Diferentes Concentrações De Gema De Ovo Em Pó Adicionada Ao Diluente Acp-103® Na Conservação Do Sêmen Suíno

Toniolli, Ricardo; Barros, Tatyane Bandeira; Toniolli, Luciana de Souza; Guimarães, Daianny Barboza; Freitas, Eduardo Nunes de; Nunes, Thalles Gothardo Pereira

doi:10.1590/1089-6891v17i217643

Cited by 2 publications

(2 citation statements)

References 20 publications

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“…Em adição, tratamentos em que o diluente é o Tris Gema há uma maior taxa de preservação da membrana da célula espermática a qual permanece com sua membrana normal do ponto de vista funcional, mesmo na presença de DMSO no diluente. Situação semelhante foi também relatada por outros autores [13,26] os quais mencionam que diluidores a base de gema de ovo determinam uma maior preservação da membrana plasmática, pois tem em sua composição frações lipoproteicas de baixa densidade que ajudam a preservar a Tabela 1. Características espermáticas do pool de sêmen de cinco reprodutores caprinos, refrigerado e diluído em meio essencial mínimo (MEM) ou Tris Gema a 2,5%, acrescidos de concentrações crescentes de dimetilsufólxido (DMSO).…”

Section: Discussionunclassified

Evaluation of Solvent Toxicity of Plant Extract with Antiviral Action in Refrigerated Goat Semen

Peixoto¹,

Andrioli

Santos

et al. 2017

Acta Scientiae. Vet.

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Background: Caprine Arthritis Encephalitis Virus have been detected in sperm of breeding goats causing economic losses. In order to control the virus, researches aiming to identify natural extracts with potential antiviral effects are performed. However, aqueous or ethanolic extracts must be diluted in dimethyl sulfoxide (DMSO), which is a substance with unknown effects in sperm quality when present in diluting media. Therefore, this study aimed to evaluate sperm viability of refrigerated caprine semen diluted in media containing DMSO. This was performed to provide data that aid in researches involving the use of this component with natural extracts that may inactivate the caprine lentivirus in sperm.Materials, Methods & Results: The experiment was performed at the Laboratory of Seminal Technology in Embrapa Goats and Sheep in the city of Sobral, Brazil. Sperm viability was assessed in caprine semen refrigerated in two dilution media with crescent concentrations of DMSO. Sperm samples of five goats seronegative for the caprine lentivirus were pooled and diluted in minimal essential medium (MEM) enriched with glucose at 0.01 M added of crescent concentrations of DMSO (0%, 1.5%, 1.75%, 2.0%, 2.25% and 2.5%). The same breeders provided the pool of sperm to test Tris added 2.5% of egg yolk and the same concentrations of DMSO previously mentioned. Treatments were refrigerated at 7°C and evaluated up until four h after DMSO addition. Individual progressive motility (MIP), sperm vigor (V), percentage of spermatozoa reactive to hypoosmotic test (HO) and morphologically normal (NOR) were evaluated. IPM, vigor and NOR remained within normal standards for the caprine species in all treatments test. Percentage results of spermatozoa reactive to hypoosmotic was higher in Tris yolk with values ranging between 34.66% to 46.33%. Sperm vigor was positively correlated (r = 0.85) with IPM in the MEM diluted pool of sperm. In Tris yolk, vigor and hypoosmotic test correlated moderately (r = 0.63, r = 0.54, respectively) with IPM. Tris yolk medium added DMSO presented the highest percentage of reactivity to hypoosmotic test in all treatments when compared to MEM added DMSO.Discussion: The fact that DMSO is easily homogenized in water, ethylic alcohol and most organic solvents favors its use in diluting natural extracts. These components are a possible source of products that inactivate caprine arthritis encephalitis virus in sperm, which is the key to promoting the safe use of genetic material of infected breeders, in addition to commercial use of germplasm. In this study, there was no interference of DMSO in the analyzed parameters when added in a maximum concentration of 2.5% to MEM and Tris yolk, which is in accordance with standard values for goats. In addition, Tris yolk may promote greater protection to the membrane of sperm cells, which was demonstrated by hypoosmotic test. This medium could be ideal to be used in new methodologies that incorporate DMSO. In conclusion, DMSO added to dilution media Tris yolk and MEM did not interfere with the quality of refrigerated caprine sperm, which maintained viability. These results indicate that this substance did not present harmful effects to the genetic material, promoting the use as solvent of extracts from plant compounds with potential anti-viral effect. The information in this study may aid new research performed in this area.

