Dietary non-phytate phosphorus requirement of broilers fed a conventional corn-soybean meal diet from 1 to 21 d of age

Liu, S. B.; Liao, Xiudong; Lü, Lin; Li, S. F.; Wang, L.; Zhang, L. Y.; Jiang, Yong; Luo, Xugang

doi:10.3382/ps/pew212

Cited by 58 publications

(49 citation statements)

References 52 publications

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“…The sample size calculation was performed using software PASS 13 (NCSS, LLC). In our previous study (17) , the means of duodenal NaPi-IIb mRNA expression levels for moderately restricted and normal NPP level treatments were 2•32 and 1•03 with a pooled SD of 0•63. On the basis of the same effect size, five replicates per treatment are needed to achieve 80 % power using a two-sided two-sample t test at a significance level of 0•05.…”

Section: Statistical Analysesmentioning

confidence: 81%

Phosphorus absorption and gene expression levels of related transporters in the small intestine of broilers

Liao

Wen

et al. 2018

Br J Nutr

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To investigate the P absorption and gene expression levels of related co-transporters, type IIb sodium-dependent phosphate co-transporter (NaPi-IIb), inorganic phosphate transporter 1 (PiT-1) and inorganic phosphate transporter 2 (PiT-2) in the small intestine of broilers, 450 1-d-old Arbor Acres male broilers were randomly allocated to one of three treatments with ten replicate cages of fifteen birds per cage for each treatment in a completely randomised design. Chickens were fed a diet with no added inorganic P (containing 0·06 % non-phytate P (NPP)) or with either 0·21 or 0·44 % NPP for 21 d. Plasma P concentration in the hepatic portal vein, mRNA and protein expression levels of NaPi-IIb, PiT-1 and PiT-2 were determined at 7, 14 and 21 d of age. The results showed that the concentration of P in plasma in the hepatic portal vein increased as dietary NPP increased (P<0·0001). At 14 and 21 d of age, the increase in dietary NPP inhibited (P<0·003) NaPi-IIb mRNA expression level in the duodenum, as well as PiT-1 mRNA and protein expression levels in the ileum, but promoted NaPi-IIb protein expression level (P<0·002) and PiT-2 mRNA and protein expression levels (P<0·04) in the duodenum. These results suggest that NaPi-IIb, PiT-1 and PiT-2 might be important P transporters in the small intestine of broilers. Higher intestinal P absorption may be achieved by up-regulating the protein expression levels of NaPi-IIb and PiT-2 and down-regulating the protein expression of PiT-1.

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Section: Statistical Analysesmentioning

confidence: 81%

Phosphorus absorption and gene expression levels of related transporters in the small intestine of broilers

Liao

Wen

et al. 2018

Br J Nutr

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“…Many studies demonstrated that the bone development parameters, such as tibia BMD, BBS, and ash content of broilers, were susceptible to dietary Ca or P level ( Williams et al., 2000a , Rama Rao et al., 2003 , Liu et al., 2017 ). Results from the present study showed that the Ca- or P-deficient diets negatively affected the tibia BMD, BBS, and ash content of broilers.…”

Section: Discussionmentioning

confidence: 99%

“…The frozen tibiae were thawed at room temperature for 2 h and then stripped of all soft tissues. The tibia BMD ( Liu et al., 2017 , Jing et al., 2018 ) was determined by dual-energy X-ray absorptiometry. The tibia BBS was determined by a 3-point bending test (HDP/3PB Texture Analyzers, West Sussex, UK).…”

Section: Methodsmentioning

confidence: 99%

“…The breaking force was determined by the shear test at a speed of 5 mm/min with a 50 kg loading cell until fracture occurred ( Crenshaw et al., 1981 , Shim et al., 2012 , Sadeq et al., 2018 ). The ultimate tibia breaking force was directly obtained from the loaded-deformation curve recorded by a computerized monitor ( Jiang et al., 2016 , Liu et al., 2017 ). The tibia bone was dried using an oven at 105°C for 24 h and then defatted with fresh diethyl ether for 48 h. The fat-free, dried bone was finally ashed using a muffle furnace at 550°C for 16 h. The tibia ash content was expressed on a dried and defatted weight basis of tibia.…”

Section: Methodsmentioning

confidence: 99%

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Dietary calcium or phosphorus deficiency impairs the bone development by regulating related calcium or phosphorus metabolic utilization parameters of broilers

