Dietary infection of Enterobacter ludwigii causes fat accumulation and resulted in the diabetes-like condition in Drosophila melanogaster

Priyadarsini, Subhashree; Mukherjee, Sumit; Samikshya, S.N.; Bhanja, Amrita; Paikra, Sanjeev Kumar; Nayak, Nibedita; Mishra, Monalisa

doi:10.1016/j.micpath.2020.104276

Cited by 11 publications

(13 citation statements)

References 79 publications

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“… 20 In a study of dietary infection of Enterobacter ludwigii to fly, a diabetes‐like condition, such as elevated glucose level and increased amount of lipid, was promoted due to the absences of production of short‐chain fatty acid (SCFA) of the bacteria. 21 These characteristics may have contributed to the positively or negatively correlated tendency between the genus and the antihyperglycemic effect. On the other hand, several studies have reported that Erysipelatoclostridium are not beneficial.…”

Section: Discussionmentioning

confidence: 99%

“…Intestinal infusion of Escherichia coli protein stimulates the secretion of plasma peptide YY (PYY), which is the gut satiety hormone, and inhibits food intake in mouse and rat models, which implies a beneficial role for antihyperglycemic effect 20 . In a study of dietary infection of Enterobacter ludwigii to fly, a diabetes‐like condition, such as elevated glucose level and increased amount of lipid, was promoted due to the absences of production of short‐chain fatty acid (SCFA) of the bacteria 21 . These characteristics may have contributed to the positively or negatively correlated tendency between the genus and the antihyperglycemic effect.…”

Section: Discussionmentioning

confidence: 99%

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Effects of vancomycin‐induced gut microbiome alteration on the pharmacodynamics of metformin in healthy male subjects

Kim

Lee

et al. 2021

Clinical Translational Sci

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Metformin is a major treatment for type 2 diabetes. This study was conducted to investigate the impact of gut microbiome dysbiosis on the pharmacokinetics and anti-hyperglycaemic effects of metformin.Healthy adult males aged 19-45 with no defecation abnormalities were recruited for this four-period clinical study: baseline; post-metformin (i.e., multiple oral doses of 1000 mg metformin on days 1-4); post-vancomycin (i.e., multiple oral doses of 500 mg vancomycin on days 11-17 inducing gut microbiome changes); post-metformin+vancomycin (i.e., multiple oral doses of 1000 mg metformin on days 16-19). In each period, serum glucose and insulin concentrations following an oral glucose tolerance test, faecal samples for gut microbiome composition, and safety data were obtained. Following metformin dosing, plasma and urine samples for pharmacokinetics were collected.Nine subjects completed the study. The pharmacokinetics of metformin remained unchanged, and antihyperglycaemic effect was significantly decreased after vancomycin administration (p-value=0.039), demonstrating the weak relationship between the pharmacokinetics and pharmacodynamics of metformin. Relative abundances of some genus were changed after vancomycin administration, and tended to correlate with the anti-hyperglycaemic effects of metformin (p-value=0.062 for Erysipelatoclostridium; p-value=0.039 for Enterobacter; p-value=0.086 for Faecalibacterium). Adverse events occurred in all subjects and were resolved without sequelae.In conclusion, a decrease in the anti-hyperglycaemic effect of metformin was observed after concomitant administration with vancomycin, without changes in metformin pharmacokinetics. The anti-hyperglycaemic effect was tended to correlate with the relative abundance of several genus, suggesting that the effect of metformin is partly attributable to the gut microbiome (ClinicalTrials.gov, NCT03809260).

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effects of vancomycin‐induced gut microbiome alteration on the pharmacodynamics of metformin in healthy male subjects

Kim

Lee

et al. 2021

Clinical Translational Sci

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“…They were nurtured under optimum conditions of 60% relative humidity, 25 °C constant temperature, and 12 h day− night condition. 22,23 At first, a stock solution of 2.5 mM concentration of MnFe 2 O 4 @ poly(tBGE-alt-PA) was made by mixing the nanocomposite in powdered form in Mili Q water (7732185, SRL Chemicals, India), and the solution was stored at 4 °C. Fly food was prepared and divided into control and treatment.…”

Section: Introductionmentioning

confidence: 99%

In Vivo Toxicological Analysis of MnFe₂O₄@poly(tBGE-alt-PA) Composite as a Hybrid Nanomaterial for Possible Biomedical Use

Kumar

Bauri

Sahu

et al. 2023

ACS Appl. Bio Mater.

