Dietary polyunsaturated fatty acids significantly affect laying hen lymphocyte proliferation and immunoglobulin G concentration in serum and egg yolk

Abstract

. 2000. Dietary polyunsaturated fatty acids significantly affect laying hen lymphocyte proliferation and immunoglobulin G concentration in serum and egg yolk. Can. J. Anim. Sci. 80: 597-604. Forty eight (48) Single Comb White Leghorn laying hens 24 wk of age were housed in cages and were fed wheat-soybean meal based diets with added oils [sunflower oil (SO), animal oil (AO), linseed oil (LO), or fish oil (FO)] at 5%. After 5 wk on experimental diets, spleen lymphocytes and peripheral blood lymphocytes were obtained from six birds and assayed for polyclonal mitogen Con A-induced proliferative response and the proportions of lymphocyte subsets. The IgG concentration in serum and egg yolk was also measured. Feeding LO and FO resulted in an increase in longer-chain n-3 polyunsaturated fatty acids (PUFA) (C20:5n-3, C22:5n-3, and C22:6n-3) with a concurrent decrease of C20: 4n-6 in spleen lymphocytes (P < 0.05). The highest enrichments of the longer-chain n-3 PUFA were achieved by feeding FO. The content of C20: 4n-6 was higher (P < 0.05) in the lymphocytes of hens fed AO and SO. Subsequently, Con A-stimulated proliferation of spleen and peripheral blood lymphocytes were significantly suppressed (P < 0.05) in the chicks fed high n-3 PUFA diets (LO and FO). The LO diet increased (P < 0.05) the IgG concentration in laying hen serum. The SO diet reduced (P < 0.05) IgY content in egg yolk. Dietary fatty acids did not affect (P > 0.05) the proportions of lymphocyte subsets in spleen and blood lymphocytes. It is indicated that the ratio of n-6 to n-3 PUFA plays a major role in modulating cell-mediated and humoral immune responses of laying hens, and various n-3 fatty acids possess different potencies of immunomodulation. Quarante-huit (48) poules de ponte White Leghorn à crête simple, âgées de 24 semaines étaient logées en cage, et exposées à des régimes alimentaires base de blé-tourteau de soja, additionnés, à raison de 5%, de diverses huiles ou graisses: tournesol (HT), lin (HL), suif (S) ou poisson (HP). Au bout de 5 semaines en régime expérimental, nous avons obtenu à partir de 6 poules des lymphocytes de la rate et du sang périphérique, dont nous avons analysé la réponse proliférative induite par le mitogène polyclonal Con-A, ainsi que les proportions des sous-types lymphocytaires. On mesurait également la concentration en IgG du sérum et du jaune d'oeuf. L'apport de HL et de HP déclenchait l'augmentation des AGPI à chaîne longue : C20 :5n-3, C22 :5n-3 et C22 :6n-3 et la diminution concomitante de C20 :4n-6 dans les lymphocytes de la rate (P < 0,05). C'est l'huile de poisson qui produisait les accroissements les plus remarquables des AGPI à chaîne longue. La teneur en C20 : 4n-6 était plus élevée (P < 0,05) dans les lymphocytes des poules recevant le suif ou HT. Par la suite, la prolifération activée par CON-a des lymphocytes de la rate et du sang périphérique manifestait une inhibition significative (P < 0,05) chez les poulettes recevant les aliments riches en AGPI n-3, c.-à-d. HL et HP. Le régime à HL donnait lieu à ...

Dietary n‐3 fatty acids reduce the delayed hypersensitivity reaction and antibody production more than n‐6 fatty acids in broiler birds

“…The present study showed a modulation of immune tissue fatty acid composition and indices of immune responses by dietary cis and trans PUFA. The results reported on n-3 and n-6 fatty acid composition of splenocytes are consistent with previously reported work with poultry [9]. To our knowledge, the effect of CLA on broiler splenocytes has not been reported.…”

“…Fritsche et al [14] reported an increased antibody titer against BSA in laying birds fed fish oil but not flaxseed oil. Wang et al [9] reported an increase in total antibody production of layer birds fed flaxseed oil, but not fish oil. Enhanced serum antibody production was also observed by Friedman and Sklan [4] in broiler chicks fed a high n-3 PUFA diet and challenged with BSA.…”

“…Cells at the interface of density separation medium and the CCM were collected, washed twice with CCM and resuspended in 5 mL of 5% fetal bovine serum-CCM (FBS-CCM). Resuspended lymphocytes were counted using a haemocytometer and the cell suspension diluted to a final concentration of 1 × 10 7 cells in 5% FBS-CCM [9].…”

Effect of dietary manipulation and vaccination of turkey breeder hens on immunoglobulin levels of yolk, yolk sac and neonate poults

“…Jennifer, Gregory, and Ellen () compared a low protein diet and a control diet and concluded that dietary quality did not affect the immunoglobulin concentration of eggs. Contrary to this, Wang, Cherian, Sunwoo, and Sim () reported that sunflower oil reduced IgY content in egg yolks and they suggested that the ratio of n‐6 to n‐3 polyunsaturated fatty acids influenced chick humoral immune response. In our study, the high immune diet comprised of amino acids 115% of NRC, 2% higher fatty acid than the basal diet (1% each omega‐6 and omega‐3 fatty acids), vitamin A‐15,000 IU, vitamin E‐200 IU, vitamin C—150 mg, selenium—0.5 mg and zinc—120 mg per kg diet for 8‐week duration.…”

EFECTO DEL ACEITE DE SACHA INCHI (Plukenetia volubilis) EN LA DIETA DE REPRODUCTORAS DE POLLOS DE ENGORDE SOBRE EL DESEMPEÑO PRODUCTIVO DE SU PROGENIE

“…También tiene un efecto positivo sobre los niveles de anticuerpos maternales, lo que podría aumentar la productividad (Ajuyah et al, 2003; Wang et al, 2004;Cherian, 2007). Se ha demostrado que el ALA estimula la unión de la IgY con su receptor en la membrana del saco vitelino, incrementando la transferencia maternal embrionaria de anticuerpos maternales (Wang et al, 2000;Wang, 2001). …”