Xing

Shao

et al. 2020

Poultry Science

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An experiment was conducted to investigate the effect of dietary calcium ( Ca ) or phosphorus ( P ) deficiency on bone development and related Ca or P metabolic utilization parameters of broilers. A total of 504 one-day-old Arbor Acres male broilers were randomly assigned to 1 of 4 treatments with 7 replicates of 18 birds per replicate in a completely randomized design. A 2 (Ca levels: 1.00 and 0.35%) × 2 (nonphytate P [ NPP ] levels: 0.45 and 0.23%) factorial arrangement of treatments was adopted in the 21-day trial. The 4 treatments were the Ca- and P-adequate diet (1.00% Ca + 0.45% NPP), the Ca-deficient diet (0.35% Ca + 0.45% NPP), the P-deficient diet (1.00% Ca + 0.23% NPP), and the Ca- and P-deficient diet (0.35% Ca + 0.23% NPP). The greatest impact on tibia bone mineral density, bone breaking strength, and ash content was in the P-deficient diets, especially in broilers fed with the Ca-adequate diet, whereas adequate P and reduced Ca reduced ( P < 0.05) these parameters compared with adequate Ca and P, but not to the same level as P deficiency. Furthermore, dietary Ca or P deficiency, especially adequate Ca and P deficiency decreased ( P < 0.05) serum P, 25-hydroxyvitamin D 3 ( 25-OHD 3 ) contents, and tibia ash Ca and P contents but increased ( P < 0.05) the serum Ca content and tibia alkaline phosphatase ( ALP ) activity compared with adequate Ca and P. The results from this study indicated that the bone development and Ca or P metabolic utilization parameters of broilers were the most sensitive ones to dietary P deficiency, followed by dietary Ca deficiency or Ca and P deficiencies. Dietary P deficiency impaired the bone development by increasing serum Ca content and tibia ALP activity but decreasing serum P, 25-OHD 3 contents, and tibia ash Ca and P contents of broilers. Dietary Ca deficiency impaired bone development by increasing serum Ca content, tibia ALP activity, and tibia ash P content but decreasing serum P, 25-OHD 3 contents, and tibia ash Ca content of broilers.

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“…The right tibiae was cleaned and dried for determination of tibia weight and tibia breaking strength [22]. The bones were then ashed in a mu e furnace at 550 °C for 16 h [19]. After that, the tibia P content was measured using the vanadate-molybdate method.…”

Section: Experimental Designmentioning

confidence: 99%

Enterococcus Faecium Modulates the Gut Microflora of Broilers and Enhances Phosphorus Absorption and Utilization

wang

Cai

Zhang

et al. 2020

Preprint

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Background: The modern broiler or meat chicken has ongoing bone health problems. Phosphorus (P) plays an important role in bone development and increased understanding of P metabolism should improve the skeletal health of broilers. Enterococcus faecium has been widely used as a probiotic in broiler production and shown to improve skeletal health of rats; but its effect on the bones of broilers remains unclear. This study investigated the effect of E. faecium on P absorption and utilization in broilers and associated changes in the gut microflora using 16S rDNA sequencing.Results: Dietary supplementation with E. faecium improved P absorption through up-regulation of the expression of intestinal NaP-IIb mRNA, and increased the concentration of serum alkaline phosphatase. These actions increased P retention and bone mineralization in E. faecium treated broilers. The positive effects of E. faecium on P metabolism were associated with changes in the structure of the intestinal microbiota. There was increased relative abundance of the following genera, Alistipes, Eubacterium, Rikenella and Ruminococcaceae, and a decrease in the relative abundance of Faecalibacterium and Escherichia-Shigella. Conclusions: Dietary supplementation with E. faecium changed gut microbiota structure of broilers, increased the relative abundance of SCFA (short chain fatty acid) producing bacteria, improved intestinal P absorption, bone forming metabolic activities, and decreased P excretion. E. faecium facilitates increased utilisation of P in broilers.

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Dietary non-phytate phosphorus requirement of broilers fed a conventional corn-soybean meal diet from 1 to 21 d of age

Cited by 58 publications

References 52 publications

Phosphorus absorption and gene expression levels of related transporters in the small intestine of broilers

Phosphorus absorption and gene expression levels of related transporters in the small intestine of broilers

Dietary calcium or phosphorus deficiency impairs the bone development by regulating related calcium or phosphorus metabolic utilization parameters of broilers

Enterococcus Faecium Modulates the Gut Microflora of Broilers and Enhances Phosphorus Absorption and Utilization

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