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Section: Discussionunclassified

Evaluation of Solvent Toxicity of Plant Extract with Antiviral Action in Refrigerated Goat Semen

Peixoto¹,

Andrioli

Santos

et al. 2017

Acta Scientiae. Vet.

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“…Estudos com adição de gema de ovo ao diluente apresentam resultados divergentes quando a qualidade espermática durante a conservação e criopreservação em diferentes espécies de mamíferos, incluindo suínos (Bathgate et al, 2006;Toniolli et al, 2016); bovinos (Wall e Foot, 1999); caprinos (Viana et al, 2006;Santiago-Moreno et al, 2008); búfalos (Andrabi et al, 2008), equinos (Bruemmert et al, 2002;Melo e Henry, 2005) e caninos (Silva et al, 2000;Iguer-Ouada e Verstegen, 2001;Moura et al, 2002;Cardoso et al, 2010). Além disso, a maioria dos trabalhos mencionou o uso de 20% de gema (Silva, 2000;Iguer-Ouada e Verstegen, 2001;Moura et al, 2002;Cardoso et al, 2010;Bispo et al, 2011), independente da composição do diluente base.…”

Section: Introductionunclassified

Refrigeração de sêmen canino com diluente a base de leite e gema de ovo 2%

et al. 2021

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O processo de conservação de sêmen sob resfriamento necessita de adição de diluentes, contendo diferentes formulações. Estudos com adição de gema de ovo ao diluente apresentam resultados divergentes quanto a qualidade espermática, durante a conservação e criopreservação em diferentes espécies de mamíferos. Diante disso, o objetivo deste experimento foi avaliar a qualidade espermática de sêmen canino adicionado de diluente a base de leite e gema de ovo de galinha a 2%, mantido sob resfriamento a 5ºC por 72h. Para isso, 20 ejaculados foram coletados de caninos e submetidos a dois tratamentos: 1) Diluente Sem Gema e 2) Diluente Com Gema de ovo de galinha a 2%. As amostras foram mantidas em geladeira a 5ºC e avaliadas as 24, 48 e 72h de resfriamento quanto a motilidade, vigor, integridade de membrana plasmática e morfologia. A análise estatística incluiu estatística descritiva, Shapiro–Wilk, Wilcoxon, análise de variância (ANOVA), comparação de médias pelo teste de Tukey. Os valores foram expressos em a média ± erro padrão da média. Foi verificada diferença significativa entre os tratamentos quanto à motilidade às 48h (Sem Gema 38,3±4,2% e Com Gema 51,5±4,7%) e 72h (Sem Gema 30±4,8% e Com Gema 38,4±5,1%) (p<0,05). O vigor, a integridade funcional da membrana plasmática e a morfologia espermática não foram significativamente afetados pelo tratamento. Em conclusão, os ejaculados de caninos adicionados de diluente acrescido de gema de ovo de galinha a 2% apresentaram maior percentual de motilidade durante resfriamento a 5ºC por 72h.

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Diferentes Concentrações De Gema De Ovo Em Pó Adicionada Ao Diluente Acp-103® Na Conservação Do Sêmen Suíno

Cited by 2 publications

References 20 publications

Evaluation of Solvent Toxicity of Plant Extract with Antiviral Action in Refrigerated Goat Semen

Evaluation of Solvent Toxicity of Plant Extract with Antiviral Action in Refrigerated Goat Semen

Refrigeração de sêmen canino com diluente a base de leite e gema de ovo 2%

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