Dietary genistein increases microbiota-derived short chain fatty acid levels, modulates homeostasis of the aging gut, and extends healthspan and lifespan

Hou, Qihang; Huang, Jinguang; Zhao, Ling; Pan, Xianjie; Liao, Chaoyong; Jiang, Qingwei; Lei, Jiaqi; Guo, Fangshen; Cui, Jian; Guo, Yuming; Zhang, Bingkun

doi:10.1016/j.phrs.2023.106676

Cited by 42 publications

(33 citation statements)

References 77 publications

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“…The observed fluctuations in the fecal concentrations of butyrate and propionate between the two groups – those in the FF and FFL groups – supported our argument. 39,40 SCFAs play a crucial role beyond being the primary energy source for colonocytes. They promote epithelial cell proliferation and strengthen intestinal barrier function by regulating tight junctions and modulating the AMP-activated protein kinase (AMPK) pathway.…”

Section: Discussionmentioning

confidence: 99%

Effect of Lactobacillus plantarum BFS1243 on a female frailty model induced by fecal microbiota transplantation in germ-free mice

Dong,

Zeng,

et al. 2024

Food Funct.

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Section: Discussionmentioning

confidence: 99%

Effect of Lactobacillus plantarum BFS1243 on a female frailty model induced by fecal microbiota transplantation in germ-free mice

Dong,

Zeng,

et al. 2024

Food Funct.

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“…However, a significant decrease in SCFAs was also found in the He group compared to the beginning of the trial, which may be related to the lower carbohydrate and higher fat content in the diet. It has been shown that SCFAs can improve the inflammatory response of the body, induced by a high-fat diet [79,80], where butyric acid alleviates both oxidative stress and inflammation [81]. In contrast, SCFA deficiency leads to increased intestinal permeability, which triggers an inflammatory cascade response and induces the development of inflammatory diseases [82].…”

Section: Discussionmentioning

confidence: 99%

Different Diet Energy Levels Alter Body Condition, Glucolipid Metabolism, Fecal Microbiota and Metabolites in Adult Beagle Dogs

Sun

Zhang

et al. 2023

Metabolites

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Diet energy is a key component of pet food, but it is usually ignored during pet food development and pet owners also have limited knowledge of its importance. This study aimed to explore the effect of diet energy on the body condition, glucolipid metabolism, fecal microbiota and metabolites of adult beagles and analyze the relation between diet and host and gut microbiota. Eighteen healthy adult neutered male beagles were selected and randomly divided into three groups. Diets were formulated with three metabolizable energy (ME) levels: the low-energy (Le) group consumed a diet of 13.88 MJ/kg ME; the medium-energy (Me) group consumed a diet of 15.04 MJ/kg ME; and the high-energy (He) group consumed a diet of 17.05 MJ/kg ME. Moreover, the protein content of all these three diets was 29%. The experiment lasted 10 weeks, with a two-week acclimation period and an eight-week test phase. Body weight, body condition score (BCS), muscle condition score (MCS) and body fat index (BFI) decreased in the Le group, and the changes in these factors in the Le group were significantly higher than in the other groups (p < 0.05). The serum glucose and lipid levels of the Le and He groups changed over time (p < 0.05), but those of the Me group were stable (p > 0.05). The fecal pH of the Le and He groups decreased at the end of the trial (p < 0.05) and we found that the profiles of short-chain fatty acids (SCFAs) and bile acids (BAs) changed greatly, especially secondary BAs (p < 0.05). As SCFAs and secondary BAs are metabolites of the gut microbiota, the fecal microbiota was also measured. Fecal 16S rRNA gene sequencing found that the Me group had higher α-diversity indices (p < 0.05). The Me group had notably higher levels of gut probiotics, such as Faecalibacterium prausnitzii, Bacteroides plebeius and Blautia producta (p < 0.05). The diet–host–fecal microbiota interactions were determined by network analysis, and fecal metabolites may help to determine the best physical condition of dogs, assisting pet food development. Overall, feeding dogs low- or high-energy diets was harmful for glucostasis and promoted the relative abundance of pathogenic bacteria in the gut, while a medium-energy diet maintained an ideal body condition. We concluded that dogs that are fed a low-energy diet for an extended period may become lean and lose muscle mass, but diets with low energy levels and 29% protein may not supply enough protein for dogs losing weight.

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“…Western blotting for differentially expressed proteins was performed according to a previously reported method. − Cells were lysed with RIPA buffer (Thermo Fisher Scientific, Waltham, USA) containing protease and phosphatase inhibitors (Thermo Fisher Scientific, Waltham, USA). Protein concentrations were determined using a BCA protein assay kit (Thermo Fisher Scientific, Waltham, USA).…”

Section: Methodsmentioning

confidence: 99%

Dual-sgRNA CRISPRa System for Enhanced MK-7 Production and Salmonella Infection Mitigation in Bacillus subtilis natto Applied to Caco-2 Cells

Liao,

Cui,

Gao

et al. 2024

J. Agric. Food Chem.

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This study optimized the menaquinone-7 (MK-7) synthetic pathways in Bacillus subtilis (B. subtilis) natto NB205, a strain that originated from natto, to enhance its MK-7 production. Utilizing mutation breeding, we developed NBMK308, a mutant strain that demonstrated a significant 117.23% increase in MK-7 production. A comprehensive transcriptome analysis identified two key genes, ispA and ispE, as being critical in MK-7 synthesis. The dual-sgRNA CRISPRa system was utilized to achieve precise regulation of ispA and ispE in the newly engineered strain, A3E3. This strategic modulation resulted in a significant enhancement of MK-7 production, achieving increases of 20.02% and 201.41% compared to traditional overexpression systems and the original strain NB205, respectively. Furthermore, the fermentation supernatant from A3E3 notably inhibited Salmonella invasion in Caco-2 cells, showcasing its potential for combating such infections. The safety of the dual-sgRNA CRISPRa system was confirmed through cell assays. The utilization of the dual-sgRNA CRISPRa system in this study was crucial for the precise regulation of key genes in MK-7 synthesis, leading to a remarkable increase in production and demonstrating additional therapeutic potential in inhibiting pathogenic infections. This approach effectively combined the advantages of microbial fermentation and biotechnology, addressing health and nutritional challenges.

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Dietary genistein increases microbiota-derived short chain fatty acid levels, modulates homeostasis of the aging gut, and extends healthspan and lifespan

Cited by 42 publications

References 77 publications

Effect of Lactobacillus plantarum BFS1243 on a female frailty model induced by fecal microbiota transplantation in germ-free mice

Effect of Lactobacillus plantarum BFS1243 on a female frailty model induced by fecal microbiota transplantation in germ-free mice

Different Diet Energy Levels Alter Body Condition, Glucolipid Metabolism, Fecal Microbiota and Metabolites in Adult Beagle Dogs

Dual-sgRNA CRISPRa System for Enhanced MK-7 Production and Salmonella Infection Mitigation in Bacillus subtilis natto Applied to Caco-2 Cells

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