Dietary n-3 polyunsaturated fatty acid supplementation alters the expression of genes involved in the control of fertility in the bovine uterine endometrium

Waters, Sinéad M.; Coyne, Gerard S.; Kenny, David A.; MacHugh, David E.; Morris, Deborah

doi:10.1152/physiolgenomics.00065.2011

Cited by 32 publications

(27 citation statements)

References 94 publications

(126 reference statements)

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“…tissues during the estrous cycle and peri-implantation periods in several species[68,[94][95][96][97][98].4.1. ConclusionsIn summary, LDs are differentially distributed in uterine compartments, and LDs expression in EG and LE are affected by nutritional plane, Arg or FSH in vivo treatments, and depends on stage of the estrous cycle in sheep.…”

mentioning

confidence: 99%

Lipid droplets in the ovine uterus during the estrous cycle: Effects of nutrition, arginine, and FSH

et al. 2017

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The aim of this study was to evaluate lipid droplet (LD) expression in uteri of FSH-treated or nontreated sheep administered with arginine (Arg) or vehicle (saline, Sal) and fed a control (C), excess (overfed, O) or restricted (underfed, U) diet. In experiment 1, ewes from each diet were randomly assigned to Arg or Sal treatments administered three times daily starting on Day 0 of the first estrous cycle until blood sample and uterine tissue collection at the early- or mid-luteal phase of the second estrous cycle or the late-luteal phase of the first estrous cycle. In experiment 2, ewes were injected twice daily with FSH on Days 13 to 15 of the first estrous cycle, and blood samples and uterine tissue were collected at the early- and mid-luteal phases of the second estrous cycle. Cryopreserved in optimum cutting temperature (OCT) compound, cross-sections of uterine horn were stained with boron-dipyrromethene (BODIPY; marker of LDs) followed by 4',6-diamidino-2-phenylindole (DAPI) staining and image analysis to determine the proportion (%) of area occupied by LD in luminal epithelium (LE) and endometrial glands (EGs). Control ewes maintained, O ewes gained, and U ewes lost body weight during the experiments. Serum progesterone concentration was not affected by nutritional plane or Arg treatment and was 5.5-fold greater in FSH- than Sal-treated ewes. LDs were detected in LE and superficial EG (close to LE) but not deep EG, or other uterine compartments, and area occupied by LD was greater in LE than in EG. In experiment 1, in LE and EG, area occupied by LDs was greater in C than in O or U; greater in Arg than in Sal; and greater at the late-, less at mid-, and least at early-luteal phases. In experiment 2, in LE and EG, area occupied by LDs was greater at mid- than in early-luteal phase. Comparison of data from FSH-treated and nontreated ewes (e.g., experiment 1 vs. experiment 2) demonstrated that FSH increased the area occupied by LD in LE and EG regardless of diet. Interactions between FSH treatment, stage of the estrous cycle, and plane of nutrition demonstrated that FSH increased the area occupied by LD in LE and EG at the mid-luteal phase in O and U. Thus, LDs are differentially distributed in uterine compartments, and area occupied by LD in endometrium is affected by nutritional plane, Arg or FSH, and stage of the estrous cycle. Such changes in dynamics of LD in the endometrium during the estrous cycle indicate their specific role in uterine functions.

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confidence: 99%

Lipid droplets in the ovine uterus during the estrous cycle: Effects of nutrition, arginine, and FSH

et al. 2017

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“…Body condition changes during transition have been associated with milk yield, post-partum health, and decreased fertility [42][43][44]. Few studies on RPF supplementation have included BCS as a response variable.…”

Section: Discussionmentioning

confidence: 99%

“…Negative energy balance in dairy cows is associated with reductions on luteinizing hormone pulse frequency, growth rate and diameter of dominant follicle, weight of the corpus luteum, estradiol, and progesterone [28,41]. Besides the increment of energy density, polyunsaturated FA influence fertility in farm animals by modulating the biosynthesis of prostaglandins, steroids, and the transcriptional regulation of genes involved in the control of fertility [42,43]. The FA content of the organic RPF used in this study was formulated to match the FA profile of the RPF available in the US market.…”

Section: Discussionmentioning

confidence: 99%

The effect of an organic rumen-protected fat supplement on performance, metabolic status, and health of dairy cows

