Dielectrophoretic Manipulation and Separation of Microparticles Using Microarray Dot Electrodes

Abstract

This paper introduces a dielectrophoretic system for the manipulation and separation of microparticles. The system is composed of five layers and utilizes microarray dot electrodes. We validated our system by conducting size-dependent manipulation and separation experiments on 1, 5 and 15 μm polystyrene particles. Our findings confirm the capability of the proposed device to rapidly and efficiently manipulate and separate microparticles of various dimensions, utilizing positive and negative dielectrophoresis (DEP) effects. Larger size particles were repelled and concentrated in the center of the dot by negative DEP, while the smaller sizes were attracted and collected by the edge of the dot by positive DEP.

Dielectrophoretic behavior of PEGylated RNase A inside a microchannel with diamond‐shaped insulating posts

“…iDEP has been used for manipulation and separation of inert microparticles to a great extent . Manipulation of bioparticles such as bacteria , algae , mammalian cells , sperm cells , and DNA has also been reported.…”

Microfluidic device embedding electrodes for dielectrophoretic manipulation of cells‐A review

“…Figure illustrates the design and operation of the dielectrophoresis device used in the current work, which was introduced and validated in . The microarray dot electrodes were fabricated by using standard photolithography process.…”

“…(A) Dielectrophoresis device layers and a close‐up view of the 4 × 4 microarray dot electrode, with cells distributed homogenously in the microchannel provided by the microfluidic gasket. (B) Cells distributed homogenously over the dot area before the electrical signal is applied (1), cells experiencing the positive dielectrophoresis effect and thus being attracted to the dot electrode edge (2), and cells collected at the dot center under the effect of negative dielectrophoresis (3) .…”

“…This class of microfluidic device with microarray dot electrodes illustrates the feasibility of dielectrophoresis to be used in detecting dengue virus; however, clinical investigation is needed to assess the sensitivity of this technique compared to the published methods .…”

Assessing the importance of the root mean square (RMS) value of different waveforms to determine the strength of a dielectrophoresis trapping force

“…We characterized the differences in particle trapping when using waveforms with a given frequency and either equivalent peak‐to‐peak voltages (V pp ), equivalent wave energy (E x ) or equivalent RMS voltage (V RMS ). The frequency range of 5–50 kHz was used for this work due to previous work showing pDEP for 1.1 μm polystyrene beads . The Clausius‐Mossotti factor for these experiments decreased by 0.05 across this frequency range.…”