Dietary Supplementation of Apple Phlorizin Attenuates the Redox State Related to Gut Microbiota Homeostasis in C57BL/6J Mice Fed with a High-Fat Diet

Liu, Dong; Ji, Yanglin; Guo, Yatu; Wang, Hui; Wu, Zijian; Li, Heyu; Wang, Hao

doi:10.1021/acs.jafc.0c06426

Cited by 29 publications

(31 citation statements)

References 52 publications

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“…Information on the vitamin mix, mineral mix, casein, lard, corn starch, sucrose, gelatin, formic acid, acetonitrile, and other reagents was mentioned in our previous study (Liu, Ji, Guo, et al., 2021).…”

Section: Methodsmentioning

confidence: 99%

“…Finally, the concentrated and lyophilized eluent was purified with ethyl acetate, and the yield of PSPAE was approximately 0.23 g from 100 g raw purple sweet potato (approximately 0.71 g per 100 g dry weight powder). Information on the vitamin mix, mineral mix, casein, lard, corn starch, sucrose, gelatin, formic acid, acetonitrile, and other reagents was mentioned in our previous study (Liu, Ji, Guo, et al, 2021).…”

Section: Materials and Reagentsmentioning

confidence: 99%

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Purple sweet potato anthocyanin extract regulates redox state related to gut microbiota homeostasis in obese mice

Liu

Wang

et al. 2022

Journal of Food Science

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This study explored the advantageous effects of purple sweet potato anthocyanin extract (PSPAE) on redox state in obese mice. The normal chow diet (NCD) group, high‐fat/cholesterol diet (HCD) group, and three groups based on HCD and added with low, middle, and high dose of PSPAE (PAL, PAM, and PAH) were raised for 12 weeks. High dose of PSPAE treatment decreased the elevations of the body weight by 24.7%, serum total cholesterol by 48.3%, serum triglyceride by 42.4%, and elevated serum activities of glutathione peroxidase by 53.3%, superoxide dismutase by 57.8%, catalase by 75.4%, decreased serum contents of malondialdehyde by 27.1% and lipopolysaccharides by 40.5%, as well as increased caecal total short‐chain fatty acid by 2.05‐fold. Additionally, PSPAE depressed toll‐like receptor 4 (TLR‐4), nuclear factor kappa‐B (NF‐κB), interleukin 6, tumor necrosis factor α, and preserved nuclear factor erythroid‐2‐related factor 2 (Nrf2) gene expression. Similarly, the protein expression of Nrf2 was enhanced, while TLR‐4 and p‐NF‐κB/NF‐κB were depressed by PSPAE treatment. Moreover, PSPAE administration promoted the protection of intestinal barrier function and rebuilt gut microbiota homeostasis by blooming g_Akkermansia, g_Bifidobacterium, and g_Lactobacillus. Furthermore, antibiotic interference experiments showed that the gut microbiota was indispensable for preserving the redox state of PSPAE. These results suggested that PSPAE administration could be an opportunity for improving HCD‐induced obesity and the redox state related to gut dysbiosis. Practical Application Purple sweet potato anthocyanin has diverse pharmacological properties. It is applicable for individuals to consume extracts (as pills or other forms) from raw purple sweet potato if they want to improve obesity or redox state.

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Section: Methodsmentioning

confidence: 99%

Section: Materials and Reagentsmentioning

confidence: 99%

Purple sweet potato anthocyanin extract regulates redox state related to gut microbiota homeostasis in obese mice

Liu

Wang

et al. 2022

Journal of Food Science

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“…These results suggested that the administration of FLRB could increase the content of SCFAs in diabetic mice and might account for the change in gut microbiota in this study. SCFAs were proven to play a crucial role in gut barrier function by improving epithelial growth and can impede appetite by upregulating several proteins related to satiety, and they were closely correlated with the structure of intestinal microbes ( Kim et al, 2018 ; Liu et al, 2021 ). In our study, we showed that the treatment of defatted rice bran fermentation extracts not only enriched SCFA-producing bacteria but also increased the content of SCFAs in diabetic mice, suggesting that the major modulation of intestinal microbes may occur by changing the production of SCFAs.…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, these results suggested that the gut microbiota played key roles in the development of T2DM. Short-chain fatty acid (SCFA), generated from undigested carbohydrates by gut microbiota, strengthen gut barrier function by supporting energy for epithelial growth and resisting invading microbes ( Wang Y. X. et al, 2020 ; Liu et al, 2021 ). Moreover, SCFAs can regulate the relative expression of proteins controlling satiety and then reduce appetite to improve T2DM ( Kim et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Antidiabetic Function of Lactobacillus fermentum MF423-Fermented Rice Bran and Its Effect on Gut Microbiota Structure in Type 2 Diabetic Mice

Yin

et al. 2021

Front. Microbiol.

