30Objectives: Gender differentiation can influence the diet, physical activity, and health of human 31 populations. Multifaceted approaches are therefore necessary when exploring the biological 32 consequences of gender-related social norms in the past. Here, we explore the links between diet, 33 physiological stress, physical activity and gender differentiation in the Medieval Islamic 34 population of La Torrecilla (Granada, Spain, 13 th -15 th century AD), by analyzing stable isotope 35 patterns, stature, and long bone diaphyseal measurements. 36 Materials and Methods: The sample includes 96 individuals (48 females, 48 males) classified 37 as young and middle adults (20-34 and 35-50 years of age respectively). Diet was reconstructed 38 through the analysis of δ 13 C and δ 15 N. Stature, humeral and femoral diaphyseal shape and 39 product of diaphyseal diameters served as proxies of physiological stress and physical activity.40 Results: Isotopic ratios suggest a substantial dietary contribution of C 4 plants (e.g., sorghum, 41 millet), a variable access to animal proteins, and no differences between the sexes. Sexual 42 dimorphism in stature derives from a markedly low female stature. Long bone diaphyseal 43 properties suggest that men performed various physically stressful activities, whereas women 44 were involved in less physically demanding activities (possibly related to household work).45 Discussion: Gender differentiation in La Torrecilla was expressed by a possibly differential 46 parental investment in male versus female offspring and by culturally sanctioned gender 47 differences in the performance of physical tasks. Diet was qualitatively homogenous between the 48 sexes, although we cannot rule out quantitative differences. Our results shed new light on the 49 effects of gender-related social norms on human development and lifestyle.