Manríquez

Chen²,

Meléndez³

et al. 2019

Preprint

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Background Negative energy balance during the transition period is a concern for both conventional and certified organic dairy systems. During early lactation, supplemental strategies are used to cope with nutrient deficiencies that are associated with impaired health, production, and reproduction. As organic certified dairies in the United States face restricted access to dietary supplements, the evaluation of products especially formulated for organic production is needed. Our objective was to assess the effect of supplementing 0.45 kg/d of an organic rumen-protected fat (RPF) on health, metabolic status, and productive and reproductive performance of organic certified Holstein cows from 1 to 150 days in milk (DIM). Two-hundred and two cows were enrolled in a randomized blocked controlled trial conducted from January to July, 2017 in a certified organic dairy located in Northern Colorado (USA). Two groups were randomly assigned to be individually supplemented with organic RPF (ORG; n = 100) or control pellets (CON; n = 102) once per day, in addition to the total mixed ration (TMR). Outcomes of interest included disease occurrence, culling, mortality, serum concentration of glucose and non-esterified fatty acids (NEFA), milk yield (kg/d) and milk components, resumption of cyclicity, time-to-first artificial insemination (AI), conception at first AI, and conception within 150 DIM. Results A significant effect for the inclusion of RPF was found in daily milk yield; RPF supplemented cows had greater milk yield (1.6 kg/d) compared to CON cows up to 150 DIM (P = 0.03). During grazing, multiparous (MP) ORG cows had greater milk yield compared to MP CON cows, whereas no effect was found in primiparous (PP) cows. Health outcomes, serum metabolite concentrations, and reproductive performance were not affected by the inclusion of RPF. Body condition loss was smaller in the ORG group up to 80 DIM; however, there was no effect on body condition during the grazing season and in the overall study period. Conclusions These results indicate that supplementation of RPF increased daily milk yield and prevented body condition loss during transition. However, RPF supplementation did not affect health, serum metabolite concentration, milk components, and reproductive outcomes.

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“…Numerous different in vivo models exist that address endometrial function. These comprise the analysis of different time points during the cycle and early pregnancy, substitution of hormones in ovariectomised animals and application of substances orally, systemically, intravaginally or into the uterus (Kimmin and MacLaren 2001;Emond et al 2004;Forde et al 2009;Ulbrich et al 2010;Van Soom et al 2010;Dorniak et al 2011;Waters et al 2012). One particularly interesting model has been the use of identical twins generated by embryo splitting as recipient cows for embryo transfer to exclude a maternal genetic effect (Klein et al 2006).…”

Section: Models For Analysing Embryo]maternal Interactionsmentioning

confidence: 99%

Hosting the preimplantation embryo: potentials and limitations of different approaches for analysing embryo - endometrium interactions in cattle

Ulbrich

Wolf

Bauersachs

2013

Reprod. Fertil. Dev.

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Ongoing detailed investigations into embryo-maternal communication before implantation reveal that during early embryonic development a plethora of events are taking place. During the sexual cycle, remodelling and differentiation processes in the endometrium are controlled by ovarian hormones, mainly progesterone, to provide a suitable environment for establishment of pregnancy. In addition, embryonic signalling molecules initiate further sequences of events; of these molecules, prostaglandins are discussed herein as specifically important. Inadequate receptivity may impede preimplantation development and implantation, leading to embryonic losses. Because there are multiple factors affecting fertility, receptivity is difficult to comprehend. This review addresses different models and methods that are currently used and discusses their respective potentials and limitations in distinguishing key messages out of molecular twitter. Transcriptome, proteome and metabolome analyses generate comprehensive information and provide starting points for hypotheses, which need to be substantiated using further confirmatory methods. Appropriate in vivo and in vitro models are needed to disentangle the effects of participating factors in the embryo-maternal dialogue and to help distinguish associations from causalities. One interesting model is the study of somatic cell nuclear transfer embryos in normal recipient heifers. A multidisciplinary approach is needed to properly assess the importance of the uterine milieu for embryonic development and to use the large number of new findings to solve long-standing issues regarding fertility.

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Dietary n-3 polyunsaturated fatty acid supplementation alters the expression of genes involved in the control of fertility in the bovine uterine endometrium

Abstract: Waters SM, Coyne GS, Kenny DA, MacHugh DE, Morris DG. Dietary n-3 polyunsaturated fatty acid supplementation alters the expression of genes involved in the control of fertility in the bovine uterine endometrium.

Cited by 32 publications

References 94 publications

Lipid droplets in the ovine uterus during the estrous cycle: Effects of nutrition, arginine, and FSH

Lipid droplets in the ovine uterus during the estrous cycle: Effects of nutrition, arginine, and FSH

The effect of an organic rumen-protected fat supplement on performance, metabolic status, and health of dairy cows

Hosting the preimplantation embryo: potentials and limitations of different approaches for analysing embryo - endometrium interactions in cattle

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