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Nanocomposites have significantly contributed to biomedical science due to less aggregation behavior and enhanced physicochemical properties. This study synthesized a MnFe2O4@poly(tBGE-alt-PA) nanocomposite for the first time and physicochemically characterized it. The obtained hybrid nanomaterial was tested in vivo for its toxicological properties before use in drug delivery, tissue engineering fields, and environmental applications. The composite was biocompatible with mouse fibroblast cells and hemocompatible with 2% RBC suspension. This nanocomposite was tested on Drosophila melanogaster due to its small size, well-sequenced genome, and low cost of testing. The larvae’s crawling speed and direction were measured after feeding. No abnormal path and altered crawling pattern indicated the nonappearance of abnormal neurological disorder in the larva. The gut organ toxicity was further analyzed using DAPI and DCFH-DA dye to examine the structural anomalies. No apoptosis and necrosis were observed in the gut of the fruit fly. Next, adult flies were examined for phenotypic anomalies after their pupal phases emerged. No defects in the phenotypes, including the eye, wings, abdomen, and bristles, were found in our study. Based on these observations, the MnFe2O4@poly(tBGE-alt-PA) composite may be used for various biomedical and environmental applications.

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“…Therefore, the stabilization of intestinal microflora is critical to intestinal health, barrier function, and nutrient absorption ( Mohebodini et al., 2019 ). Enterobacter is involved in fat accumulation and lipid metabolism ( Ferreira Barletta et al., 2016 ; Priyadarsini et al., 2020 ). Enterococcus faecium belongs to Lactobacillus , and it is a normal beneficial bacterium in the gastrointestinal tract of mammals ( Zhao et al., 2013 ; Castaneda et al., 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Comparison of overfeeding effects on gut physiology and microbiota in two goose breeds

Wei

et al. 2021

Poultry Science

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To have a better understanding of how the “gut–liver axis” mediates the lipid deposition in the liver, a comparison of overfeeding influence on intestine physiology and microbiota between Gang Goose and Tianfu Meat Goose was performed in this study. After force-feeding, compared with Gang Goose, Tianfu Meat Goose had better fat storage capacity in liver (397.94 vs. 166.54 for foie gras weight (g), P < 0.05; 6.37 vs. 2.92% for the ratio of liver to body, P < 0.05; 60.01 vs. 46.64% for fat content, P < 0.05) and the less subcutaneous adipose tissue weight (1240.96 g vs. 1440.46 g, P < 0.05). After force-feeding, the digestion–absorption capacity of Tianfu Meat Goose was higher than that of Gang Goose (5.56 vs. 3.64 and 4.63 vs. 3.68 for the ratio of villus height to crypt depth in duodenum and ileum, respectively, P < 0.05; 1394.96 vs. 782.59 and 1314.76 vs. 766.17 for the invertase activity (U/mg-prot), in duodenum and ileum, respectively, P < 0.05; 6038.36 vs. 3088.29 and 4645.29 vs. 3927.61 for the activity of maltase (U/mg-prot), in duodenum and ileum, respectively, P < 0.05). Force-feeding decreased the gene expression of Escherichia coli in the ileum of Tianfu Meat Goose; force-feeding increased the number of gut microbiota Enterobacterial Repetitive Intergenic Consensus-Polymerase Chain Reaction band in Tianfu Meat Goose and decreased the number in Gang Goose. In conclusion, compared with Gang Goose, the lipid deposition in the liver and the intestine digestion–absorption capacity and stability were higher in Tianfu Meat Goose. Thereby, Tianfu Meat Goose is the better breed for foie gras production for prolonged force-feeding; Gang Goose possesses better fat storage capacity in subcutaneous adipose tissue. However, Gang Goose has lower gut stability responding to force-feeding, so Gang Goose is suited to force-feeding in a short time to gain the body weight and subcutaneous fat as an overfed duck for roast duck.

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Dietary infection of Enterobacter ludwigii causes fat accumulation and resulted in the diabetes-like condition in Drosophila melanogaster

Cited by 11 publications

References 79 publications

Effects of vancomycin‐induced gut microbiome alteration on the pharmacodynamics of metformin in healthy male subjects

Effects of vancomycin‐induced gut microbiome alteration on the pharmacodynamics of metformin in healthy male subjects

In Vivo Toxicological Analysis of MnFe₂O₄@poly(tBGE-alt-PA) Composite as a Hybrid Nanomaterial for Possible Biomedical Use

Comparison of overfeeding effects on gut physiology and microbiota in two goose breeds

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Dietary infection of Enterobacter ludwigii causes fat accumulation and resulted in the diabetes-like condition in Drosophila melanogaster

Cited by 11 publications

References 79 publications

Effects of vancomycin‐induced gut microbiome alteration on the pharmacodynamics of metformin in healthy male subjects

Effects of vancomycin‐induced gut microbiome alteration on the pharmacodynamics of metformin in healthy male subjects

In Vivo Toxicological Analysis of MnFe2O4@poly(tBGE-alt-PA) Composite as a Hybrid Nanomaterial for Possible Biomedical Use

Comparison of overfeeding effects on gut physiology and microbiota in two goose breeds

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Product

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In Vivo Toxicological Analysis of MnFe₂O₄@poly(tBGE-alt-PA) Composite as a Hybrid Nanomaterial for Possible Biomedical Use