Dielektrophorese als Grundlage für ein neues Verfahren zur präparativen Zelltrennung

Gläser, Roland; Pescheck, C; Krause, G.; Schmidt, K. P.; Teuscher, L.

doi:10.1002/jobm.19790190902

J of Basic Microbiology

1979

DOI: 10.1002/jobm.19790190902

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Dielektrophorese als Grundlage für ein neues Verfahren zur präparativen Zelltrennung

Roland Gläser¹,

C Pescheck²,

G. Krause³

et al.

Abstract: Dielektrophorese ist die Bewegung polarisierbarer Teilchen in einem inhomogenen elektrischen Wechselfeld in Richtung steigender Feldstärke. Entsprechend der Polarisierbarkeit der Partikeln ist dieser Effekt frequenzabhängig. Es wird eine präparative Trennkammer beschrieben, die auf folgendem Prinzip beruht: In einem Elektrodenraum sammeln sich Zellen durch den Dielektrophorese‐effekt an einer koaxial gelegenen Drahtelektrode. Dieser Draht wird mit konstanter Geschwindigkeit bewegt, so daß die angehefteten Zell… Show more

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1981

1982

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Cited by 3 publications

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Microbiological implications of electric field effects III. Stimulation of yeast protoplast fusion by electric field pulses

Weber

Förster

Jacob

et al. 1981

J of Basic Microbiology

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Prototrophic colonies could be selected on minimal medium after mixing of protoplasts from diauxotrophic mutants of t h e yeasts Saeeharomycopsis lipolytiea and/or Lodderomyees elongisporus and treatment with polyethylene glycol (PEG) in the presence of calcium chloride. This is the result of protoplast fusion and complementation of auxotrophic deficiencies. Under identical conditions a n electric field puke in t h e ps-range applied via an electric discharge t o t h e protoplast-PEG mixture resulted in a drastic enhancement of the protoplast fusion rate. The presence of polyethylene glycol was demonstrated to be a prerequisite for fusion in this case, too. T h e frequency of hybrid formation detected as prototrophic colonies could be increased in t h e case of intraspecific fusion at initial electric field strengths between 2.5 and 5 kV . cm-l. The application of an electric field pulse of proper strength and duration to a yeast protoplast suspension turned out to be a more effective tool in production of fusion products than conventional methods. Largs numbers of parasexual hybrids for different selection programmes in yeast genetics and for induetrial purpose may be delivered by this technique. Taking into account this knowledge we started in 1978 with experiments on protoplast fusion in the PEG-Ca2+ system in order t o try to enhance thc fusion frequency of different yeast species by electric field pulse treatment. First positive results we of 10-5 to 10-4.

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Microbiological implications of electric field effects III. Stimulation of yeast protoplast fusion by electric field pulses

Weber

Förster

Jacob

et al. 1981

J of Basic Microbiology

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Anwendung der Dielektrophorese für die präparative Trennung thermotoleranter Hefezellen

Krause

Schade

Gläser

et al. 1982

J of Basic Microbiology

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Dielectrophoresis as a new method for preparative separation of cells is being present5d. This method responds to differences in the polarization of cells, which correlate with a number of complex physiological properties of cells. It is shown t h a t yeast cells of different thermotolerant properties can be differentiated by dielectrophoresis. Moreover, the application of this method permitted to separate cells with a high selectivity, which produce more biomass a t a temperature of 40 "C than the initial population.Investigations on the ability of cells to survive in an inhomogeneous a. c. field have shown that the number of dead cells a t frequencies of lo5 cps and 106 cps and a t field strengths (on the surface of the central electrode) below 2.4 x lo5 V/m is small. This fact should be considered when picking out suitable separation conditions.

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Dielektrophoretische Untersuchungen an biologischen Systemen

Lamprecht

Mischel

1981

Naturwissenschaften

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Dielektrophorese als Grundlage für ein neues Verfahren zur präparativen Zelltrennung

Cited by 3 publications

References 8 publications

Microbiological implications of electric field effects III. Stimulation of yeast protoplast fusion by electric field pulses

Microbiological implications of electric field effects III. Stimulation of yeast protoplast fusion by electric field pulses

Anwendung der Dielektrophorese für die präparative Trennung thermotoleranter Hefezellen

Dielektrophoretische Untersuchungen an biologischen Systemen

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