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Rice bran is an industrial byproduct that exerts several bioactivities despite its limited bioavailability. In this study, rice bran fermented with Lactobacillus fermentum MF423 (FLRB) had enhanced antidiabetic effects both in vitro and in vivo. FLRB could increase glucose consumption and decrease lipid accumulation in insulin resistant HepG2 cells. Eight weeks of FLRB treatment significantly reduced the levels of blood glucose and lipids and elevated antioxidant activity in type 2 diabetic mellitus (T2DM) mice. H&E staining revealed alleviation of overt lesions in the livers of FLRB-treated mice. Moreover, high-throughput sequencing showed notable variation in the composition of gut microbiota in FLRB-treated mice, especially for short-chain fatty acids (SCFAs)-producing bacteria such as Dubosiella and Lactobacillus. In conclusion, our results suggested that rice bran fermentation products can modulate the intestinal microbiota and improve T2DM-related biochemical abnormalities, so they can be applied as potential probiotics or dietary supplements.

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“…The byproducts of lipid metabolism in a HFD would injure the mucosa of the large intestine and decrease the mucosal thickness of the cecum ( Yang et al, 2021 ). A recent report has shown that infiltration of inflammatory cells, ulceration, and epithelial damage were also detected in the cecum and colon of mice fed with HFD ( Liu et al, 2021 ). In addition, gut microbiota mainly in cecum and colon could utilize dietary fiber or other macromolecule, which is not digested by host enzyme, to produce some effective metabolites ( Koh et al, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Ginsenoside Rb1 ameliorates Glycemic Disorder in Mice With High Fat Diet-Induced Obesity via Regulating Gut Microbiota and Amino Acid Metabolism

et al. 2021

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Accumulating evidences suggested an association between gut microbiome dysbiosis and impaired glycemic control. Ginsenoside Rb1 (Rb1) is a biologically active substance of ginseng, which serves anti-diabetic effects. However, its working mechanism especially interaction with gut microbes remains elusive in detail. In this study, we investigated the impact of Rb1 oral supplementation on high fat diet (HFD) induced obesity mice, and explored its mechanism in regulating blood glucose. The results showed that higher liver weight and lower cecum weight were observed in HFD fed mice, which was maintained by Rb1 administration. In addition, Rb1 ameliorated HFD induced blood lipid abnormality and improved insulin sensitivity. Several mRNA expressions in the liver were measured by quantitative real-time PCR, of which UCP2, Nr1H4, and Fiaf were reversed by Rb1 treatment. 16S rRNA sequencing analysis indicated that Rb1 significantly altered gut microbiota composition and increased the abundance of mucin-degrading bacterium Akkermansia spp. compared to HFD mice. As suggested via functional prediction, amino acid metabolism was modulated by Rb1 supplementation. Subsequent serum amino acids investigation indicated that several diabetes associated amino acids, like branched-chain amino acids, tryptophan and alanine, were altered in company with Rb1 supplementation. Moreover, correlation analysis firstly implied that the circulation level of alanine was related to Akkermansia spp.. In summary, Rb1 supplementation improved HFD induced insulin resistance in mice, and was associated with profound changes in microbial composition and amino acid metabolism.

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Dietary Supplementation of Apple Phlorizin Attenuates the Redox State Related to Gut Microbiota Homeostasis in C57BL/6J Mice Fed with a High-Fat Diet

Cited by 29 publications

References 52 publications

Purple sweet potato anthocyanin extract regulates redox state related to gut microbiota homeostasis in obese mice

Purple sweet potato anthocyanin extract regulates redox state related to gut microbiota homeostasis in obese mice

Antidiabetic Function of Lactobacillus fermentum MF423-Fermented Rice Bran and Its Effect on Gut Microbiota Structure in Type 2 Diabetic Mice

Ginsenoside Rb1 ameliorates Glycemic Disorder in Mice With High Fat Diet-Induced Obesity via Regulating Gut Microbiota and Amino Acid Metabolism

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