbits. The rapid rise in LPL activity was observed immediately after heparin injection, and the peak was after about 9 minutes in human subjects, about 8 minutes in dogs, and about 3 minutes in rabbits. Thereafter, the activity...

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Section: Resultsmentioning

confidence: 99%

Kinetic Studies on Metabolism of Lipoprotein Lipase*

Yoshitoshi¹,

Naito²,

Okaniwa³

et al. 1963

J. Clin. Invest.

106

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Cholesterin‐Stoffwechsel bei Atherosklerose und seine pharmakodynamische Beeinflussung

Kraupp

1961

Fette, Seifen, Anstrichm.

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Die Frage nach einem Kausalzusammenhang zwischen einem gestorten Cholesterin-Stoffwechsel bzw. Gesamtlipoid-Stoffwechsel und der Entstehung atheromatoser GefaRveranderungen wird behandelt. Die pharmakodynamischen Wirkungen, die sich u. a. auf die Hemmung der endogenen Cholesterin-Synthese, die Verhinderung der Ablagerung von Cholesterin in der Gefanwand beziehen, werden erortert. Cholesterol Metabolism during Atherosclerosis and its Pharmacodynamic InfluenceThe question according to a causative relationship between a disturbed cholesterol-metabolism as well as the total lipid-metabolism and the formation of atherosclerotic vessel changes, has been treated. The pharmaco-dynamic action, which under others, relates to the stoppage of the endogenic syntheses of cholesterol and to the hindrance of the deposition of cholesterol on the vessel wall, have been discussed.Zwei Befunde haben Cholesterin bzw. dessen Stoffwechsel in einen pathogenetischen Zusammenhang mit der Atherosklerose gebracht: Der Nachweis von Cholesterin in der Intima atheromatoser Gefai3e (L. Aschof'; A . Windaus z, und die experimentelle Erzeugung einer Atheromatose durch Cholesterin-Verfiitterung an Herbivoren ( N . Ani~schkow ". Seit dieser Zeit steht die Frage nach einem eventuellen Kausalzusammenhang zwischen einem gestorten Cholesterin-Stoffwechsel und der Entstehung atheromatoser Gefaflveranderungen im Mittelpunkt der Forschung. Hierbei wurde sowohl deduktiv statistisch als auch induktiv analysierend vorgegangen. Von den Ergebnissen der methodisch deduktiven Forschung sind zunachst jene von Interesse, die die Haufigkeit des Auftretens einer Atherosklerose bei primaren Storungen des Cholesterin-bzw. Gesamtlipoid-Stoffwechsels betreffen. Die Einbeziehung des letzteren erwies sich als notwendig, dawie weiter unten noch naher erlautert wirdisolierte Storungen des Cholesterin-Stoffwechsels sehr selten sind und in der Regel mit dem Cholesterin auch alle iibrigen Lipoid-Fraktionen pathologische Veranderungen zeigen. Ober die A t h e r o s k l e r o s e b e i i d i o p a t h is c h e r H y p e r c h o l e s t e r i n a m i e u n d L i p o i da m i e liegen zusammenfassende Studien aus jiingster Zeit vor (D. Adlersberg und L. E . Schaefer4; 1. L. Gu-raviclz5; H . F. Epstein und Mitarbb. E; G. Schettler und Mitarbb.'), aus denen hervorgeht, dal3 in Familien rnit eindeutiger erblicher 13elastung hinsichtlich der 0. a. Stoffwechsel-Storungen in etwa 40 Oio eine manifeste Coronar-Erkrankung und durch Verfeinerung der Unter-". Vortrag anlal3lich der DGF-Vortragstagung in Hamburg am 26. Oktober 1960. Le metabolisme du cholest6rol dans I'ath6roscl6rose et son influence pharmacodynamique On traite de la question s'il existe une relation causale entre un metabolisme du cholesterol ou un metabolisme lipidique total deregles et l'apparition d e transformations atheroinateuses des vaisseaux sanguins. On discute des effets pharniacodynamiques qui empechent la formation d e depBts d e cholesterol sur les parois des vaisseaux, et entre autre qui ralentissent la synthe...

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Die Lipoproteidlipase und ihre klinische Bedeutung

Schöll¹,

Schettler²

1961

Ergebnisse Der Inneren Medizin Und Kinderheilkunde

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Die Klärungsfähigkeit des Blutplasmas und Veränderungen des Plasmacholesterinspiegels nach intravenöser Heparininjektion bei Diabetikern und Atherosklerotikern

Cited by 8 publications

References 0 publications

Kinetic Studies on Metabolism of Lipoprotein Lipase*

Kinetic Studies on Metabolism of Lipoprotein Lipase*

Cholesterin‐Stoffwechsel bei Atherosklerose und seine pharmakodynamische Beeinflussung

Die Lipoproteidlipase und ihre klinische Bedeutung

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